Volume 6 (1990) Issue 1 - Issue 6
- G L Wied
Selecting a computerized file system for cytopathology and histopathology laboratories
Editorial: 1990 Dec;12(6):373-8 - H E Dytch, G L Wied
Artificial neural networks and their use in quantitative pathology
Review: 1990 Dec;12(6):379-93
Abstract: A brief general introduction to artificial neural networks is presented, examining in detail the structure and operation of a prototype net developed for the solution of a simple pattern recognition problem in quantitative pathology. - G L Wied, H Dytch, M Bibbo, P H Bartels, D Thompson
Artificial intelligence-guided analysis of cytologic data
1990 Dec;12(6):417-28
Abstract: A design for the integration of artificial intelligence (AI) technology with large databases of clinical and objective cytologic data, such as are on file at the University of Chicago, is presented. - C A Rubio, I May
A method for quantitating the nuclear area of gastric polyps using image analysis
1990 Apr;12(2):117-21
Abstract: With the aid of an image quantifier, the nuclear area in Feulgen-stained sections from 29 gastric adenomas and 8 hyperplastic gastric polyps were recorded. - C A Rubio, E P Saraga, J Lindholm
Improved method for mapping gastric intestinal metaplasia using selective histochemical morphometry
Case Reports: 1990 Apr;12(2):122-6
Abstract: A gastrectomy specimen containing two tubular adenomas from a 67-year-old woman was mapped by an improved method using selective histochemical staining. - R De Vita, A Calugi, F Maggi, F Mauro, L Montevecchi, A Vecchione
Flow cytometric DNA analysis of the human cervix affected by human papillomavirus and/or intraepithelial neoplasia
1990 Oct;12(5):306-13
Abstract: The relative DNA contents of 164 cellular samples from 59 patients affected by the viral cytopathic effects (VCE) of human papillomavirus (HPV) infection and/or by cervical intraepithelial neoplasia (CIN) and 12 cellular samples from 12 normal donors were analyzed by flow cytometry (FCM) with the aim of correlating the cytometric measurements with the morphologic and etiologic parameters. - C MacAulay, B Palcic
An edge relocation segmentation algorithm
1990 Jun;12(3):165-71
Abstract: An automated procedure that refines the nuclear contour of a previously segmented nucleus is described. The algorithm makes use of intensity information, edge magnitude information and both object and edge connectivity information. - R Montironi, M Scarpelli, S Sisti, A Braccischi, P Gusella, E Pisani, R Alberti, G M Mariuzzi
Quantitative analysis of prostatic intraepithelial neoplasia on tissue sections
1990 Oct;12(5):366-72
Abstract: The changes in nuclear morphology (karyometry) and DNA content in prostatic intraepithelial neoplasia (PIN) were analyzed on tissue sections. The cases of PIN were subdivided into PIN 1 and PIN 2 based on the degree of proliferation and the anaplasia of the secretory cells lining the ducts and acini. - M Bibbo, D H Kim, H Galera-Davidson, C di Loreto, H E Dytch
Architectural, morphometric and photometric features and their relationship to the main subjective diagnostic clues in the grading of prostatic cancer
1990 Apr;12(2):85-90
Abstract: Novel software was developed to perform quantitative measurements of architectural and nuclear features in tissue sections. A pilot study was then undertaken to determine the diagnostic relevance of these quantitative features in prostatic tissue and the relationship of these objective features to the subjective clues used by practicing pathologists in the grading of prostatic adenocarcinoma. - J Thiele, S Wienhold, R Zankovich, R Fischer
A histomorphometric analysis of trephine biopsies of bone marrow from 65 patients with chronic myeloid leukemia. Classification of patients into subgroups with different survival patterns
1990 Apr;12(2):103-16
Abstract: A histomorphometric (planimetric) study was performed on trephine biopsies of the bone marrow taken at presentation from 65 patients (31 males and 34 females, with a median age of 48 years) with chronic myeloid leukemia (CML). - L Hart, R M Donovan, E Goldstein, F P Brady
Detection of human immunodeficiency virus in infected CEM cells using fluorescent DNA probes and a laser-based computerized image cytofluorometry system
1990 Apr;12(2):127-34
Abstract: Quantitative nonradioactive methods to measure the human immunodeficiency virus (HIV) in individually infected cells are needed for the direct assessment of HIV infection, for the evaluation of antiviral chemotherapy and for testing the efficacy of vaccines. - K H Plate, D Arndt, D Hellwig, J Rüschoff, H D Mennel
