Volume 5 (1989) Issue 1 - Issue 6
- P H Bartels, J E Weber
Expert systems in histopathology. I. Introduction and overview
Review: 1989 Feb;11(1):1-7
Abstract: An introduction to, and overview of, expert systems is presented, along with some preliminary comments on their application in diagnostic and analytical histopathology and cytopathology. The terminology common to expert systems is defined, and the nature of expert systems is discussed. - P H Bartels, D Thompson
Expert systems in histopathology. III. Representation of knowledge as "structured objects"
Review: 1989 Dec;11(6):367-74
Abstract: In designing expert systems for histopathology, the representation of knowledge as "structured objects" may offer advantages over the use of a set of rules. - C M Payne, C G Bjore Jr, D W Cromey, F Roland
A comparative mathematical evaluation of contour irregularity using form factor and PERBAS, a new analytical shape factor
Review: 1989 Oct;11(5):341-52
Abstract: Contour irregularity was mathematically evaluated using two different analytical shape factors: (1) "form factor" (FF), a conventional type of shape analysis formula defined by the formula 4 pi A/P2, and (2) the "PErimeter Ratio Before and After Smoothing" (PERBAS). - A Böcking, R Chatelain
Diagnostic and prognostic value of DNA cytometry in gynecologic cytology
Review: 1989 Jun;11(3):177-86
Abstract: A survey of the diagnostic and prognostic value of DNA cytometric measurements in gynecologic tumors is given. In slight-to-moderate epithelial dysplasias of the uterine cervix, morphologic studies alone cannot make a definite distinction between benignity and malignancy, nor can they identify all precancerous lesions. - P H Bartels, H Hiessl
Expert systems in histopathology. II. Knowledge representation and rule-based systems
Review: 1989 Jun;11(3):147-53
Abstract: Two aspects of expert systems for use in diagnostic histopathology and cytopathology are examined: knowledge representation and the structure and operation of rule-based systems. - J Rotmensch, J L Schwartz, M A Beckett, D J Grdina, P Toohill, R R Weischelbaum
Characterization of a human ovarian carcinoma
Case Reports: 1989 Oct;11(5):324-30
Abstract: An epithelial ovarian carcinoma from a patient with progressive disease who had received combination chemotherapy was established as a cell line and characterized. The doubling time of the cell line was seven days. - D Larsimont, R Kiss, D d'Olne, Y de Launoit, W Mattheiem, R Paridaens, J L Pasteels, A Verhest
Relationship between computerized morphonuclear image analysis and histopathologic grading of breast cancer
Comparative Study: 1989 Dec;11(6):433-9
Abstract: A pilot study analyzed the relationship between several morphonuclear parameters and the Bloom-Richardson score for 37 invasive, not-otherwise-specified (NOS) ductal breast carcinomas. - A G Montag, P H Bartels, E Lerma-Puertas, H E Dytch, S Leelakusolvong, M Bibbo
Karyometric marker features in tissue adjacent to in situ cervical carcinomas
1989 Aug;11(4):275-80
Abstract: Subtle changes in nuclear chromatin structure have been documented in the histologically normal mucosa adjacent to neoplastic lesions. - W Jansen, A M van Ginneken, J P Baak, A W Smeulders
Construction of the diagnostic encyclopedia workstation. Computerizing pathology for the pathologist
1989 Dec;11(6):447-50
Abstract: The combination of a personal computer and a laser-vision disc player is an adroit tool for storing and retrieving textual information in combination with pictorial information. - A Böcking, R Chatelain, S Biesterfeld, E Noll, D Biesterfeld, D Wohltmann, C Goecke
DNA grading of malignancy in breast cancer. Prognostic validity, reproducibility and comparison with other classifications
1989 Apr;11(2):73-80
Abstract: The prognostic significance of the "DNA malignancy grade" (DNA-MG) was tested in a series of 104 breast cancer patients in comparison with TNM staging, histomorphologic grading according to Bloom and Richardson, mean nuclear area (MNA) and DNA-histogram classification according to Auer. - Y S Fu, L Cheng, I Huang, S Huang, E Wiesmeier, F Wettstein, M Weissman
DNA ploidy analysis of cervical condyloma and intraepithelial neoplasia in specimens obtained by punch biopsy
1989 Jun;11(3):187-95
