Volume 9 (1993) Issue 1 - Issue 6
- D Thompson, P H Bartels, H G Bartels, P W Hamilton, J M Sloan
Knowledge-guided segmentation of colorectal histopathologic imagery
1993 Aug;15(4):236-46
Abstract: Scene segmentation in images of sections of colorectal glands is guided by a knowledge-based system. The construction of the knowledge file, processing sequences and computation of a crowding index for adenomatous glands are described. - A Moragas, C Castells, M Sans
Mathematical morphologic analysis of aging-related epidermal changes
1993 Apr;15(2):75-82
Abstract: Fractographic techniques based on mathematical morphology were used to study aging-related epidermal changes in abdominal skin samples obtained from 96 autopsy cases. Three linear roughness indices were evaluated for the rete peg profile and the shrinkage effect on the basal layer and interface between the granular and horny layers. - A Savarese, M Nuti, C Botti, M Cianfriglia, A Vecchione, G Zupi
Sample preparation for bivariate flow cytometric analysis of breast tumor specimens
1993 Feb;15(1):39-49
Abstract: The availability of monoclonal antibodies (MAbs) raised against functional or phenotypic cell markers can improve the suitability of flow cytometry (FCM) in assessing and validating prognostic parameters of human neoplasia. - B L Kuyatt, C A Reidy, K Y Hui, W H Jordan
Quantitation of smooth muscle proliferation in cultured aorta. A color image analysis method for the Macintosh
1993 Apr;15(2):83-7
Abstract: Color image analysis was used to assess proliferative changes in smooth muscle cells from cultured segments of rabbit aortas. Proliferating cells were labeled with bromodeoxyuridine (BrdU) and visualized by immunohistochemical staining of histologic sections. - G Landini, J W Rippin
Fractal dimensions of the epithelial-connective tissue interfaces in premalignant and malignant epithelial lesions of the floor of the mouth
1993 Apr;15(2):144-9
Abstract: Irregularity of the shape of epithelial-connective tissue interfaces is a well-recognized feature of malignant and premalignant epithelial lesions, yet few attempts have been made to assess it objectively. - T Masuda, T Kasai, R Satodate
Quantitative measurement of hemosiderin deposition in tissue sections of the liver by image analysis
1993 Dec;15(6):379-82
Abstract: Hemosiderin has been quantitated in tissue sections by image analysis employing Perls' Berlin blue reaction. A linear correlation was observed between the mean optical density of the tissue section measured with a commercial image analyzer and the iron concentration measured with an atomic absorption spectrophotometer. - H Harms, U Gunzer, I Baumann, S Serbouti
Malignancy-associated changes in monocytes and lymphocytes in acute leukemias measured by high-resolution image processing
Comparative Study: 1993 Dec;15(6):371-8
Abstract: A number of methods are available for classifying lymphoid and myeloid leukemias in peripheral blood and bone marrow. However, in clinical diagnosis an initial and particularly important step is morphologic analysis. - J Babiarz, J M Peters, B Miles, J D Crissman
Comparison of DNA content in primary and lymph node metastases in prostate adenocarcinoma
Comparative Study: 1993 Jun;15(3):158-64
Abstract: There is increasing evidence that nuclear DNA content has significant prognostic value for adenocarcinoma of the prostate. There also appear to be considerable differences in cellular DNA content between patient cohorts when primary tumor or pelvic lymph node metastases are measured. - R G Steinbeck, K M Heselmeyer, W F Neugebauer, U G Falkmer, G U Auer
DNA ploidy in human colorectal adenocarcinomas
Comparative Study: 1993 Jun;15(3):187-94
Abstract: Image cytometric determination of DNA content was performed on 207 colorectal adenocarcinomas surgically resected from 205 patients. Of these 207 tumors, 193 (93.2%) showed an aneuploid DNA distribution pattern, whereas the remaining 14 (6.8%) exhibited a DNA pattern corresponding to that found in proliferating diploid populations. - M Bibbo, P H Bartels, T Pfeifer, D Thompson, C Minimo, H G Davidson