Assessment of histogenesis and proliferative potential in cytologic specimens of human brain tumors. Value of immunocytochemistry and nucleolar organizer regions
1990 Jun;12(3):157-64
Abstract: The value of immunocytochemistry and nucleolar organizer regions (NORs) for the histogenetic identification and the estimation of the proliferative potential of brain tumors was assessed by the investigation of imprint smears of 51 neurosurgical tumor specimens. - K Preston Jr, L M Firestone, B N Nathwani
Use of three-dimensional mathematical morphology in analyzing histologic images, with application to the subtyping of follicular lymphomas
1990 Dec;12(6):399-416
Abstract: Ongoing research on the differentiation between small-cell and large-cell follicular lymphomas ascertained that image analysis by three-dimensional morphologic transforms permits subtyping of follicular lymphomas with greater accuracy and reproducibility than can be accomplished visually by expert pathologists. - E Munck-Wikland, C A Rubio, G U Auer, R Kuylenstierna, J Lindholm
Control cells for image cytometric DNA analysis of esophageal tissue and the influence of preoperative treatment
1990 Aug;12(4):267-74
Abstract: The nuclear DNA content of 4,960 normal cells in 30 esophagectomy specimens was assessed by image cytometry. In relation to intermediate squamous epithelial cells, the mean transmission values were 25.6% higher for basal squamous epithelial cells, 10.1% higher for fibroblasts and 16.2% lower for lymphocytes. - R L Becker Jr, U V Mikel
Interrelation of formalin fixation, chromatin compactness and DNA values as measured by flow and image cytometry
Comparative Study: 1990 Oct;12(5):333-41
Abstract: The severity and consistency of the effect of formalin fixation on the quantitation of DNA by flow cytometry (FCM) and image cytometry (ICM) were studied. - R A Ambros, B R Pawel, I Meshcheryakov, J Kotrotsios, W C Lambert, R C Trost
Nuclear morphometry as a prognostic indicator in colorectal carcinoma resected for cure
1990 Jun;12(3):172-6
Abstract: The prognostic value of nuclear morphometry in addition to clinical and pathologic features was retrospectively studied in 64 cases of colorectal carcinoma resected for cure with a minimum of five years of follow-up. - M D Graham
Comparison of three hexagonal tessellations through extraction of blood cell geometric features
Comparative Study: 1990 Feb;12(1):56-72
Abstract: Although image data are almost universally acquired on rectangular sampling lattices, the regular hexagonal lattice offers important theoretical advantages for tessellation of images, particularly when subsequent processing involves operations on local image neighborhoods. - K Fukuda, T Iwasaka, T Hachisuga, H Sugimori, H Tsugitomi, F Mutoh
Immunocytochemical detection of S-phase cells in normal and neoplastic cervical epithelium by anti-BrdU monoclonal antibody
1990 Apr;12(2):135-8
Abstract: The use of a monoclonal antibody (MAb) to bromodeoxyuridine (BrdU) to identify S-phase cells was examined in 30 cervical biopsy specimens and 30 cervical smears from 18 patients with cervical neoplasia. - Y Uei, Y Noguchi
Comparison of cytologic markers for automated lung cancer screening
Comparative Study: 1990 Oct;12(5):327-32
Abstract: In a preliminary study for the development of an automated lung cancer cytology screening system utilizing both flow and image processing techniques, potential markers for the flow cytometric screening for carcinoma cells in sputum were analyzed. - M Bibbo, D H Kim, C di Loreto, H E Dytch, H Galera-Davidson, D Thompson, D L Richards, H G Bartels, P H Bartels
Tissue architectural features for the grading of prostatic carcinoma
1990 Aug;12(4):229-36
Abstract: In research for the development of a computer-aided workstation for the objective grading of prostatic carcinoma, tissue architectural (histometric) features were analyzed in ten cases each of well-differentiated, moderately differentiated and poorly differentiated carcinoma (as subjectively graded by the consensus of a panel of experts). - C Lesty, M Raphael, C Chleq, J L Binet
Nucleolar topography of nuclei in histologic sections. Application of a nonparametric approach to the study of breast cancer and non-Hodgkin's lymphoma
Comparative Study: 1990 Aug;12(4):242-50
