Volume 3 (1987) Issue 1 - Issue 6
- M Oberholzer, R A Ettlin, G Klöppel, M Löhr, M W Buser, M J Mihatsch, H Christen, U Marti, P U Heitz
Morphometry and immunocytochemistry
Review: 1987 May;9(2):123-32
Abstract: The use of morphometry (and stereology), especially in conjunction with immunocytochemistry, in surgical and experimental pathology is reviewed. - Y Collan, T Torkkeli, E Pesonen, E Jantunen, V M Kosma
Application of morphometry in tumor pathology
Review: 1987 May;9(2):79-88
Abstract: The application of morphometry in tumor pathology is discussed, including its use in studying tumor biology, classification, diagnosis, and histopathology. - J Wunderlich
Fluorescence flow cytometry and immunology applied to cancer
Review: 1987 May;9(2):133-7
Abstract: Antibodies against cell-surface determinants present on limited types of normal cells offer an approach to detecting and characterizing tumor cells suitable for fluorescence flow cytometry. - J P Baak
The principles and advances of quantitative pathology
Review: 1987 May;9(2):89-95
Abstract: Overview of quantitative pathology techniques, emphasizing interactive morphometry and its clinical applications. - G F Bahr, U V Mikel
Mass, volume, and dimensional distributions in biology, with special reference to cells
Review: 1987 Aug;9(4):341-54
Abstract: Examines the positively skewed distribution of mass in biology, concluding that all weights or masses can best be described by lognormal theory. - A V Loud
Electron microscopic morphometry
1987 Mar;9(1):7-12
Abstract: Outlines fundamental concepts of morphometry, correction factors for systematic errors, and efficient sampling principles for electron microscopy. - B Barlogie, P McLaughlin, R Alexanian
Characterization of hematologic malignancies by flow cytometry
Review: 1987 May;9(2):147-55
Abstract: Reviews the quantitative assessment of cellular DNA and RNA content by flow cytometry for diagnosing and prognosing hematologic malignancies. - J L Mohler, A W Partin, D S Coffey
Correlation of prognosis to nuclear roundness and to flow cytometric light scatter
Review: 1987 May;9(2):156-64
Abstract: Discusses the correlation between nuclear roundness and prognosis in prostatic carcinoma using quantitative pathology. - S H Ong, D Horne, C K Yeung, P Nickolls, T Cole
Development of an imaging flow cytometer
1987 Oct;9(5):375-82
Abstract: Describes the design and construction of a cell analyzer combining image cytometry and flow cytometry. - P Dörmer
Morphometry and cytometry correlated to quantitative autoradiography
Review: 1987 May;9(2):115-22
Abstract: Reviews studies correlating morphometric and functional parameters of individual cells. - N B Atkin
Cytophotometric DNA determination correlated to karyotype, particularly in cancer
Review: 1987 May;9(2):96-104
Abstract: Discusses DNA content variations in interphase tumor cells in relation to chromosomal abnormalities. - S H Paplanus, A R Graham
Morphometry in surgical pathology
1987 Oct;9(5):455-8
Abstract: Discusses the role of morphometry in identifying malignant cells in surgical pathology. - IR Wanless
The use of morphometry in the study of nodular and vascular lesions of the liver
1987 Mar;9(1):39-41
Abstract: Summarizes the use of morphometry on human autopsy livers to investigate hepatocellular nodules. - D L Rosenthal, V Manjikian
Techniques in the preparation of a monolayer of gynecologic cells for automated cytology
1987 Mar;9(1):55-9
Abstract: Discusses the techniques developed to prepare uterine cervical specimens for automated cytology screening. - R J Pelstring, S H Swerdlow
Improved nuclear contour indices for lymphoid morphometry
1987 Dec;9(6):469-74
Abstract: Examines nuclear shape factors for identifying clefted, cerebriform, or convoluted nuclei in lymphoid morphometry. - B H Federspiel, L H Sobin, E B Helwig, U V Mikel, G F Bahr
Morphometry and cytophotometric assessment of DNA in smooth-muscle tumors
1987 May;9(2):105-14
Abstract: Discusses challenges in predicting the behavior of GI smooth-muscle tumors. - I L Berryman, J M Harvey, G F Sterrett, J M Papadimitriou
The nuclear DNA content of human breast carcinoma
1987 Oct;9(5):429-34
Abstract: Analyzes nuclear DNA content in breast carcinoma specimens and its associations with clinical factors.
