Volume 20 (2004) Issue 1 - Issue 6
- Roberta Mazzucchelli, Francesca Barbisan, Lucia M Tarquini, Andrea B Galosi, Daniela Stramazzotti
Molecular mechanisms in prostate cancer. A review
Review: 2004 Jun;26(3):127-33
Abstract: Genetic studies have provided remarkable clues to the causes of prostate cancer (PCa). For example, in addition to the expected role of androgens in facilitating the development of PCa, the possibility that infections might lead to prostate cancer has been raised with the identification of RNASEL and MSR1 as familial prostate cancer genes; that insight will profoundly affect future studies and may ultimately lead to new approaches to the prevention of prostate cancer. - John P Ward, Vanesa Magar, Susan J Franks, Gabriel Landini
A mathematical model of the dynamics of odontogenic cyst growth
2004 Feb;26(1):39-46
Abstract: To formulate a mathematical model of odontogenic cyst growth and establish the dynamics of cyst enlargement and role of osmotic pressure forces throughout its growth. - Molly A Brewer, James Ranger-Moore, Mark H Greene, David S Alberts, Yun Liu, Hubert G Bartels, Amy C Baruch, Peter H Bartels
Preneoplastic changes in ovarian tissues
2004 Aug;26(4):207-16
Abstract: To test whether histologically normal epithelium within ovarian inclusion cysts and stroma exhibit changes in nuclear chromatin pattern that indicate the presence of occult ovarian lesions. - Caterina Vivani, Simona Magi, Roberta Mazzucchelli, Roberta Bacchiocchi, Alfredo Santinelli, Giovanni Muzzonigro, Francesco Blasi, Rodolfo Montironi, Francesca Fazioli
Immunohistochemical evaluation of urokinase plasminogen activator receptor in noninvasive and early invasive urothelial papillary neoplasia
2004 Feb;26(1):15-21
Abstract: To investigate urokinase plasminogen activator receptor (uPAR) expression in urothelial papilloma and in noninvasive and early invasive papillary carcinoma. - Riikka Mattila, Kalle Alanen, Stina Syrjänen
DNA content as a prognostic marker of oral lichen planus with a risk of cancer development
2004 Oct;26(5):278-84
Abstract: To assess the presence of aneuploidy in oral lichen planus (OLP) and its usefulness as a prognostic marker. - Marina Scarpelli, Rodolfo Montironi, Ugo Salvolini, Anna Messori, Peter H Bartels
Karyometric features of low and high grade glial tumors as compared with their MRI appearance
2004 Apr;26(2):87-96
Abstract: To compare the results of a magnetic resonance imaging (MRI) grading designed to identify low- and high-grade gliomas with karyometry used as a tool to grade primary brain tumors. - Gustavo Javier Meschino, Emilce Moler
Semiautomated image segmentation of bone marrow biopsies by texture features and mathematical morphology
Comparative Study: 2004 Feb;26(1):31-8
Abstract: To present the preliminary results of a method for semiautomated detection of fat and hematopoietic cells as well as trabecular surfaces in bone marrow biopsies in order to calculate the percentage of each type of tissue or cell area in relation to the whole area. - Edmond Kahn, Anne Vejux, Dominique Dumas, Thomas Montange, Frédérique Frouin, Vincent Robert, Jean-Marc Riedinger, Jean-François Stoltz, Philippe Gambert, Andrew Todd-Pokropek, Gérard Lizard
FRET multiphoton spectral imaging microscopy of 7-ketocholesterol and Nile Red in U937 monocytic cells loaded with 7-ketocholesterol
2004 Dec;26(6):304-13
Abstract: To show the effect of 7-ketocholesterol (7KC) on cellular lipid content by means of flow cytometry and the interaction of 7KC with Nile Red (NR) via ultraviolet fluorescence resonance energy transfer (FRET) excitation of NR on U937 monocytic cells by means of 2-photon excitation confocal laser scanning microscopy (CLSM). - Dariusz Wolowiec, Zdzisław Wozniak, Stanisław Potoczek, Grzegorz Ganczarski, Tomasz Wrobel, Kazimierz Kuliczkowski, Irena Frydecka, Michał Jeleń
Bone marrow angiogenesis and proliferation in B-cell chronic lymphocytic leukemia
2004 Oct;26(5):263-70
Abstract: To assess angiogenesis and the proliferative activity of bone marrow in patients with chronic lymphocytic leukemia (CLL) in relation to the bone marrow infiltration pattern. - Ricardo Losekann Paiva, Manoel Sant'Ana Filho, Paula Luce Bohrer, Isabel da Silva Lauxen, Pantelis Varvaki Rados
AgNOR quantification in cells of normal oral mucosa exposed to smoking and alcohol. A cytopathologic study
Clinical Trial: 2004 Jun;26(3):175-80
Abstract: To evaluate cell proliferative activity by counting and measuring argyrophilic nucleolar organizer regions (AgNORs) per nucleus in cell smears from mucosa clinically exposed to smoking and alcohol. - David Ramos, Amparo Ruiz, Luisa Morell, Samuel Navarro, Rafael Villamón, Manuel Gil-Salom, Antonio Llombart-Bosch
Prognostic value of morphometry in low grade papillary urothelial bladder neoplasms
2004 Oct;26(5):285-94
Abstract: To analyze the prognostic value of morphometry in low grade papillary urothelial bladder neoplasms (LGPUBNs). - Cláudio Tarta, Vinícius Duval da Silva, Cláudio Rolim Teixeira, João Carlos Prolla, Luise Meurer, Cesar Chiele Neto, Shinji Tanaka
Digital image analysis and stereology of angiogenesis in polypoid and nonpolypoid colorectal adenomas
2004 Aug;26(4):201-6
