Volume 8 (1992) Issue 1 - Issue 6 | Analytical and quantitative cytology and histology

Volume 8 (1992) Issue 1 - Issue 6

A Morens, B Krief, G Brugal
The HOME microscope workstation. A new tool for cervical cancer screening
Review: 1992 Aug;14(4):289-94
Abstract: The Highly Optimized Microscope Environment (HOME) is a computerized microscope design for assisting pathologists and cytotechnologists in routine clinical tasks.
P H Bartels, D Thompson, J E Weber
Expert systems in histopathology. IV. The management of uncertainty
Review: 1992 Feb;14(1):1-13
Abstract: Expert systems deal with data that are categorical and conceptual, that represent elements of fuzzy sets and that often, by themselves, do not allow an unequivocal decision. The management of uncertainty in expert systems thus becomes a crucial issue.
J S Lee, W I Bannister, L C Kuan, P H Bartels, A C Nelson
A processing strategy for automated Papanicolaou smear screening
1992 Oct;14(5):415-25
Abstract: A multilayer processing strategy was developed for the automatic screening of conventionally prepared Papanicolaou smears. The processing stages include image segmentation, feature extraction, object classification and slide classification.
M L Hutchinson, L M Isenstein, J J Martin, D J Zahniser
Measurement of subvisual changes in cervical squamous metaplastic cells for detecting abnormality
1992 Aug;14(4):330-4
Abstract: Quantitative measures of visually normal squamous metaplastic cells exfoliated from the uterine cervix were obtained to test the hypothesis that these cells, like intermediate squamous and endocervical columnar cells, show subvisual evidence of atypia in cases of bonafide squamous intraepithelial neoplasia.
A Dejmek, C Strömberg, B Wikström, A Hjerpe
Prognostic importance of the DNA ploidy pattern in malignant mesothelioma of the pleura
Comparative Study: 1992 Jun;14(3):217-21
Abstract: Malignant mesotheliomas often raise a difficult diagnostic problem: once the diagnosis is made, the possibilities of predicting the biologic activity and prognosis of the tumor are limited. DNA ploidy patterns have been used as a prognostic instrument for other tumors, and this pattern was therefore studied in 37 cases of verified mesothelioma.
J G Bruno, G B Olson
Influence of cell cycle synchronization on digital image analysis of HL-60 granulopoiesis
1992 Feb;14(1):24-31
Abstract: Retinoic acid (RA)-treated HL-60 cells subjected to density arrest (DA) and double thymidine block (TB) synchronization demonstrated image feature changes associated with cellular proliferation and differentiation.
J M Orrell, S J Brett, J Ivanyi, G Coghill, A Grant, J S Beck
Measurement of the immunoperoxidase staining of macrophages within liver granulomata of mice infected with Mycobacterium tuberculosis
Comparative Study: 1992 Dec;14(6):451-8
Abstract: A quantitative image analysis technique developed for the measurement of the extent of macrophage activation and epithelioid cell differentiation was performed on mice infected experimentally with Mycobacterium tuberculosis.
K Preston Jr, R Siderits
New techniques for three-dimensional data analysis in histopathology
1992 Oct;14(5):398-406
Abstract: This paper describes two three-dimensional (3D) analytical techniques based on 3D mathematical morphology that have been found useful in quantifying the 3D spatial distribution of S-phase cells in a tubular tumor of the human breast.
T K Lee, J D Esinhart, L D Blackburn, J F Silverman
The size of small cell lung carcinoma cells. Ratio to lymphocytes and correlation with specimen size and crush artifact
1992 Feb;14(1):32-4
Abstract: The size of small cell lung carcinoma (SCLC) cells has often been ambiguously defined as one and a half to four times that of a lymphocyte.
S S Bacus, D M Chin, R K Stern, R Ortiz, S G Ruby, D S Weinberg
HER-2/neu oncogene expression, DNA ploidy and proliferation index in breast cancer
1992 Dec;14(6):433-45
Abstract: HER-2/neu gene expression, DNA ploidy and proliferation index were studied in 250 cases of breast cancer. Expression of HER-2/neu was determined by using an antibody to the HER-2/neu receptor. Ki-67 antibody was used to determine the proliferation index of the breast cancers, and the Feulgen method was used to assess DNA amounts in the tumor cells.