- C E Liedtke, T Gahm, F Kappei, B Aeikens
Segmentation of microscopic cell scenes
1987 Jun;9(3):197-211
Abstract: Different methods for the automated segmentation of microscopic cell scenes are presented with examples. - F M Benes, S W Matthysse, J Davidson, E D Bird
The spatial distribution of neurons and glia in human cortex based on the poisson distribution
1987 Dec;9(6):531-4
Abstract: A method was devised that employs deviations from the Poisson distribution to analyze the spatial arrangement of neurons and glia in human cerebral cortex. - H E Dytch, M Bibbo, P H Bartels, J H Puls, G L Wied
An interactive microcomputer-based system for the quantitative analysis of stratified tissue sections
1987 Mar;9(1):69-78
Abstract: This paper describes a microcomputer-based system that allows diagnostically relevant properties of stratified tissue sections to be objectively measured. - N Tanaka, T Ueno, H Ikeda, A Ishikawa, K Yamauchi, Y Okamoto, S Hosoi
CYBEST Model 4. Automated cytologic screening system for uterine cancer utilizing image analysis processing
1987 Oct;9(5):449-54
Abstract: CYBEST (Cyto-Biologic Electronic Screening System) utilizes image analysis technology for the automated prescreening of cervical cytology specimens. - F Spyratos, M Briffod, A Gentile, M Brunet, C Brault, A Desplaces
Flow cytometric study of DNA distribution in cytopunctures of benign and malignant breast lesions
1987 Dec;9(6):485-94
Abstract: One hundred seventy-eight cytopunctures of mammary lesions were obtained for cytologic diagnosis and flow cytometric (FCM) analysis of the nuclear DNA content. All lesions were excised and evaluated histologically; 106 were carcinomas and 72 were benign lesions. - D L Rosenthal, A Philippe, T L Hall, S Harami, N Missirlian, S C Suffin
Prognosis of moderate dysplasia. Predictive value of selected markers in routinely prepared cervical smears
1987 May;9(2):165-8
Abstract: Digital image analysis was used to extract features from 1,123 abnormal cells in 23 routinely prepared, Papanicolaou-stained cervical smears. All slides examined had a cytologic diagnosis of moderate dysplasia. - W Abmayr, E Mannweiler, D Oesterle, E Deml
Segmentation of scenes in tissue sections
1987 Jun;9(3):190-6
Abstract: The segmentation of scenes of fixed tissue sections for quantitative histopathology is the crucial step for further image processing. - R D Davenport, P E McKeever
Ploidy of endothelium in high-grade astrocytomas
1987 Mar;9(1):25-9
Abstract: To determine the ploidy and proliferative activity of the endothelium in high-grade astrocytomas, the nuclear DNA content of 11 high-grade astrocytomas, including two gliosarcomas, was measured by cytophotometry. - N J Pressman, J K Frost, P K Gupta, R L Showers, G W Gill, D L Cook, J K Frost Jr, R K Traub
A quantitative method for the detection and localization of quantum-limited events from radionuclides in cells and tissue sections by computer-enhanced video microscopy
1987 Aug;9(4):291-302
Abstract: Cellular dynamics often involve extremely low concentrations of biologically active substances, which can be radiolabeled and detected, localized and quantitated by autoradiography. The latter may require exposures from a few days to many months. - S D Greenberg, H J Spjut, R G Estrada, N R Hunter, C Grenia
Morphometric markers for the evaluation of preneoplastic lesions in the lung. Diagnostic evaluation by high-resolution image analysis of atypical cells in sputum specimens
1987 Mar;9(1):49-54
Abstract: A review is presented of the Baylor College of Medicine/NASA/Lyndon Johnson Space Center high-resolution image analysis system for the detection of preneoplastic lesions of the lung in sputum specimens. - K K Kayser, B Kiefer, H Toomes, H U Burkhard
Analysis of adenomatous structures in histopathology
1987 Jun;9(3):273-8