Belief network for grading prostate lesions
1993 Apr;15(2):124-35
Abstract: A Bayesian belief network for grading prostatic lesions into eight primary Gleason grades was developed and tested. The network employs 13 diagnostic clues, 8 based on tissue architectural features and 5 based on nuclear features. - T L Gramlich, C Fritsch, S Shear, D Sgoutas, T Tuten, T Gansler
Analysis of epidermal growth factor receptor gene expression in stained smears and formalin-fixed, paraffin-embedded cell pellets by reverse transcription intron differential polymerase chain reaction
1993 Oct;15(5):317-22
Abstract: Previous studies have demonstrated quantitation of epidermal growth factor receptors (EGFR) to be of prognostic significance in breast, bladder, esophageal and other neoplasms. - J C Fleege, P J van Diest, J P Baak
Systematic random sampling for selective interactive nuclear morphometry in breast cancer sections. Refinement and multiobserver evaluation
Multicenter Study: 1993 Aug;15(4):281-9
Abstract: Interactive nuclear morphometry in histologic tumor sections is widely employed in quantitative pathology because it can add to objectivity with regard to the diagnosis and prognosis of cancer patients. - A E Dawson, E S Cibas, J W Bacus, D S Weinberg
Chromatin texture measurement by Markovian analysis. Use of nuclear models to define and select texture features
Comparative Study: 1993 Aug;15(4):227-35
Abstract: The use of nuclear grade as a prognostic indicator in breast cancer has been limited by its poor interobserver reproducibility. Automated cell classification using digital image analysis is one approach to this problem. - D B Chang, P C Yang, K J Chang, K T Luh, S H Kuo
Comparison of DNA stemline and cell kinetics between primary breast cancer and its lymph node metastasis
Comparative Study: 1993 Feb;15(1):32-8
Abstract: Quantitative measurement of nuclear DNA in individual cells was performed on imprint smears of surgical specimens from 25 primary breast cancers and their concurrent regional lymph node metastases (LNMs). - J M Dangou, R Kiss, C DePrez, M C Jeannot, R Fastrez, J L Pasteels, A Verhest
Heterogeneity of DNA ploidy, proliferation index and nuclear size in human colorectal carcinomas
1993 Feb;15(1):23-31
Abstract: Measurements of DNA ploidy, proliferation index and nuclear area were performed on 210 samples taken from 15 human colorectal tissues. The tissues were divided into four groups labeled G1, G2, G3 and C. - P Lipponen, S Aaltomaa, K Syrjänen
Morphometric quantitation of nucleolar organizer region proteins in breast carcinoma
1993 Oct;15(5):311-6
Abstract: Silver-stained proteins associated with nuclear organizer regions (Ag-NORs) were visualized using a standard silver staining technique in biopsy specimens from 130 breast carcinomas. - T R Burns, T A Teasdale, S D Greenberg
Use of morphometry as an aid in the differential diagnosis of large cell carcinoma of the lung
Comparative Study: 1993 Apr;15(2):101-6
Abstract: In a previous cytopathologic study a regression equation was generated that aided in the differential diagnosis of large cell carcinoma (LCC) of the lung from poorly differentiated adenocarcinoma (AC). This study was undertaken to validate and extend those findings. - A S Shabaik, J M Pow-Sang, J Lockhart, S V Nicosia
Role of DNA image cytometry in the follow-up of patients with urinary tract transitional cell carcinoma
Comparative Study: 1993 Apr;15(2):115-23
Abstract: Detection of recurrent urinary tract transitional cell carcinoma (TCC) is a frequent diagnostic challenge in exfoliative cytology because of the difficulty in distinguishing reactive changes from low grade tumors. - W H Wolberg, W N Street, O L Mangasarian
Breast cytology diagnosis with digital image analysis
1993 Dec;15(6):396-404