D S Schultz, R L Katz, S Patel, D Johnston, N G Ordonez
Comparison of visual and CAS-200 quantitation of immunocytochemical staining in breast carcinoma samples
Comparative Study: 1992 Feb;14(1):35-40
Abstract: Using the CAS-200 image analysis system, we compared the relationship between semiautomated computer measurements (QIC score) and visual scoring (H score) on 30 breast cancer fine needle aspirates (FNAs) and corresponding tissue specimens stained by the estrogen receptor immunocytochemical assay.
J Arrese Estrada, R Greimers, C Maguina-Vargas, G E Piérard
Nuclear planimetry and DNA flow cytometry of verruga peruana
1992 Aug;14(4):354-8
Abstract: Verruga peruana is a vascular hyperplasia that is sometimes histologically mistaken for a malignant neoplasm. An infiltrative pattern with nuclear pleomorphism and mitoses is prominent in some lesions.
X Pasteur, I Maubon, O Sabido, M Cottier, J L Laurent
Comparison of the chromatin stainability of human spermatozoa separated by discontinuous Percoll gradient centrifugation. A flow cytometric contribution
Comparative Study: 1992 Apr;14(2):96-104
Abstract: Many clinical programs in in vitro fertilization (IVF) use the classic Percoll sperm preparation technique to obtain a subpopulation of highly mobile spermatozoa.
P H Bartels, D Thompson, M Bibbo, J E Weber
Bayesian belief networks in quantitative histopathology
1992 Dec;14(6):459-73
Abstract: Bayesian belief networks have a dynamic range and numeric response characteristics that make them uniquely suitable for descriptive classification schemes.
R Montironi, L Diamanti, A Santinelli, M Scarpelli
Computer-aided S-phase fraction determination in DNA static cytometry in breast cancer. A preliminary methodologic study on cytologic material
Comparative Study: 1992 Oct;14(5):379-85
Abstract: This methodologic study was performed on a single-cell-cycle breast carcinoma to evaluate the feasibility of computer-aided S-phase fraction determination in DNA static cytometry.
G Wallin, M Bäckdahl, B Christensson, L Grimelius, G Auer
Nuclear protein content and Ki-67 immunoreactivity in nonneoplastic and neoplastic thyroid cells
1992 Aug;14(4):296-303
Abstract: The nuclear DNA content in thyroid tumor cells has been shown to be closely related to the malignant potential of the neoplasm. Besides DNA, nuclear protein (NP) constitutes the major mass of the nucleus. The NP content may vary significantly in relation to the proliferative stage in growing as compared to growth-arrested cells.
A Moragas, H Allende, M Sans, M T Vidal, M García-Bonafé
Mathematical morphologic analysis of liver cirrhosis. Correlation with etiology, clinical score and hepatocellular carcinoma
1992 Dec;14(6):483-90
Abstract: Remodeling of the cirrhotic liver was studied retrospectively by mathematical morphologic methods in 75 autopsy cases (40 alcoholic, 17 hepatitis B virus (HBV)-related and 18 cryptogenetic cirrhosis), including 28 hepatocellular carcinomas.
J D Seidman, J J Berman, G W Moore, R A Yetter
Multiparameter DNA flow cytometry of keratoacanthoma
1992 Apr;14(2):113-9
Abstract: Keratoacanthomas (KAs) are rapidly growing cutaneous lesions that frequently look much like well-differentiated squamous cell carcinomas (SCCs) but spontaneously regress. It is uncertain whether KA is a reactive hyperplastic lesion that mimics a neoplasm or a true (but defective) neoplasm that cannot sustain progressive growth.
G A Meijer, P J van Diest, J C Fleege, J P Baak
Syntactic structure analysis of the arrangement of nuclei in dysplastic epithelium of colorectal adenomatous polyps
1992 Dec;14(6):491-8
Abstract: In search of new objective classifiers of the degree of dysplasia in colorectal adenomatous polyps, we conducted syntactic structure analysis on hematoxylin-eosin-stained tissue sections of 59 colorectal adenomas.