Abstract: A new idea of structure analysis in histopathology based upon first-order and third-order structures is presented. Networks formed by single cells and by tubulopapillary formations in adenomatous tissue were analyzed. - J E Weber, P H Bartels, H G Bartels, M Bibbo
Discrimination of DNA ploidy patterns by order statistics
1987 Mar;9(1):60-8
Abstract: The use of order statistics to discriminate and classify DNA ploidy patterns is proposed, especially for the classification of additional observations: whether a given sample is more likely to have come from a normal or an abnormal tissue, and with what probability, based on its ploidy pattern. - C Wittekind, E Schulte
Computerized morphometric image analysis of cytologic nuclear parameters in breast cancer
1987 Dec;9(6):480-4
Abstract: The value of nuclear morphometry in the preoperative fine needle aspiration (FNA) cytologic diagnosis of mammary lesions was investigated and correlated with the lymph node status of the patients. - G U Auer, U Askensten, K Erhardt, A Fallenius, A Zetterberg
Comparison between slide and flow cytophotometric DNA measurements in breast tumors
1987 May;9(2):138-46
Abstract: Nuclear DNA analysis was performed in 37 human mammary adenocarcinomas in order to elucidate the difficulties and pitfalls connected with the interpretation of DNA histograms obtained using different methodologic approaches. - O Sudilovsky, T K Hei
Prestaining of membrane markers to identify specific areas for Feulgen cytophotometric determinations in a single section
1987 Aug;9(4):323-7
Abstract: In cytospectrophotometric determinations of the nuclear DNA content in tissues, two consecutive sections are commonly employed: one stoichiometrically stained (as with the Feulgen reaction) for the actual measurements and a second routinely stained (as with hematoxylin and eosin) to define the limits of abnormal areas. - A Flint, J P McCoy Jr, T R Esch, A L Beckwith, G W Morley
Simultaneous measurement of DNA content and detection of surface antigens of cervicovaginal cells by flow cytometry
1987 Oct;9(5):419-24
Abstract: A method is described by which the measurement of the DNA content and the light scatter and the detection of a cervical carcinoma-associated antigen (CCA) of squamous epithelial cells can be simultaneously accomplished by flow cytometry (FCM). - L G Koss
Automated cytology and histology, A historical perspective
1987 Oct;9(5):369-74
Abstract: The current status of the techniques of image analysis, morphometry and DNA measurements of human cells and tissue samples is reviewed, and the goals of these various techniques, in terms of the detection, diagnosis and prognosis of human cancer, are briefly summarized. - P H Bartels, J E Weber, S H Paplanus, A R Graham
Detection of diagnostic clues in statistical histometry
1987 Aug;9(4):355-68
Abstract: Examples are given of the detection of diagnostic clues in quantitative cytology and histopathology by statistical testing, such as may be applied in image analytical procedures. - J P Baak, E C Wisse-Brekelmans, A M Uyterlinde, N W Schipper
Evaluation of the prognostic value of morphometric features and cellular DNA content in FIGO I ovarian cancer patients
1987 Aug;9(4):287-90
Abstract: Approximately 20% to 40% of the patients with a FIGO I ovarian tumor die within five years after the diagnosis. Morphologic studies (typing and grading) of the primary tumor are prognostically important, but poorly reproducible. - W A Franklin, M Bibbo, M I Doria, H E Dytch, J Toth, E DeSombre, G L Wied
Quantitation of estrogen receptor content and Ki-67 staining in breast carcinoma by the microTICAS image analysis system
1987 Aug;9(4):279-86
Abstract: The microTICAS image analysis system, originally designed for karyometry using Papanicolaou-stained or Feulgen-stained smears and tissue sections, has been adapted to assess tissue sections stained by immunohistochemical techniques.