Abstract: This study analyzed the feasibility of, and the strategy for, DNA ploidy analysis of cervical condyloma and intraepithelial neoplasia by a computerized digital imaging system. - H C Bauer, A Kreicbergs, C Silfverswärd, B Tribukait
Ploidy and morphology in osteosarcoma
1989 Apr;11(2):96-103
Abstract: In a cytometric DNA study of high-grade osteosarcoma, the relationship between DNA content and morphology was analyzed. The investigation, based on microspectrophotometry of tissue sections and flow cytometry (FCM), included both primary lesions and recurrences. - A Böcking, R Chatelain, A Salterberg, M Hagedorn, G Gross
Bowenoid papulosis. Classification as a low-grade in situ carcinoma of the epidermis on the basis of histomorphologic and DNA ploidy studies
Comparative Study: 1989 Dec;11(6):419-25
Abstract: The nature of bowenoid papulosis was investigated by a comparative investigation of 12 biopsy specimens of this lesion, 19 biopsy specimens of Bowen's disease, 14 biopsy specimens of squamous cell carcinoma and 10 biopsy specimens of seborrheic keratosis. - P Hufnagl, H Guski, G Wolf, K Wenzelides, H Martin, K Roth
The PARTICLE expert system for tumor grading by automated image analysis
1989 Dec;11(6):440-6
Abstract: While automated microscopic image analysis of histologic sections has been helpful in objectivizing histologic tumor grading and investigating the relationships between grading and prognosis, expert systems have the potential of linking image analysis and other data for statistical analysis and application to a wider range of tumors. - H Schimmelpenning, K Hamper, U G Falkmer, J Caselitz, G Seifert, G U Auer
Methodologic aspects of DNA assessment by means of image cytometry in tumors of the salivary glands. A comparison between the results obtained using sections and cytospin preparations from the same paraffin-embedded specimens
Comparative Study: 1989 Dec;11(6):379-83
Abstract: The image cytometric nuclear DNA assessments on paraffin-embedded tissue sections and on Cytospin preparations of disaggregated specimens from the same cases were compared in 98 salivary gland tumors, including 21 acinic cell carcinomas, 29 mucoepidermoid carcinomas, 21 adenocarcinomas and 27 adenoid cystic carcinomas. - H Galera-Davidson, C Pera-Madrazo, M Bibbo, F Rivera-Hueto, F Lopez-Rubio, H E Dytch
Cytometric and histologic predictors of prognosis in ampullary carcinoma treated with pancreatico-duodenectomy
1989 Aug;11(4):243-8
Abstract: The association between several clinical, histologic and karyometric variables and the survival rates of patients with ampullary carcinoma was examined. - E K Wong, E H Liang, E K Lin, D A Simmons, L G Koss
A selective mapping algorithm for computer analysis of voided urine cell images
1989 Jun;11(3):203-10
Abstract: One of the fundamental targets of the automated image analysis of cytologic preparations is the reduction of computer classification errors due to cells or other objects that do not lend themselves to image segmentation or that have morphologic features that may mislead the cell classification schemes. - F Michelassi, L E Vannucci, A G Montag, H E Dytch, M Bibbo
Nuclear morphometric measurements in rectal adenocarcinoma cells of patients of different races
1989 Jun;11(3):173-6
Abstract: The reasons for the different long-term prognoses of black and white patients following curative resection of a rectal adenocarcinoma are unknown. - F Kommoss, M Bibbo, M Colley, H E Dytch, W A Franklin, J A Holt, G L Wied
Assessment of hormone receptors in breast carcinoma by immunocytochemistry and image analysis. I. Progesterone receptors
1989 Oct;11(5):298-306
Abstract: Frozen sections of 30 breast carcinomas were stained for progesterone receptors (PRs) using a rat monoclonal primary antibody and an alkaline phosphatase-antialkaline phosphatase technique. - P C van Bodegom, J P Baak, C van Galen, R G Vanderschueren, S S Wagenaar
Quantitative pathologic analysis of first and second primary cancers in double tumors of the lung
Comparative Study: 1989 Feb;11(1):43-7
Abstract: In spite of the frequent occurrence of double tumors of the lung, pathologic reports on these tumors are rare. In this study, 34 patients with double tumors (10 metachronous and 24 synchronous) were quantitatively analyzed; in all cases, both the first and second tumors had been completely resected and had adequate archival material. - M Colley, F Kommoss, M Bibbo, H E Dytch, W A Franklin, J A Holt, G L Wied