R Zhou, D L Parker, E H Hammond
Quantitative peroxidase-antiperoxidase complex-substrate mass determination in tissue sections by a dual wavelength method
1992 Apr;14(2):73-80
Abstract: The aim of this study was to develop a method of quantitating prostate-specific acid phosphatase (PSAP) in histologic sections of prostate tumor tissue labeled with the peroxidase-antiperoxidase (PAP) complex technique using diaminobenzidine (DAB) as a substrate.
G Mariuzzi, A Santinelli, M Valli, S Sisti, R Montironi, L Mariuzzi, R Alberti, E Pisani
Cytometric evidence that cervical intraepithelial neoplasia I and II are dysplasias rather than true neoplasias. An image analysis study of factors involved in the progression of cervical lesions
1992 Apr;14(2):137-47
Abstract: Image analysis was performed on 40 Feulgen-stained histologic samples and 48 Feulgen-stained cytologic preparations representing normal squamous epithelium and all grades of cervical lesions (from mild dysplasia to invasive carcinoma) in order to characterize the evolutionary progressive changes in cervical epithelial proliferative disease toward malignancy.
J W Jukema, P J van Diest, F C de Waal, J P Baak
Prognostic value of morphometry in acute lymphoblastic leukemia of childhood
1992 Apr;14(2):120-8
Abstract: The prognostic value of morphometric features was studied in a group of 33 children with acute lymphoblastic leukemia (ALL) and compared with clinical and hematologic parameters.
J Guzman, A Rückmann, A Glaser, C Wittekind, B Schönfeld, G Kiefer
DNA cytophotometric analysis of breast cancer. Follow-up for 10 years
1992 Dec;14(6):427-32
Abstract: Forty-four cases of newly diagnosed invasive ductal breast cancer were studied by static cytometry at the time of diagnosis and were followed for 10 years to determine if the survival rate correlated with the nuclear DNA measurements.
A Moragas, H Allende, M Sans, M T Vidal, M García-Bonafé, P Huguet
Cirrhotic changes in livers from children undergoing transplantation. Image analysis
1992 Oct;14(5):359-66
Abstract: The pattern and extent of disorganization of the liver architecture were studied in 25 children undergoing orthotopic liver transplantation for cirrhosis.
S J Lockett, K Jacobson, B Herman
Quantitative precision of an automated, fluorescence-based image cytometer
1992 Jun;14(3):187-202
Abstract: Digital image-based cytometry of clinical specimens labeled with fluorescent, disease-specific markers holds promise for becoming an important diagnostic and prognostic technique because the technique can make a diverse range of quantitative biochemical, morphologic, densitometric and contextual measurements on intact specimens.
D Sun, S Biesterfeld, C P Adler, A Böcking
Prediction of recurrence in giant cell bone tumors by DNA cytometry
1992 Aug;14(4):341-6
Abstract: The most interesting therapeutic aspect of giant cell bone tumors is which patients can be cured without a risk of recurrence by intralesional surgery (curettage).
G Forsslund, A Kreicbergs, B Nilsson, A Zetterberg
Photographic densitometry for quantitative DNA analysis of cytologic and histologic specimens
1992 Apr;14(2):153-60
Abstract: We found that photographic densitometry (PD) is a useful technique for quantitative determinations of nuclear DNA content in clinical tumor material. Optimum conditions for the use of PD in clinical cytology and histopathology were worked out.
J J Meulman, P Zeppa, M E Boon, W J Rietveld
Prediction of various grades of cervical neoplasia on plastic-embedded cytobrush samples. Discriminant analysis with qualitative and quantitative predictors
1992 Feb;14(1):60-72
Abstract: The purpose of this study was to investigate whether discrimination into five groups of various grades of cervical preneoplasia and neoplasia is possible using discriminant analysis models.