- H Joensuu, P J Klemi, E Eerola
Diagnostic value of flow cytometric DNA determination combined with fine needle aspiration biopsy in thyroid tumors
1987 Aug;9(4):328-34
Abstract: The nuclear DNA content and the numbers of cells in the S and G2M phases of the cell cycle were determined by flow cytometry (FCM) in fine needle aspirates of 187 thyroid tumors to evaluate the diagnostic value of nuclear DNA content determination in combination with aspiration cytology. - T O Caspersson
History of the development of cytophotometry from 1935 to the present
1987 Mar;9(1):2-6 - P Strojny, Z Traczyk, M Rozycka, W Bem, W Sawicki
Fourier analysis of nuclear and cytoplasmic shape of blood lymphoid cells from healthy donors and chronic lymphocytic leukemia patients
Comparative Study: 1987 Dec;9(6):475-9
Abstract: The folding rates of the contours of nuclei and entire lymphoid cells were analyzed by Fourier analysis of the shapes. Smears of peripheral blood from healthy subjects and from patients with chronic lymphocytic leukemia (CLL: type B, stage zero) were routinely prepared and stained. - N Tanaka, T Ueno, H Ikeda, A Ishikawa, K Yamauchi, K Okamoto, S Hosoi
CYBEST-CDMS Model 2. Automated cell dispersion and monolayer smearing system for CYBEST
1987 Oct;9(5):445-8
Abstract: The CYBEST automated cell dispersion and monolayer smearing system (CDMS) employed an autosyringing device and a modified centrifuge to produce specimens with an adequate number of dispersed cells per square millimeter. - H F Nauth, G K Neumann, K D Feilen
Structural and morphometric analysis of parakeratotic and dyskeratotic cells exfoliated from various vulvar lesions. Correlation with data from cervical cytology
1987 Jun;9(3):243-52
Abstract: Smears were taken from the vulvar skin of 123 patients (21 with normal-appearing skin and 12 with dystrophic, 29 with benign, 20 with precancerous and 53 with malignant conditions). In each case, 20 nucleated horny squames with the highest visible degree of abnormality were identified in the smear. - I Näslund, C A Rubio, G U Auer
Nuclear DNA changes during pathogenesis of squamous carcinoma of the cervix in 3,4-benzopyrene-treated mice
1987 Oct;9(5):411-8
Abstract: The pathogenesis of squamous carcinoma of the uterine cervix was studied by means of morphologic and cytochemical techniques in mice treated with 3,4-benzopyrene. - Y S Fu, T L Hall, J S Berek, N F Hacker, J W Reagen
Prognostic significance of DNA ploidy and morphometric analyses of adenocarcinoma of the uterine cervix
1987 Mar;9(1):17-24
Abstract: In an effort to improve the prognostic accuracy of the histologic criteria used for cervical adenocarcinomas, the nuclear DNA ploidy levels, means and standard deviations of nuclear areas and amounts of lumen and neoplastic tissue were quantitated. - A Kriete, R Schäffer, H Harms, H M Aus
On-line transmission electron microscopic image analysis of chromatin texture for differentiation of thyroid gland tumors
1987 Jun;9(3):268-72
Abstract: Nuclei of the cells from the thyroid gland were analyzed in a transmission electron microscope by direct TV scanning and on-line image processing. The method uses the advantages of a visual-perception model to detect structures in noisy and low-contrast images. - S Abe, Y Tsuneta, S Makimura, K Itabashi, T Nagai, Y Kawakami
Nuclear DNA content as an indicator of chemosensitivity in small-cell carcinoma of the lung
1987 Oct;9(5):425-8
Abstract: The relationship between the nuclear DNA histogram pattern of tumor cells obtained by bronchoscopic brushing and the response to combination chemotherapy (Cytoxan + Adriamycin + Vincristine) was studied in 28 patients with small-cell carcinoma of the lung. - H J Kwikkel, T Timmers, M E Boon, M M van Rijswijk, J G Stolk
Relation of quantitative features of visually normal intermediate cells in cervical intraepithelial neoplasia I and II smears to progression or nonprogression of the lesion
1987 Oct;9(5):405-10
Abstract: The relationship between the quantitative features extracted from digitized images of visually normal intermediate cells in cervical intraepithelial neoplasia (CIN) I and II smears and the subsequent follow-up diagnosis was studied. - B Ljungberg, R Stenling, G Roos
Flow cytometric DNA analysis of renal-cell carcinoma. A study of fine needle aspiration biopsies in comparison with multiple surgical samples
Comparative Study: 1987 Dec;9(6):505-8