Abstract: The position of the nucleolus within a near-convex nuclear profile was defined, without taking stereology into account, in terms of two distances, P and G, where P = pi p2/SN, G = pi 2/SN, p and g being, respectively, the smallest and greatest distances between the center of the nucleolus (considered as a disk) and the superficial nuclear membrane, and SN being the surface area of the nucleus. - K H Plate, J Ruschoff, H D Mennel
Nucleolar organizer regions in meningiomas. Correlation with histopathologic malignancy grading, DNA cytometry and clinical outcome
1990 Dec;12(6):429-38
Abstract: Eighty-one meningiomas (63 grade I, 9 grade II and 9 grade III) and 2 meningeal sarcomas (grade IV) were investigated by a simple one-step silver staining for nucleolar organizer region (NOR)-associated proteins (AgNOR technique) and by DNA cytometry. - D C Wilbur, M F Zakowski, C M Kosciol, D F Sojda, W T Pastuszak
DNA ploidy in breast lesions. A comparative study using two commercial image analysis systems and flow cytometry
Comparative Study: 1990 Feb;12(1):28-34
Abstract: DNA ploidy determinations on a series of 24 breast specimens were performed independently utilizing flow cytometry (FCM) and two separate commercially available computerized image analysis systems for image cytometry (ICM). - A Hjerpe, E Lindh, P Bistoletti, J George, D Groff
Use of cervical Cytobrush samples for dot-blot detection and Southern blot typing of human papillomaviruses using subgenomic probes
Comparative Study: 1990 Oct;12(5):299-305
Abstract: The use of Cytobrush samples for the detection and typing of cervical human papillomavirus (HPV) infections was analyzed. - J Thiele, S Wagner, D Dienemann, S Wienhold, R Fischer, H Stein
Megakaryocyte precursors (promegakaryoblasts and megakaryoblasts) in the normal human bone marrow. An immunohistochemical and morphometric study on routinely processed trephine biopsies
Comparative Study: 1990 Aug;12(4):285-9
Abstract: On routinely processed trephine biopsies of the normal human bone marrow derived from 15 patients, immunostaining with a monoclonal antibody against platelet glycoprotein IIIa (Y2/51) and morphometric measurements were performed for the determination of megakaryocyte precursor cells. - J I Epstein, W N Christensen, G D Steinberg, H B Carter
Comparison of DNA ploidy and nuclear size, shape and chromatin irregularity in tissue sections and smears of prostatic carcinoma
Comparative Study: 1990 Oct;12(5):352-8
Abstract: Image analysis measurements of nuclear size, shape, texture and DNA ploidy were compared in smears versus the corresponding 4-micron tissue sections, both prepared from radical prostatectomy specimens obtained from resections for prostatic cancer. - E Schulte, D Wittekind
Standardization of the Papanicolaou stain. I. A comparison of five nuclear stains
Comparative Study: 1990 Jun;12(3):149-56
Abstract: The staining characteristics of five nuclear stains used in a Papanicolaou staining procedure were investigated. - J C Stockert, P Del Castillo, A R Llorente, D M Rasskin, J B Romero, A Gómez
New fluorescence reactions in DNA cytochemistry. 1. Microscopic and spectroscopic studies on nonrigid fluorochromes
1990 Feb;12(1):1-10
Abstract: Some nonrigid DNA-binding antibiotics and fluorochromes that recognize adenine-thymine (AT) sequences are widely applied in biomedical research, but the microscopic use, spectral characteristics and DNA binding modes of other similar compounds have been overlooked or scarcely explored. - M Bibbo, P H Bartels, M Salguero, H E Dytch, E Lerma-Puertas, H Galera-Davidson
Karyometric marker features in fine needle aspirates of microinvasive follicular carcinoma of the thyroid
1990 Feb;12(1):42-7
Abstract: Karyometric measurements were performed on fine needle aspirates of clearly identifiable tumor areas and adjacent normal-appearing areas in the surgical specimens from ten patients with microinvasive follicular carcinoma of the thyroid. - P Del Castillo, A R Llorente, A Gómez, J Gosálvez, V J Goyanes, J C Stockert
New fluorescence reactions in DNA cytochemistry. 2. Microscopic and spectroscopic studies on fluorescent aluminum complexes
1990 Feb;12(1):11-20
Abstract: Metal-dye complexes are widely applied in light microscopic techniques for chromatin staining (e.g., hematoxylin and carmine), but fluorescent complexes between phosphate-binding cations and suitable ligands have been little used. - E Lerma-Puertas, H Galera-Davidson, P H Bartels, D H Kim, H E Dytch, M Bibbo
Karyometric marker features in fine needle aspirates of follicular adenoma of the thyroid