Assessment of hormone receptors in breast carcinoma by immunocytochemistry and image analysis. II. Estrogen receptors
Comparative Study: 1989 Oct;11(5):307-14
Abstract: Frozen sections of 30 invasive breast carcinomas were stained for estrogen receptors (ERs) and the tumor cell proliferative rate by an immunoalkaline phosphatase technique. The stained sections were evaluated for ER by the microTICAS image analysis system. - C A Rubio, A Porwit-McDonald, M Rodensjö, A Duvander
A method of quantitating Paneth cell metaplasia of the stomach by image analysis
1989 Apr;11(2):115-8
Abstract: Paneth cells are one of the histologic components of intestinal metaplasia of the stomach, as are mucin-producing goblet cells. - G B Olson, C W Weber, C S Jolley, K M Olson
Computerized morphometric quantitation of cellular features in splenocytes from normal and nutrient-restricted mice
1989 Dec;11(6):407-12
Abstract: The morphometric analysis of splenocytes reveals quantitative changes in the chromatin and cytoplasm that may be used to distinguish between cells from normally fed animals and cells from subjects fed restricted diets. - A M Uyterlinde, A W Smeulders, J P Baak
Reproducibility and comparison of quantitative DNA histogram features obtained with a scanning microdensitometer and a flow cytometer in breast cancers
Comparative Study; 1989 Oct;11(5):353-60
Abstract: A pilot study compared DNA histogram features of ten invasive ductal breast cancers measured by three different techniques: in cytologic and histologic samples using a scanning densitometer and in suspensions of single nuclei using a flow cytometer. - D C Brown, K C Gatter, M S Dunnill, D Y Mason
Immunocytochemical analysis of cytocentrifuged fine needle aspirates. A study based on lung tumors aspirated in vitro
1989 Apr;11(2):140-5
Abstract: The value of Cytospin preparations of fine needle aspiration (FNA) biopsy material for immunocytochemical analysis was investigated using aspirates obtained from 23 resected human lung tumors. - P R Harnett, M L Greenberg, M H Tattersall, R F Kefford
Method of extracting DNA from fine needle aspirates of human solid tumors for Southern blot analysis
1989 Dec;11(6):375-8
Abstract: A rapid, simple, convenient method for extracting DNA from fine needle aspiration (FNA) samples of human solid tumors for Southern blot hybridization studies is described. After the preparation of an air-dried cytologic smear, the remaining sample in the needle was rinsed directly into a test tube for DNA extraction. - R J Zarbo, D W Visscher, J D Crissman
Two-color multiparametric method for flow cytometric DNA analysis of carcinomas using staining for cytokeratin and leukocyte-common antigen
1989 Dec;11(6):391-402
Abstract: Solid tumors contain heterogenous cell populations, resulting in flow cytometric (FCM) DNA quantitations of a mixture of tumor and host cells. - J E Weber, P H Bartels
Colonic lesion expert system. Evaluation of sensitivity
1989 Aug;11(4):249-54
Abstract: In a previous study, an expert system using visually assessed diagnostic clues for diagnosing colonic tissue as normal, adenoma or adenocarcinoma arrived at diagnoses agreeing with the evaluation by pathologists ("correct diagnoses") for all 49 cases of normal colon, for 49 of 50 cases of adenoma and for 48 of 49 cases of adenocarcinoma. - W Domagala, J Lasota, M Chosia, K Weber, M Osborn
Leukocyte-common antigen and vimentin are reliable adjuncts in the diagnosis of non-Hodgkin's lymphoma in fine needle aspirates
1989 Feb;11(1):15-21
Abstract: Alcohol-fixed fine needle aspirates of 82 non-Hodgkin's malignant lymphomas (NHLs) were tested for the presence of vimentin and leukocyte-common antigen (LCA) by means of monoclonal antibodies (MAbs) and indirect immunofluorescence. - A W Smeulders, A M van Ginneken
An analysis of pathology knowledge and decision making for the development of artificial intelligence-based consulting systems
1989 Jun;11(3):154-65
Abstract: This paper partly addresses the question "What artificial intelligence (AI) tools are appropriate for which parts of pathology?" by analyzing the structure and components of knowledge in pathology (e.g., observations plus archival and reference data) and which aspects of that knowledge should be expressible in an AI consulting system.