Abstract: The flow cytometric (FCM) DNA analysis of fine needle aspiration (FNA) biopsy material was compared with the DNA analysis of multiple surgical biopsy material from 44 renal-cell carcinomas. - N Wheeler, S C Suffin, T L Hall, D L Rosenthal
Prediction of cervical neoplasia diagnosis groups. Discriminant analysis on digitized cell images
1987 May;9(2):169-81
Abstract: The purpose of this study was to develop discriminant analysis models for predicting cervical dysplasia/neoplasia case diagnoses using cytometric features derived from the digital image analysis of cell monolayers. - S H Bigner, R Bjerkvig, O D Laerum, L H Muhlbaier, D D Bigner
DNA content and chromosomes in permanent cultured cell lines derived from malignant human gliomas
1987 Oct;9(5):435-44
Abstract: DNA content, as determined by flow cytometry (FCM), and ploidy, as determined planimetrically from chromosomes, were measured for 17 established human glioma-derived cell lines. - L G Koss, E M Eppich, K H Melder, R Wersto
DNA cytophotometry of voided urine sediment. Comparison with results of cytologic diagnosis and image analysis
Comparative Study: 1987 Oct;9(5):398-404
Abstract: Measurements of nuclear DNA were performed in urothelial cells in 54 Feulgen-restained cytocentrifuge preparations of voided urine previously studied visually and with an image analysis system. - S Abe, S Makimura, K Itabashi, Y Kawakami
Nuclear DNA content, cytomorphologic features and clinical characteristics of small cell carcinoma of the lung
1987 Dec;9(6):495-8
Abstract: The relationship between the cytomorphologic features, the nuclear DNA patterns and the clinical prognosis of patients with small cell carcinoma of the lung was studied. - K Erhardt, G U Auer
Mammary carcinoma. Comparison of nuclear DNA content from in situ and infiltrative components
Comparative Study: 1987 Jun;9(3):263-7
Abstract: Twenty-eight mammary carcinomas were analyzed with respect to their nuclear DNA content. Ten of the carcinomas were entirely in situ (noninfiltrative) while 18 showed areas of both infiltrative and noninfiltrative growth. - J Graham
Automation of routine clinical chromosome analysis. I. Karyotyping by machine
1987 Oct;9(5):383-90
Abstract: An automated karyotyping system suitable for widespread use in clinical laboratories is described. The software is implemented on a general-purpose, commercially available image analyzer (Magiscan 2) using TV input from a conventional research microscope with minimal modification. - C Gauvain, D Seigneurin, G Brugal
A quantitative analysis of the human bone marrow erythroblastic cell lineage using the SAMBA 200 cell image processor. I. The normal maturation sequence
1987 Jun;9(3):253-62
Abstract: A quantitative image analysis of the normal maturation sequence for the human bone marrow erythroblastic lineage was performed using the SAMBA 200 cell image processor. - R V Shack, P H Bartels, R A Buchroeder, R L Shoemaker, D W Hillman, D Vukobratovich
Design for a fast fluorescence laser scanning microscope
1987 Dec;9(6):509-20
Abstract: The design of a fast fluorescence laser scanning microscope is described and illustrated, with discussion of the design consideration of the principal components, including the optical elements. - A M van Driel-Kulker, W E Mesker, M J van der Burg, J S Ploem
Preparation of cells from paraffin-embedded tissue for cytometry and cytomorphologic evaluation
1987 Jun;9(3):225-31
Abstract: A method is described for the preparation of monolayer smears from paraffin-embedded tissue. The smears are suitable for automated image analysis and DNA measurements while still allowing interpretation of nuclear morphology.
- J W Gamel, I W McLean, R Greenberg
Statistical methods for the practical clinical application of morphometric measurements
1987 Mar;9(1):30-8.
Abstract: Four statistical methods are presented to determine the practical clinical value of measurements made from malignant tumors and to translate these measurements into a prediction of survival for each patient. - No authors listed
Proceedings of the International Symposium on Advances in Morphometry and Ploidy Determination. Washington, D.C., September 18-21, 1985
1987 May;9(2):79-187. - No authors listed
Proceedings of the International Conference on Advances in Morphometry and Ploidy Determination. Bethesda, Maryland, September 18-21, 1985
1987 Mar;9(1):1-78. - U V Mikel
Absolute DNA values from Feulgen microspectrophotometric measurements and quantitative electron microscopy. A comparison of two species
Comparative Study: 1987 Mar;9(1):13-6.