Comparative Study: 1990 Aug;12(4):223-8
Abstract: Karyometric measurements were performed on fine needle aspirates of clearly identifiable adenomatous areas and adjacent normal-appearing areas in the surgical specimens from ten patients with follicular adenomas of the thyroid. - M Fernö, B Baldetorp, M Akerman
Flow cytometric DNA ploidy analysis of soft tissue sarcomas. A comparative study of preoperative fine needle aspirates and postoperative fresh tissues and archival material
Comparative Study: 1990 Aug;12(4):251-8
Abstract: Flow cytometric (FCM) DNA ploidy measurements on frozen fresh samples of soft tissue sarcomas were compared with the corresponding analyses on preoperative fine needle aspirates and postoperative formalin-fixed archival tissues from the same tumors. - T K Lee, J F Silverman, R D Horner, C W Scarantino
Overlap of nuclear diameters in lung cancer cells
Comparative Study: 1990 Aug;12(4):275-8
Abstract: The nuclear diameters (NDs) of randomly selected malignant cells from 35 cases of small-cell lung cancer (SCLC; 4,370 nuclei) and 31 cases of non-SCLC (NSCLC; 1,280 nuclei) were measured on the pretreatment tissue sections by ocular micrometry. - R E Fausel, W Burleigh, D B Kaminsky
DNA quantification in colorectal carcinoma using flow and image analysis cytometry
Comparative Study: 1990 Feb;12(1):21-7
Abstract: Numerous studies using flow cytometry (FCM) have shown that DNA quantification and ploidy classification can provide information of prognostic significance for patients with colorectal carcinoma; recent advances in image analysis cytometry (image cytometry, ICM) provide a new, alternative technique for DNA quantification. - W H Wolberg, O L Mangasarian
Computer-aided diagnosis of breast aspirates via expert systems
1990 Dec;12(6):394-8
Abstract: Two computer-driven expert systems trained to correctly diagnose 369 fine needle aspirates of the breast on the basis of nine cytologic descriptive parameters were tested on 70 newly obtained aspirates (57 benign and 13 malignant). - C MacAulay, B Palcic
Fractal texture features based on optical density surface area. Use in image analysis of cervical cells
1990 Dec;12(6):439-46
Abstract: The spatial distribution of the optical density of an image of a cell nucleus can be imagined to resemble a rugged surface in a three-dimensional space. - L Sánchez, M Regh, S Biesterfeld, R Chatelain, A Böcking
Performance of a TV image analysis system as a microdensitometer
1990 Aug;12(4):279-84
Abstract: The performance of a TV image analysis system combined with an automated microscope (the Leitz TAS plus) as a microdensitometer and morphometric device was investigated. - S Abe, S Ogura, I Nakajima, S Makimura, Y Kawakami, K Inoue
Small-cell-type poorly differentiated squamous cell carcinoma of the lung. Cytologic, immunohistochemical and nuclear DNA content analysis
1990 Apr;12(2):73-7
Abstract: The relationship between the nuclear DNA content, the immunohistochemical findings, the clinical characteristics (tumor volume doubling time and survival) and the cytomorphologic features of small cell poorly differentiated squamous cell carcinoma of the lung was studied in ten cases. - D S Schultz, T Harry, K L Wong, M M Stilmant, D J Zahniser, M L Hutchinson
Computer-assisted grading of adenocarcinoma in prostatic aspirates
1990 Apr;12(2):91-7
Abstract: Conventional cytologic grading of fine needle aspirates of prostatic adenocarcinoma has been shown neither to be reproducible nor to correlate well with histologic grading. - T K Lee, R D Horner, J F Silverman, D V Jackson Jr, D Anderson-Goetz, C W Scarantino
Implications of nuclear diameter in small cell lung carcinoma
1990 Apr;12(2):78-84
Abstract: The nuclear diameter of 5,117 malignant cells from 42 small cell lung carcinoma (SCLC) patients was assessed either on pretreatment tissue sections (35 cases) or cytologic smears (7 cases) by ocular micrometry. - F Tezuka, I Sato, H Higashiizumi, N Endo, K Ito, M Kasai
Method for the quantitative evaluation of the distribution pattern of nuclei in normal and malignant endometrial epithelia
1990 Apr;12(2):146-8
Abstract: A quantitative method of evaluating the pattern of distribution of nuclei within a cell cluster was developed and applied to endometrial cytology. - D Turk, Z Singer, S Jukić, B Krstulović
New technique for processing paraffin-embedded tissue for Y chromosome fluorescence identification of trophoblastic disease
1990 Apr;12(2):146-8