A D Thomas, T Davies, A R Luxmoore
The Hough transform for locating cell nuclei
1992 Aug;14(4):347-53
Abstract: A method of cytologic image segmentation that works purely on the basis of geometric information is described. Cell nuclei are assumed to be circular or elliptical. The latest ellipse-finding Hough transform is then used to locate the nuclei. The results are encouraging.
I Salmon, R Kiss, B Franc, P Gasperin, R Heimann, J L Pasteels, A Verhest
Comparison of morphonuclear features in normal, benign and neoplastic thyroid tissue by digital cell image analysis
Comparative Study: 1992 Feb;14(1):47-54
Abstract: We analyzed the morphonuclear features of thyroid cell nuclei from 10 normal tissues (10 multinodular goiters), 10 benign tissues (10 adenomas) and 10 neoplastic tissues (10 carcinomas--2 follicular, 2 medullary and 6 papillary).
D F Cowan
Implementing a regulation-complaint quality improvement program on a commercial laboratory information system
1992 Oct;14(5):407-14
Abstract: Implementing a quality improvement (QI) program on an automated laboratory information system (LIS) in the current regulatory climate requires first that the QI program be defined and second that the selected LIS be able to capture important events and use flexible vendor-provided or user-defined routines to prepare reports.
T Okagaki, M Lau, D Zelterman
Information, discrimination and divergence in cytology. VI. Biases and errors of measurement in small samples
1992 Jun;14(3):238-44
Abstract: Total discrimination and total divergence have been shown to be useful measures of performance in diagnostic cytology.
M M Pinto
DNA analysis of malignant effusions. Comparison with cytologic diagnosis and carcinoembryonic antigen content
Comparative Study: 1992 Jun;14(3):222-6
Abstract: Twenty-three consecutive malignant effusions from 19 patients submitted for cytologic examination were analyzed for carcinoembryonic antigen (CEA) content and for DNA analysis by flow cytometry.
P H Bartels, D Thompson, J E Weber
Expert systems in histopathology. V. DS theory, certainty factors and possibility theory
1992 Jun;14(3):165-74
Abstract: Uncertainty management for the evaluation of evidence based on linguistic and conceptual data is taking advantage of developments in the Dempster-Shafer (DS) theory of evidence, possibility theory and fuzzy logic.
J Guzman, S Arbogast, K J Bross, R Finke, U Costabel
Effect of storage time of pleural effusions on immunocytochemical cell surface analysis of tumor cells
1992 Jun;14(3):203-9
Abstract: To determine how long tumor cells can be stored without losing their immunocytochemical reactivity, five malignant pleural effusions in EDTA-coated tubes were analyzed after different storage times at either 4 degrees C or room temperature.
M Koha, B Wikström, B Brismar
Colorectal carcinoma. DNA ploidy pattern and prognosis with reference to tumor DNA heterogeneity
1992 Oct;14(5):367-72
Abstract: Nuclear DNA content was measured in 72 colorectal carcinomas using single-cell microspectrophotometry on Feulgen-stained smears. Four samples were analyzed from each tumor. Patients were followed for 41-65 months (average, 53).
N Wang, B G Stenkvist, B Tribukait
Morphometry of nuclei of the normal and malignant prostate in relation to DNA ploidy
Comparative Study: 1992 Jun;14(3):210-6
Abstract: Fine needle biopsies from 70 patients with prostate carcinoma and 10 patients with benign hyperplasia were used to study area, variation in size and form factors of the nuclei by image analysis.
B Frigo, S Pilotti, D Coradini, G La Malfa, F Rilke
Estrogen and progesterone receptors in breast cancer. Immunohistochemical assay on scraping material
Comparative Study: 1992 Apr;14(2):129-36
Abstract: In order to demonstrate the reliability of immunocytochemical results on cytologic specimens for receptor analysis, the expression of estrogen and progesterone receptors was investigated using immunohistochemistry on frozen sections and on scraping material from the same samples of 50 breast carcinomas.