Abstract: Quantitative electron microscopy (QEM) and microspectrophotometry were used to correlate the Feulgen stain absorption values to the calculated picograms of DNA. Measurements were made in human lymphocytes, rainbow trout lymphocytes and nuclei of trout erythrocytes. - I Maubon, M Cottier, X Pasteur, M Morcelet, C Marsan, J L Laurent
Bronchial carcinoma and image analysis. Differentiation between poorly differentiated epidermoid carcinoma and small-cell carcinoma
1987 Dec;9(6):499-504.
Abstract: The utility of automated image analysis in the distinction between poorly differentiated epidermoid carcinoma (eight cases) and small-cell carcinoma (ten cases) was studied. Material obtained using the bronchial brushing technique was prepared by a cytocentrifugation technique. - D Schoëvaërt, C Cibert
Morphometric analysis of the cell outlines of normal and polyomavirus-transformed FR 3T3 fibroblasts
Comparative Study: 1987 Aug;9(4):315-22.
Abstract: A method is described for the analysis of cell shape, using an image analyzer connected to a computer to assess the cell outline. - A S Evans, J M Monaghan
The nuclear DNA content of vulvar skin using Feulgen microspectrophotometry and "four-quarter" analysis
1987 Jun;9(3):239-42.
Abstract: The nuclear DNA contents in subbasal (nonreplicating) cells of 161 vulvar biopsies from 31 patients were studied by Feulgen staining, integrated scanning microspectrophotometry (densitometry) and subsequent "four-quarter" analysis of the measurements. - O A Husain, K C Watts
Preparatory methods for DNA hydrolysis, cytochemistry, immunocytochemistry and ploidy analysis. Their application to automated and routine diagnostic cytopathology
1987 Jun;9(3):218-24.
Abstract: A review is presented of some methods used to prepare cytologic specimens for analytical and/or automated studies, with the steps of the procedures detailed in appendices. - J N Turner, D N Collins
Standard specimens for stain calibration and their application to the Papanicolaou stain
1987 Dec;9(6):521-30.
Abstract: Standardized specimens composed of extracts of biologic objects (nucleoprotamine and bovine liver) were developed as tools for the quantitative evaluation of stain performance on biologic substrates. - J Graham, D Pycock
Automation of routine clinical chromosome analysis. II. Metaphase finding
1987 Oct;9(5):391-7.
Abstract: Metaphase finding is an essential activity in chromosome analysis, and there is much to gain from its automation. This paper describes software for automatic metaphase finding developed for use as part of a routine clinical chromosome analysis system, principally for samples from blood and amniotic fluid. - E Johannisson, H Jacolino
Microfluorometric assessment of the DNA-DNP complex in human spermatozoa
1987 Jun;9(3):232-8.
Abstract: During spermiogenesis, the DNA-nucleoprotein complex undergoes alterations that are reflected in a decreasing capacity for binding DNA-specific dyes, such as ethidium bromide (EB). - J Arechaga, J Diaz, G F Bahr
Morphometry of nuclear rings at high electron microscopic resolution
1987 Aug;9(4):335-40.
Abstract: In studying the fine structure of the nuclear chromatin, a new organelle was discovered, the nuclear ring. In its isolated form, this structure is rather like a geometric torus: a smooth, structureless ring. - R E Duque, P A Ward
Quantitative assessment of neutrophil function by flow cytometry
1987 Mar;9(1):42-8.
Abstract: The use of flow cytometry (FCM) to quantitatively assess neutrophil function is reviewed. The methodology is capable of measuring a number of parameters involved in the oxidative pathways that form the basis of the activated neutrophil's contribution to the host defense mechanism. - M W Stevens, N L Fazzalari, D J Crisp
Quantitation of nuclear shape in non-Hodgkin's lymphoma. Alternate shape descriptors
1987 Dec;9(6):459-68.
Abstract: The nuclear contour index (NCI) has been used extensively to quantitate cellular shape in non-Hodgkin's lymphoma (NHL). - W Wöllmer
Application of a small microcomputer to cell image analysis
1987 Dec;9(6):535-9.
Abstract: Solutions to three problems in using small microcomputers for interactive cell image analysis are discussed.