Abstract: An interactive computer system has been developed for evaluating cytologic features derived directly from a digital scan of breast fine needle aspirate slides. The system uses computer vision techniques to analyze cell nuclei and classifies them using an inductive method based on linear programming. - W H Wolberg, O L Mangasarian
Computer-designed expert systems for breast cytology diagnosis
Comparative Study: 1993 Feb;15(1):67-74
Abstract: Three expert systems have been developed to diagnose from nine scalar values visually assigned to epithelial cells obtained by breast fine needle aspiration. These expert systems achieved up to 0.98 sensitivity and 0.97 specificity. - J Klijanienko, A el-Naggar, F De Braud, C Micheau, F Janot, B Luboinski, A Gentile, A Russo, E Cvitkovic
Keratins 6, 13 and 19. Differential expression in squamous cell carcinoma of the head and neck
1993 Oct;15(5):335-40
Abstract: One hundred forty-one head and neck squamous cell carcinomas were analyzed for keratin (K) 6, 13 and 19. Staining was evaluated by light microscopy (with or without grading) and image analyzer and expressed as a percentage of positive versus all tumor surface (PSA). - R W Mackin, B Roysam, T J Holmes, J N Turner
Automated three-dimensional image analysis of thick and overlapped clusters in cytologic preparations. Application to cytologic smears
1993 Dec;15(6):405-17
Abstract: Methods are presented for automated analysis of thick and heavily overlapped regions of cytologic preparations, such as cervical/vaginal smears. Current systems are unable to process these regions although they contain diagnostically valuable information. - F A Clemo, W N Crabtree, E Walker, D B DeNicola
Comparison of image analysis and flow cytometric measurements of DNA content of canine transitional cell carcinomas
Comparative Study: 1993 Dec;15(6):418-26
Abstract: The DNA content of neoplasms is generally determined by flow cytometry, but quantitative microscopic image analysis is an alternative technique for DNA quantification. To compare these two methods, the DNA content of 31 paraffin-embedded canine transitional cell carcinomas was measured by flow cytometry and image analysis. - P Zeppa, A Vetrani, L Palombini, R Chiacchio, A Zabatta, I De Sio, L Castellano, M Calandra, F Fulciniti, G De Rosa
Evolution of DNA content in small and well-differentiated hepatocarcinoma
1993 Feb;15(1):12-22
Abstract: DNA ploidy of 10 well-differentiated hepatocarcinomas was evaluated by densitometry on smears obtained by fine needle aspiration biopsy (FNAB) with ultrasound guidance. Because of diagnostic difficulties or an increase in tumor size, FNAB was repeated once in six cases and twice in four. - R Christen, J Xiao, C Minimo, G Gibbons, B T Fitzpatrick, H Galera-Davidson, P H Bartels, M Bibbo
Chromatin texture features in hematoxylin and eosin-stained prostate tissue
1993 Dec;15(6):383-8
Abstract: A pilot study was undertaken to determine the expression of certain nuclear features in prostatic lesions. Twenty cases, 5 of hyperplasia and 5 each of carcinoma, Mostofi grades I-III, were selected as a training set, and an additional 20 cases were used as a test set, including 5 cases of hyperplasia and 5 cases each in Mostofi grades I-III. - S Munakata, J B Hendricks
Morphometric analysis of AgNORs in imprints and sections from non-Hodgkin's lymphomas. An approach to standardization
Comparative Study: 1993 Oct;15(5):329-34
Abstract: The argyrophilic nucleolar organizer region (AgNOR) technique was applied to tissue sections and touch imprints from 20 non-Hodgkin's lymphomas (NHL). The mean nuclear area (NA), mean AgNOR area/nucleus (AA) and NOR percentage nuclear area (NPNA) were determined using image analysis. - E M Wojcik, R L Katz, D A Johnston, D Sembera, A el-Naggar
Comparative analysis of DNA ploidy and proliferative index in fine needle aspirates of non-Hodgkin's lymphomas by image analysis and flow cytometry
Comparative Study: 1993 Jun;15(3):151-7
Abstract: To determine the application of image analysis (IA) to fine needle aspiration (FNA) of non-Hodgkin's lymphomas, 78 cases, categorized according to the Working Formulation, were analyzed and compared with those analyzed by flow cytometry (FCM). - R L Katz, E M Wojcik, A K el-Naggar, N G Ordonez, D A Johnston