- C E Blomjous, A W Smeulders, J P Baak, W Vos, C M van Galen, C J Meijer
A comparative study in morphometric grading of transitional cell carcinoma of the urinary bladder
Comparative Study: 1989 Dec;11(6):426-32
Abstract: To overcome the considerable observer inconsistency in the histologic grading of transitional cell carcinomas, the value of four different morphometric grading methods was investigated in 61 tumors of the bladder. - A R Graham, C M Payne
Quantitative light and electron microscopic correlations of nuclear features in papillary thyroid and ovarian carcinomas
Comparative Study: 1989 Oct;11(5):331-40
Abstract: The light microscopic (LM) and electron microscopic (EM) features of nuclear bodies and nucleoli were quantitatively compared in ten papillary carcinomas each of thyroid (PC-Thy) and ovarian (PC-Ovar) origins, along with eight nonthyroid, nonovarian papillary neoplasms from other organs (PN-Oth). - C E Elson, S P Moore, W W Johnston
Morphologic and immunocytochemical studies of bronchioloalveolar carcinoma at Duke University Medical Center, 1968-1986
1989 Aug;11(4):261-74
Abstract: Between 1968 and 1986, the tumor registries at Duke University Medical Center and Durham VA Medical Center accumulated a total of 193 patients with a diagnosis of bronchioloalveolar carcinoma (BAC). - C MacAulay, H Tezcan, B Palcic
Adaptive color basis transformation. An aid in image segmentation
1989 Feb;11(1):53-8
Abstract: Multispectral images of stained cells enable the use of color differences to segment and/or to discriminate between image components, such as cell types and cellular subcomponents. - C Bergeron, P Shatz, R Margolese, P Major, A Ferenczy
Immunohistochemical studies with monoclonal antibodies B72.3 and MA5 on histologic and cytologic specimens from benign and malignant breast lesions
Comparative Study: 1989 Feb;11(1):33-42
Abstract: Monoclonal antibodies B72.3 and MA5 were tested by the avidin-biotin immunoperoxidase method in histologic sections of 38 benign, 22 precancerous and 22 cancerous breast lesions, as well as in fine needle aspiration (FNA) smears and cell blocks of 25 breast carcinomas. - E Schulte, D Wittekind
Standardized thionin-eosin stain in bronchial cytology. A substitute for hematoxylin-eosin Y staining
1989 Apr;11(2):131-9
Abstract: A standardized thionin-eosinic acid stain was developed as a quick and highly reproducible staining method for bronchial cytology. - G B Olson, C S Jolley, K M Olson, J G Bruno
Computerized morphometric differentiation of chromatin changes in splenocytes from normal and calorie-restricted mice
1989 Oct;11(5):361-6
Abstract: Morphometric image analysis of splenocytes obtained from mice fed calorie-restricted diets for periods of up to three weeks revealed slight but significant changes in nuclear size, nuclear texture and total amounts of stainable Feulgen-positive DNA as compared to age-and-sex-matched control cells. - W Domagala, J Lasota, K Weber, M Osborn
Endothelial cells help in the diagnosis of primary versus metastatic carcinoma of the liver in fine needle aspirates. An immunofluorescence study with vimentin and endothelial cell-specific antibodies
1989 Feb;11(1):8-14
Abstract: Fine needle aspirates of 5 primary hepatocellular carcinomas and 24 carcinomas metastatic to the liver were studied using vimentin and endothelial cell-specific monoclonal antibodies. - Y Kashimura, M Kashimura, A Horie
Cytologic, histologic, DNA ploidy and electron microscopic analyses of a case of vulvar Paget's disease
Case Reports: 1989 Dec;11(6):413-8
Abstract: The cells in a case of multifocal vulvar Paget's disease were studied by cytology, histology, ploidy analysis and electron microscopy. The Paget cells in smears were seen in an isolated or a sheetlike arrangement. - M L Hutchinson, D S Schultz, R A Stephenson, K L Wong, T Harry, D J Zahniser
Computerized microscopic analysis of prostatic fine needle aspirates. Comparison with breast aspirates
1989 Apr;11(2):105-10
Abstract: Computerized image analysis was employed to analyze fine needle aspiration smears of the prostate and breast using both high-resolution images of individual cells and medium-resolution images of scenes and clusters (contextual analysis). - P J van Diest, A W Smeulders, F B Thunnissen, J P Baak
Cytomorphometry. A methodologic study of preparation techniques, selection methods and sample sizes