J R Krause, M K Blank
DNA content in fresh versus paraffin-embedded tissue. Flow cytometric analysis of 100 tumors
Comparative Study: 1992 Apr;14(2):89-95
Abstract: DNA ploidy analysis was determined on 100 consecutive tumors from a wide variety of sites using both fresh and paraffin-embedded tissue on the same specimen. The correlation coefficient (r) value between the methods was 0.85.
T Okagaki, M Lau, D C Brooker, D Zelterman
Morphologic scales and relative distances between lesions identified on Papanicolaou smears
Comparative Study: 1992 Aug;14(4):335-40
Abstract: We fit a Gaussian-type curve to a nonmonotonic transform of initial arbitrarily given scales (pseudoscalar transform) of a two-way discrete classification table by maximizing the likelihood (entropy) and computing morphologic scales of clusters of objects identified by visual judgments.
E Artacho-Pérula, R Roldán-Villalobos, A Roldán-Villalobos, J Salas-Molina, R Vaamonde-Lemos
Morphometry and discriminant analysis of the endometrium
1992 Aug;14(4):320-9
Abstract: The authors dated 100 normal endometrial biopsies. Only endometrial specimens with histologically confirmed subphases and no evidence of organic disease were accepted for study. The morphometry and stereologic measures of the glands, lumina and endometrial epithelium were assessed.
No authors listed
2nd International Conference on the Computerized Cytology and Histology Laboratory. Chicago, Illinois, March 8-11, 1992. Abstracts
1992 Jun;14(3):245-88
F Liautaud-Roger, J Dufer, M J Delisle, P Coninx
Thyroid neoplasms. Can we do any better with quantitative cytology?
1992 Oct;14(5):373-8
Abstract: The differential diagnosis of thyroid neoplasms by routine cytology presents major difficulties. We therefore looked for measurable nuclear parameters that could be generated from Feulgen-stained smears obtained by fine needle aspiration biopsy (FNAB).
D Briscoe, K Ni, G L Wied, A Montag, R T Miller, H E Dytch, M Bibbo
Comparison of estrogen receptor immunocytochemical assay in frozen and paraffin sections
Comparative Study: 1992 Apr;14(2):105-12
Abstract: Frozen and paraffin sections of 11 breast carcinomas were stained for estrogen receptors (ER) using the same rat monoclonal primary antibody, D75P3, as the marker and alkaline phosphatase as the chromogen-linking enzyme.
C Gottlieb, S Kini, M Feingold
Cytomorphometric analysis of small cell neoplasms of the lung from specimens obtained via bronchoscopy
1992 Feb;14(1):41-6
Abstract: Primary neoplasms of the lung composed of small cells consist of undifferentiated small cell carcinoma (UCS), carcinoid tumors (CT) and some non-small cell carcinomas (NonSC) that lack cytoplasmic differentiation.
O P Sangueza, D M Hyder, C R White Jr, J Pallander, M Perkins, N Remple, A Bakke
Comparison of image analysis with flow cytometry for DNA content analysis in pigmented lesions of the skin
Comparative Study: 1992 Feb;14(1):55-9
Abstract: The DNA ploidy of 85 melanocytic skin lesions was determined by flow cytometry (FCM) and interactive image analysis (IA) using nuclear extracts of paraffin-embedded tissue. Of the 85 lesions analyzed, 43 were malignant melanomas in different stages of evolution, 15 were dysplastic nevi, 11 were Spitz nevi, and 16 were other types of nevi.
H Martin, P Hufnagl, M Beil, K Wenzelides, J Gottschalk, W Rahn
Nucleolar organizer region-associated proteins in cancer cells. Quantitative investigations on gliomas, meningiomas, urinary bladder carcinomas and pleural lesions
Comparative Study: 1992 Aug;14(4):312-9
Abstract: Using a modified silver staining technique, we investigated nucleolar organizer region-associated proteins (AgNORs) in paraffin sections of 156 neoplastic tissues and other lesions, including gliomas (n = 41), meningiomas (n = 20), urinary bladder carcinomas (n = 58), and neoplastic and reactive lesions of the mesothelium of the pleural cavity (n = 37).