Proliferation markers in non-Hodgkin's lymphoma. A comparative study between cytophotometric quantitation of Ki-67 and flow cytometric proliferation index on fine needle aspirates
Comparative Study: 1993 Jun;15(3):179-86
Abstract: Proliferation markers appear to predict biologic behavior and responses to therapy in non-Hodgkin's lymphoma (NHL). We examined the value of the antibody against Ki-67, a marker for cycling cells, in 73 fine needle aspirates and compared these results with parameters of ploidy. - A Berner, H E Danielsen, E O Pettersen, S D Fosså, A Reith, J M Nesland
DNA distribution in the prostate. Normal gland, benign and premalignant lesions, and subsequent adenocarcinomas
Comparative Study: 1993 Aug;15(4):247-52
Abstract: DNA content in paraffin-embedded tissue from 25 normal prostates (NP), 49 specimens of benign prostatic hyperplasia (BPH), 37 specimens of prostatic intraepithelial neoplasia (PIN) and 22 prostatic adenocarcinomas was measured by static image cytometry. - B M Ljung, D H Moore, F M Waldman, K L Chew, B H Mayall, H S Smith
Effects of short-term culture on benign and malignant human breast epithelium analyzed by image analysis
Comparative Study: 1993 Apr;15(2):107-14
Abstract: The ability to culture malignant breast cells is crucial to the success of many scientific studies. However, short-term cultures of breast cancers are composed predominantly of DNA diploid cells, leading to doubt regarding the presence of bona fide cancer cells in these cultures. - C L Gatlin, E S Schaberg, W H Jordan, B L Kuyatt, W C Smith
Point counting on the Macintosh. A semi-automated image analysis technique
1993 Oct;15(5):345-50
Abstract: In image analysis, point counting is used to estimate three-dimensional quantitative parameters from sets of measurements made on two-dimensional images. Point counting is normally conducted either by hand only or manually through a planimeter. - P D Unger, C W Watson, Z Liu, J Gil
Morphometric analysis of neoplastic renal aspirates and benign renal tissue
1993 Jun;15(3):206-12
Abstract: This study was designed to develop a practical morphometric procedure for the diagnosis of carcinoma in renal aspirates. We studied aspirates from renal cell carcinomas (grades 1-2) and normal kidneys. - D W Visscher, C Shaheen, S Drozdowicz, J D Crissman
Image cytophotometric DNA histogram heterogeneity in adenocarcinoma of the breast
1993 Jun;15(3):238-44
Abstract: In 36 breast carcinomas, DNA histograms derived from the edge of an invasive primary tumor were compared with those of a second area in the invasive tumor (n = 10) or of corresponding in situ (n = 22) and/or nodal metastatic (n = 6) components using image cytophotometry on formalin-fixed, paraffin-embedded sections. - F Hallouche, A E Adams, O R Hinton, D P Surtees, V Wadehra, G V Sherbet
Discriminant analysis for classification of murine melanomas and human cervical epithelial cells
1993 Feb;15(1):50-60
Abstract: Computer analysis of cell images offers many advantages over routine visual examination. It leads to quantitative and accurate detection of subvisual information and provides reproducible measures so that objective decisions in cancer diagnosis become possible. - T Schindewolf, W Stolz, R Albert, W Abmayr, H Harms
Classification of melanocytic lesions with color and texture analysis using digital image processing
1993 Feb;15(1):1-11
Abstract: The incidence of malignant melanoma, the most dangerous skin cancer, has increased rapidly during the last decade, and the figures are still rising. - M Auger, R L Katz, D A Johnston, N Sneige, N G Ordonez, H Fritsche
Quantitation of immunocytochemical estrogen and progesterone receptor content in fine needle aspirates of breast carcinoma using the SAMBA 4000 image analysis system
1993 Aug;15(4):274-80