1989 Aug;11(4):225-31
Abstract: The influence of methodologic aspects on cytomorphometric features was studied using preparations of hepatoma and/or mastocytoma cells. First, two preparation techniques (smear and oese) were compared. - E Rampoldi, L Larizza, S Heltai, E Erba, P Ubezio, V Schirrmacher
Cytologic and flow cytometric DNA analysis of multinucleated tumor cells and derived microcells
1989 Feb;11(1):59-66
Abstract: Microcell production by means of Colcemid-induced micronucleation and subsequent enucleation with the density gradient technique was adjusted for use with the murine T-lymphoma line ESb-M. - P Troncoso, H E Dytch, M Bibbo, G L Wied, P J Dawson
The significance of DNA measurements in a histologically defined subset of infiltrating ductal carcinomas of the breast with long-term follow-up
1989 Jun;11(3):166-72
Abstract: The nuclear DNA content and other karyometric parameters were evaluated in a histologically homogeneous group of invasive ductal carcinomas of the breast from 13 patients who survived 25 years after radical mastectomy and from 13 controls matched for histologic tumor grade, lymph node status, tumor size and patient age. - M Bibbo, A G Montag, E Lerma-Puertas, H E Dytch, S Leelakusolvong, P H Bartels
Karyometric marker features in tissue adjacent to invasive cervical carcinomas
1989 Aug;11(4):281-5
Abstract: A companion study showed the existence of statistically significant changes in the value of karyometric "marker features" in the nuclei of histologically normal-appearing ectocervical epithelium adjacent to carcinomas in situ. - R J Sokol, J Wales, G Hudson
Predictive value of discriminant analysis of monocyte ultrastructure in malignant lymphoma
1989 Dec;11(6):403-6
Abstract: Discriminant analysis was applied to morphometric data obtained from ultrastructural studies of blood monocytes from 20 normal subjects, 23 patients with Hodgkin's disease and 12 patients with non-Hodgkin's lymphoma. - A Böcking, R Chatelain, M Homge, R Daniel, A Gillissen, D Wohltmann
Representativity and reproducibility of DNA malignancy grading in different carcinomas
1989 Apr;11(2):81-6
Abstract: The reproducibility of the determination of the "DNA malignancy grade" (DNA-MG) was tested in 56 carcinomas of the colon, breast and lung while its representativity was tested on 195 slides from 65 tumors of the colon, breast and lung. - N G Link, B N Nathwani, K Preston Jr
Subclassification of follicular lymphomas by computerized image processing
1989 Apr;11(2):119-30
Abstract: Non-Hodgkin's lymphomas with a follicular pattern are subdivided on the basis of morphologic criteria into four subtypes. Each subtype has a distinctive natural history, response to therapy and survival, and precise histologic diagnosis is essential for optimal treatment. - U V Mikel, W F Engler, E Perez-Rosario, R L Becker, I W McLean
A comparative study of morphometric measurements of nucleoli in uveal melanomas from electron micrographs and plastic-embedded and paraffin-embedded sections
1989 Apr;11(2):111-4
Abstract: Morphometric measurements of nucleoli were done on uveal melanomas from surviving and nonsurviving patients. - J P Baak, E Noteboom, J J Koevoets
The influence of fixatives and other variations in tissue processing on nuclear morphometric features
1989 Aug;11(4):219-24
Abstract: The influence on the area and numerical density of nuclei was investigated in 5-mm-thick slices of guinea pig liver for different fixatives and variations in tissue processing: delay in fixation, air drying, degree of acidity of 10% formalin (= 4% formaldehyde), Bouin and mercury-formalin fixatives, acetone and ethanol dehydration and understretching and overstretching of the paraffin-embedded sections. - G J Rucklidge, A J Travis
An automatic objective estimation of vascularization of normal and tumor-invaded brain tissue using image analysis
1989 Aug;11(4):286-90
Abstract: A rapid method for the estimation of vascularization of normal and tumor-invaded brain tissue is described. - No authors listed
The 1988 Bethesda System for reporting cervical/vaginal cytologic diagnoses. Developed and approved at a National Cancer Institute Workshop, Bethesda, Maryland, U.S.A., December 12-13, 1988
1989 Oct;11(5):291-7
Abstract: The 1988 Bethesda System for reporting cervical/vaginal cytologic diagnoses was developed and approved at a National Cancer Institute Workshop, Bethesda, Maryland, U.S.A., December 12-13, 1988. - R Sennerstam, H Kato, G U Auer