Abstract: A new reprocessing technique for Y chromosome fluorescent body (q 12 region) detection of trophoblastic disease in previously paraffin-embedded tissues is described. - No authors listed
International Conference on the Computerized Cytology and Histopathology Laboratory. Chicago, Illinois, March 24-27, 1990. Abstracts
1990 Jun;12(3):182-221 - I Léger, F Giroud, G Brugal
Quantitative analysis of cytoskeletal proteins throughout the cell cycle of the MRC-5 fibroblastic cell line
1990 Oct;12(5):321-6
Abstract: Fluorescent dyes were used to stain actin, vimentin, tubulin and DNA in the same MRC-5 fibroblastic cells. Cytofluorometry and image analysis were then used to quantitatively evaluate the F actin, vimentin and tubulin content throughout the cell cycle. - M Koha, T O Caspersson, B Wikström, B Brismar
Heterogeneity of DNA distribution pattern in colorectal carcinoma. A microspectrophotometric study of fine needle aspirates
Comparative Study: 1990 Oct;12(5):348-51
Abstract: For 50 consecutive patients with colorectal carcinoma, the nuclear DNA content in four separate fine needle aspiration samples taken from the resected tumors was analyzed using single-cell Feulgen cytometry. - T Tsuji, H Sakai, Y Ohsaki, T Fujikane, T Shimizu, E Sakai, S Onodera
The effects of photosensitization by hematoporphyrin derivative on the protein content of cultured human lung cancer cells. A flow cytometric analysis
1990 Jun;12(3):177-80
Abstract: In order to study the cytotoxic mechanisms of photodynamic therapy (PDT), the changes in the intracellular content of protein and DNA of cultured human lung cancer cells were examined by flow cytometry using the fluorescent dyes fluorescein isothiocyanate (FITC) and propidium iodide (PI). - O Ferrer-Roca, E Ballester-Guardia, J A Martin-Rodriguez
Morphometric, densitometric and flow cytometric criteria for the automated classification of thyroid lesions
1990 Feb;12(1):48-55
Abstract: An automated classification of 73 thyroid lesions using a logical and mathematical approach was attempted. Densitometric, morphometric and flow cytometric parameters were used in Fisher linear discriminant functions to separate goiters or normal thyroids from adenomas and from carcinomas; the combination of this approach with binary discrimination improved the initial classification to a final efficiency of 81%. - F Gérard, S Guerret, J P Gérard, J A Grimaud
Usefulness of morphometry in the cytologic assessment of rectal tumors
1990 Jun;12(3):181 - E Artacho-Pérula, R Roldán-Villalobos, R Vaamonde-Lemos
Quantitative study of the microvascular pattern in tourniquet-induced muscle ischemia
1990 Apr;12(2):139-45
Abstract: Experiments were carried out on 40 adult Wistar rats to determine the effect of the application of a pneumatic tourniquet on the capillary network of the tibialis anterior muscles. The tourniquet was applied for four hours to the right hind limb above the knee. - I Ishiwata, C Ishiwata, M Soma, I Ono, T Nakaguchi, S Nozawa, H Ishikawa
Differences between cell lines of uterine cervical glassy cell carcinoma and large cell nonkeratinizing squamous cell carcinoma
Case Reports: 1990 Aug;12(4):290-8
Abstract: Cell lines were established from two uterine cervical cancers, a glassy cell carcinoma (GCC) and a large cell nonkeratinizing squamous cell carcinoma (LCSC), and studied by a variety of techniques, including histology, chromosome analysis, heterotransplantation and tumor marker analyses. - R L Teplitz, B B Butler, H Tesluk, B H Min, L A Russell, H M Jensen, L R Hill
Quantitative DNA patterns in human preneoplastic breast lesions
1990 Apr;12(2):98-102
Abstract: In 12 cases of human mammary carcinoma in which a preneoplastic atypical ductal hyperplasia was also identified, quantitative DNA (QDNA) measurements of thionine-stained samples from both lesions were performed using the Cell Image Analysis 100 system. - T Okagaki, D Zelterman
Information, discrimination and divergence in cytology. I. Theoretical aspects and definitions
1990 Oct;12(5):342-7
Abstract: The results of medical tests update the probability of diagnosis (diagnosability) of a patient from the population prevalence of a disease. - J M Peters, B J Miles, J J Kubus, J D Crissman
Prognostic significance of the nuclear DNA content in localized prostatic adenocarcinoma
1990 Oct;12(5):314-20
Abstract: The objective of this study was to determine if the nuclear DNA content could predict disease progression in patients with stage A or B prostatic cancer.