Abstract: Evaluation of estrogen receptors (ER) and progesterone receptors (PR) in breast carcinoma using ER and PR immunocytochemical assays (ER-ICA and PR-ICA) has traditionally been semiquantitative at best. - H Bharucha, G McCluggage, J Lee, W Bannister, L Kuan, P Wilhelm, A Nelson
Grading cervical dysplasia with AgNORs using a semi-automated image analysis system
1993 Oct;15(5):323-8
Abstract: Colposcopic biopsies were classified according to previously established criteria by a group of three pathologists interested in cervical pathology. Ten cases were identified in each of the following five groups: - T Irinopoulou, J P Rigaut, M C Benson
Toward objective prognostic grading of prostatic carcinoma using image analysis
1993 Oct;15(5):341-4
Abstract: At present there is no generally accepted histopathologic grading system for prostatic carcinoma that would be reproducible, and it is still unclear which system provides the most reliable individual prognosis. - R J Sokol, G Hudson 2nd, J M Wales, D J Goldstein, N T James
Disordered macrophage development in Hodgkin's disease shown by quantitative cytochemistry
1993 Aug;15(4):253-8
Abstract: In order to investigate the disordered maturation of mononuclear phagocytes previously found in Hodgkin's disease, integrating microdensitometry was used to quantitate changes in seven cytochemical constituents (NADH dehydrogenase, succinate dehydrogenase, acid phosphatase, alpha-naphthyl butyrate esterase, DNA, RNA and glycogen) of developing macrophages from 19 patients and 19 normal subjects. - A Pogacnik, M Us-Krasovec, M Bracko
Preparation of fine needle aspiration biopsy samples for flow cytometric analysis
Clinical Trial: 1993 Aug;15(4):298-302
Abstract: In order to improve the quality of flow cytometric (FCM) DNA histograms, a new preparatory method was tested on samples obtained by fine needle aspiration biopsy (FNAB) of breast tumors. - E Artacho-Pérula, R Roldán-Villalobos, J Salas-Molina, R Vaamonde-Lemos
Histomorphometry of normal and abnormal cervical samples
Comparative Study: 1993 Aug;15(4):290-7
Abstract: We quantitatively studied the nuclear and cytoplasmic characteristics of cells in the normal cervix, squamous intraepithelial lesions (cervical intraepithelial neoplasia [CIN]) lesions and cervical human papillomavirus (HPV) infection with and without CIN (condyloma-CIN and condyloma-NCIN groups, respectively). - T K Lee, A L Wiley Jr, J D Esinhart, R S Riley, L D Blackburn
Variations associated with disaggregation methods in DNA flow cytometry
1993 Jun;15(3):195-200
Abstract: We investigated the variations in DNA ploidy by flow cytometry (FC) among cell suspensions acquired by different disaggregation methods from the same tumor specimens. - R Sennerstam, G Auer
Polyploidization by means of endoduplication in a human breast cancer cell line
1993 Oct;15(5):303-10
Abstract: Near DNA diploid human adult solid tumors are often associated with certain near-tetraploid cells. In an established human breast cancer cell line, Hs578T, with a DNA index in the hyperdiploid region, polyploid cells appeared during exponential growth. - C E Elson, D Kufe, W W Johnston
Immunohistochemical detection and significance of axillary lymph node micrometastases in breast carcinoma. A study of 97 cases
Comparative Study: 1993 Jun;15(3):171-8
Abstract: Paraffin blocks of all axillary lymph nodes from 97 patients with an initial histologic diagnosis of infiltrating ductal carcinoma and negative axillary nodes were recut and stained with two monoclonal antibodies, AE/AE3 (antikeratin) and DF3 (developed against breast cancer cells and reactive with a glycoprotein tumor-associated antigen). - F Willemse, M Nap, L B de Kok, H F Eggink
Image analysis in immunohistochemistry. Factors with a possible influence on the performance of VIDAS version 2.0, a commercially available true color image analysis system
1993 Apr;15(2):136-43
Abstract: This paper describes the evaluation of several factors with a possible influence on the performance of a commercially available image analysis system capable of true color image analysis. The software used by this system is VIDAS Version 2.0. - A Moragas, M García-Bonafé, I de Torres, M Sans