Dissociation of cellular protein and DNA content in mild and moderate dysplasia as a reflection of the degree of aneuploidy in cancer
1989 Aug;11(4):255-60
Abstract: Tumor progression was analyzed in vivo. The bronchial epithelium in five beagle dogs was weekly treated by 20-methylcholanthrene (20-MC). Bronchial cells were harvested before each application of the drug. - R Eckhardt, G E Feichter, K Goerttler
Influence of the elimination of lymphocytes from tumor cell suspensions on the calculation of S-phase fractions by flow cytometry
1989 Dec;11(6):384-90
Abstract: Contaminating lymphocytes were eliminated from enzymatically obtained cell suspensions of 260 surgical biopsy specimens by density gradient centrifugation using lymphocyte separation medium (LSM) in an attempt to improve the determination of S-phase fractions by flow cytometry. - B W Killough, C A Nichols, J A Nicholson, T S Gansler
Diagnosis of pancreatic carcinoma by fine needle aspiration cytology and computerized cytomorphometry
1989 Aug;11(4):238-42
Abstract: Eighty-one fine needle aspirations (FNAs) of pancreatic masses were performed between 1980 and 1988. Histologic or clinical follow-up was available for correlation with 78 aspirates. The FNA cytologic diagnosis of pancreatic carcinoma had a sensitivity of 79% and a specificity of 91%. - J Grace, B Hall
Comparison of flow cytometric data obtained using fresh and paraffin-embedded lymphoid tissue
Comparative Study: 1989 Feb;11(1):67-71
Abstract: Flow cytometric (FCM) DNA analysis was carried out on 24 lymph nodes: 13 from benign reactive hyperplasias and 11 from non-Hodgkin's lymphomas. - T Izutsu, T Kagabu, I Nishiya
Diagnosis of benign and malignant cervical specimens by multifiberoptic microspectrophotometry and by flow cytometry
1989 Jun;11(3):196-202
Abstract: Two techniques for the automation of mass screening for cervical cancer were studied. Microspectrophotometry was tried first, using a novel multifiberoptic scanning system that measured the nuclear size and DNA content of cells in routine smears restained by the Feulgen technique. - J W Rippin, J A Woolgar
Analysis of cell cycle subpopulations from cytometric data
1989 Aug;11(4):232-7
Abstract: As a result of experimental error, measurements of the DNA content of cells from proliferating populations give rise to histograms in which the G1-phase, S-phase and G2-phase distributions overlap. - F Giroud, M P Montmasson
Reevaluation of optimal Feulgen reaction for automated cytology. Influence of fixatives
1989 Apr;11(2):87-95
Abstract: The Feulgen reaction is used for cytophotometric quantification of nuclear DNA and texture studies of chromatin structure. - G E Marti, V Zenger, N E Caproaso, M Brown, G C Washington, P Carter, G Schechter, P Noguchi
Antigenic expression of B-cell chronic lymphocytic leukemia lymphocytes
1989 Oct;11(5):315-23
Abstract: A flow cytometric analysis of lymphocytes in B-cell chronic lymphocytic leukemia (B-CLL) samples and in monocyte-depleted and T-cell-depleted normal peripheral blood (B-PBL) samples was undertaken using 129 reagents from the blind panel (BP) and 72 reagents from the cluster designation (CD) panel obtained from the Fourth International Leucocyte Differentiation Conference and Workshop, B-Cell Section. - T R Burns, R D Underwood, S D Greenberg, T A Teasdale, J Cartwright Jr
Cytomorphometry of large cell carcinoma of the lung
1989 Feb;11(1):48-52
Abstract: A computerized morphometry system was used to evaluate criteria for the cytologic diagnosis of large cell carcinoma (LCC) and poorly differentiated adenocarcinoma of the lung. - R Chatelain, A Willms, S Biesterfeld, W Auffermann, A Böcking
Automated Feulgen staining with a temperature-controlled staining machine
1989 Jun;11(3):211-7
Abstract: A Shandon Varistain 24-3 staining machine was modified in order to run automated DNA Feulgen staining. - H P Dinges, G Wirnsberger, H Höfler
Immunocytochemistry in cytology. Comparative evaluation of different techniques
Comparative Study: 1989 Feb;11(1):22-32
Abstract: Immunocytochemistry represents an important tool in diagnostic cytology. Its application, however, is of restricted value due to methodologic difficulties, leading in some cases to false-positive or false-negative results.