C P Tardif, A W Partin, B Qaqish, J P Epstein, J L Mohler
Comparison of nuclear shape in aspirated and histologic specimens of prostatic carcinoma
Comparative Study: 1992 Dec;14(6):474-82
Abstract: Nuclear shape analysis of histologic sections of operative specimens has separated patients with clinically localized prostatic carcinoma with good and poor prognoses. In order to become more useful clinically, nuclear shape should be evaluable preoperatively but is distorted by core biopsy.
W J Pieters, J Koudstaal, J J Ploem-Zaayer, J Janssens, J W Oosterhuis
The three-group metaphase as a morphologic indicator of high-ploidy cells in cervical intraepithelial neoplasia
1992 Jun;14(3):227-32
Abstract: There is a need for additional morphologic criteria to improve the value of histologic classification for the prediction of the biologic behavior of cervical intraepithelial neoplasia (CIN).
C Cohen, B F Walker, A R Solomon, P B DeRose
DNA ploidy and cell cycle analysis in ear malignant melanoma by flow and image cytometry
1992 Apr;14(2):81-8
Abstract: Sixteen ear malignant melanomas (MM) were studied for ploidy and cell cycle analysis by flow and image cytometry. The results were compared with clinical (age, sex, stage), histologic (depth of invasion, level, type) and prognostic (recurrence, death) parameters.
M Lau, T Okagaki
Information, discrimination and divergence in cytology. V. General symmetry of total discrimination and total divergence
1992 Jun;14(3):203-9
Abstract: Total discrimination and divergence are derived from Baye's theorem and based on backward (a posteriori) probabilities.
J C Kennedy, N el-Badawy, P B DeRose, C Cohen
Comparison of cell proliferation in breast carcinoma using image analysis (Ki-67) and flow cytometric systems
Comparative Study: 1992 Aug;14(4):304-11
Abstract: Cellular proliferation has been implicated as an important predictor of biologic behavior in breast cancer.
L Z Xu, L F Wang
Nucleolar organizer regions in aspirates of malignant lymphomas and benign disorders of the lymph nodes
Comparative Study: 1992 Apr;14(2):148-52
Abstract: Silver staining of nucleolar organizer regions (AgNOR) was used to differentiate malignant lymphoma and chronic lymphadenitis.
D Ploton, B Visseaux-Coletto, J C Canellas, C Bourzat, J J Adnet, C Lechki, N Bonnet
Semiautomatic quantification of silver-stained nucleolar organizer regions in tissue sections and cellular smears
1992 Feb;14(1):14-23
Abstract: Silver staining of nucleoli reveals argyrophilic proteins associated with nucleolar organizer region (Ag-NOR) proteins. Argyrophilic components appear as dots about 1 micron in diameter dispersed throughout the nucleolus (Ag-NOR dots).
C Lesty, C Chleq, G Contesso, C Jacquillat
Nucleoli and AgNOR proteins in 32 cases of primary breast carcinoma. Spatial pattern of interactions between 50 clinical and histometric criteria
1992 Jun;14(3):175-86
Abstract: The purpose of this paper is to analyze a method allowing selection of the best morphometric criterion for quantifying AgNOR proteins under conventional observation conditions by light microscopy.
P Sánchez, L Vidal, C Parrado, I Pérez de Vargas
Application of morphometric methods to the cytologic study of intradermal nevi
Comparative Study: 1992 Oct;14(5):386-90
Abstract: Twenty-one intradermal nevi were studied by morphometric methods in an attempt to morphologically characterize the two types of nevus cell--epithelioids, type A, and fusiforms, type C--and to quantify the differences between them.
R Nafe, V Kaloutsi, H Choritz, A Georgii
Elliptic Fourier analysis of megakaryocyte nuclei in chronic myeloproliferative disorders
1992 Oct;14(5):391-7
Abstract: Elliptic Fourier analysis was applied to megakaryocyte nuclei in bone marrow biopsies from 15 patients with chronic myelocytic leukemia with megakaryocyte predominance and from 15 patients with chronic megakaryocytic granulocytic myelosis.

Volume 8 (1992) Issue 1 - Issue 6

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