Textural analysis of lymphoid cells in serous effusions. A mathematical morphologic approach
1993 Jun;15(3):245-88
Abstract: Nuclear texture of reactive and well-differentiated neoplastic lymphocytes from serous effusions was studied by an approach based on principles of mathematical morphology. - J Grace, R Stankovic, L Gupta
Detection of aneuploidy and polyploidy in non-Hodgkin's lymphoma by computer video image analysis
Comparative Study: 1993 Aug;15(4):265-73
Abstract: Thirty-six cases of histologically intermediate and low grade non-Hodgkin's lymphoma, classified according to the International Working Formulation, were examined using image analysis cytometry. Aneuploidy was detected in 21 cases (58%). No significant difference in either the incidence or extent of aneuploidy was seen for cases that entered remission when compared to cases that did not. - G L Wied
Industrial developments in automated cytology as submitted by their developers
1993 Oct;15(5):358-70 - E A Jeckel-Neto, Y Ito, T Sato, H Tauchi
Quantitative immunohistochemistry of parvalbumin expression in the rat diaphragm
1993 Jun;15(3):201-5
Abstract: Parvalbumin is a calcium-binding protein involved in the mechanism of Ca++ exchange between sarcoplasmic reticulum and the myofibrils. This characteristic promotes a direct correlation between the amount of parvalbumin and the velocity of contraction-relaxation of skeletal muscle fibers. - D W Visscher, S Smilanetz, S Drozdowicz, S M Wykes
Prognostic significance of image morphometric microvessel enumeration in breast carcinoma
1993 Apr;15(2):88-92
Abstract: Using image morphometry, microvessel density was analyzed in 58 stage-heterogeneous breast carcinomas by counting vascular cross-sections in type IV collagen (basal lamina)-immunostained cryostat sections (methanol fixed, avidin-biotin method). - H X Bui, F Ballouk, A del Rosario, M Abdulla, M Khan, T A Jennings, J S Ross
Nuclear DNA content and clinical follow-up in resected pancreatic adenocarcinoma
1993 Dec;15(6):389-95
Abstract: Nuclear DNA content was determined by image analysis of paraffin-embedded tissue sections in 20 cases of resected pancreatic ductal adenocarcinoma. - K A Alanen, H Joensuu, P J Klemi
DNA content analysis of fine needle aspirates obtained from lymphatic tissue
1993 Dec;15(6):418-26
Abstract: DNA ploidy and S-phase fraction (SPF) of 69 lymphomas and 50 samples from benign lymphatic tissue were determined by flow cytometry from cells obtained by fine needle aspiration biopsy. An aneuploid histogram with a DNA index (DI) > 1.05 was obtained from 15 (22%) lymphomas. - P Guillaud, Z Wozniak, D Seigneurin
Simultaneous quantitation of DNA and nucleolar organizer regions by image cytometry
Comparative Study: 1993 Dec;15(6):418-26
Abstract: This paper describes a DNA/nucleolar organizer region-associated protein (NOR) double staining technique and quantitation method. On smeared cell slides, the acidic proteins associated with nucleolar organizer regions were revealed by the silver colloid technique, and DNA was stained with the fluorescent dye Hoechst 33342. - M García-Bonafé, A Moragas, I de Torres, M Sans
Fine needle aspiration diagnosis of renal oncocytoma. A mathematical morphologic approach
Comparative Study: 1993 Jun;15(3):213-9
Abstract: Differentiation between renal oncocytomas and well-differentiated oxyphilic cell renal carcinomas from fine needle aspiration cytology smears may present considerable difficulties. - J Smolle, R Hofmann-Wellenhof
Quantitative effects of intercellular signals on computer-simulated tumor patterns
1993 Jun;15(3):220-6
Abstract: The behavior of tumor cells within a particular in vivo microenvironment is considered to be regulated by intercellular signals. Previous studies of computer simulations of tumor growth demonstrated a qualitative effect of autocrine and paracrine signaling factors on the resulting morphologic patterns.