Abstract: To evaluate 3-dimensional parameters and bidimensional microvascular quantification in the different morphologic presentations of colorectal adenomas. - Vijay Kumar, Neelam Varma, Subhash Varma, Harpreet Vohra, Pankaj Malhotra, Usha Dutta, Suresh Chandra Sharma
Flow cytometric analysis of DNA indices, expression of p53 and multidrug resistance genes in multiple myeloma patients
2004 Oct;26(5):271-7
Abstract: To perform a quantitative analysis of DNA ploidy, S-phase fraction % (SPF%), p53 and multidrug resistance (MDR) gene expression as independent prognostic parameters and to compare these parameters with stage of the disease in multiple myeloma (MM) patients. - Pranab Dey, Logasundaram Rajesh
Fractal dimension in endometrial carcinoma
2004 Apr;26(2):113-6
Abstract: To mathematically assess in a pilot study, endometrial glandular margin irregularity in simple hyperplasia, complex atypical hyperplasia and well-differentiated endometrial carcinoma with the help of box counting of fractal dimension and to discriminate these lesions on the basis of box counting of fractal dimension of the gland. - Reinhold Nafe, Wolfgang Glienke, Renate Burgemeister, Rainer Gangnus, Beate Haar, Antje Pries, Wolfgang Schlote
Regional heterogeneity of EGFR gene amplification and nuclear morphology in glioblastomas. An investigation using laser microdissection and pressure catapulting
2004 Apr;26(2):65-76
Abstract: To study the regional heterogeneity of epidermal growth factor receptor (EGFR) gene amplification (EGFR-GA) in glioblastomas, considering the relationship between this mutation and morphology of tumor cell nuclei. - Michael Yoder, Robert L Zimmerman, Marluce Bibbo
Two-color immunostaining of liver fine needle aspiration biopsies with CD34 and carcinoembryonic antigen. Potential utilization in the diagnosis of primary hepatocellular carcinoma vs. metastatic tumor
2004 Apr;26(2):61-4
Abstract: To examine immunohistochemical staining of cell block material with antibodies against vascular marker CD34 and polyclonal carcinoembryonic antigen (pCEA) for their clinical utility as part of a 2-color staining protocol in fine needle aspiration (FNA) biopsy of liver masses to distinguish metastases from primary hepatocellular carcinoma (HCC). - Henrik Griesser, Heinz Sander, Ralf Hilfrich, Bettina Moser, Ulrich Schenck
Correlation of immunochemical detection of HPV L1 capsid protein in Pap smears with regression of high-risk HPV positive mild/moderate dysplasia
2004 Oct;26(5):241-5
Abstract: To immunostain Pap smears of high-risk (hr) HPV DNA-positive early squamous lesions for detecting HPV L1 protein. - Wendy A Wells, Charles P Daghlian, Tor D Tosteson, Margaret R Grove, Steven P Poplack, Sandra Knowlton-Soho, Keith D Paulsen
Analysis of the microvasculature and tissue type ratios in normal vs. benign and malignant breast tissue
Comparative Study: 2004 Jun;26(3):166-74
Abstract: To analyze the microvasculature and tissue type ratios in normal vs. benign and malignant breast tissue to establish a baseline for expected values against which future imaging studies can be benchmarked. - Neuza Kasumi Shirata, Luiz Marcelo Warneck Espoladore, Adhemar Longatto Filho, Janaína Erica Pittoli, Celso di Loreto, Sonia Maria Miranda Pereira, Marina Yoshie Sakamoto Maeda, Maria Lúcia Utagawa, Luciana da Silva Aguiar, Cecília Roteli-Martins, Edson G Martinez, Kari Syrjänen
High-risk HPV detection by Hybrid Capture and ploidy determination by a computer-assisted system in cervical biopsies
Clinical Trial: 2004 Jun;26(3):155-65
Abstract: To correlate high-risk HPV (hrHPV) detection by Hybrid Capture II (HC2) (Digene, Gaithersburg, Maryland, U.S.A.) with DNA content (ploidy) of cervical biopsies analyzed by a computer-assisted system. - James Ranger-Moore, Peter H Bartels, Paul Bozzo, Janine Einspahr, Yun Liu, Kathylynn Saboda, David S Alberts
Karyometric analysis of actinic damage in unexposed and sun-exposed skin and in actinic keratoses in untreated individuals
Clinical Trial: 2004 Jun;26(3):134-40
Abstract: To assess the ability of karyometric analysis to demonstrate progression of actinic damage as a function of sun exposure in individuals with actinic keratoses (AKs) and to evaluate the stability of that assessment over a 3-month period. - Arnold-Jan Kruse, Einar Gudlaugsson, Tove Helliesen, Emiel A Janssen, Bianca van Diermen, Silje Sandvik, Jan P Baak
Evaluation of prospective, routine application of Ki-67 immunoquantitation in early CIN for assessment of short-term progression risk
Clinical Trial: 2004 Jun;26(3):134-40
Abstract: To prospectively validate, in early cervical intraepithelial neoplasia (CIN), routine assessment of a previously developed prognostic Ki-67 immunoquantitative progression-risk model. - Dimosthenis Miliaras, Styliani Papaemmanuil
Cell kinetics analysis in stage I endometrial carcinoma as determined by image cytometry and immunohistochemistry. Correlation with clinicopathologic characteristics
2004 Feb;26(1):7-14
Abstract: To test the significance of various proliferative indices in endometrial carcinoma (EC) since previous investigations failed to yield consistent results that would establish them as factors of clinical importance. - Gwo-Shu Wang, Yu-Chin Lee, Reury-Perng Perng
A novel method of sputum processing for cytologic diagnosis of lung cancer
2004 Jun;26(3):121-6
Abstract: To compare the diagnostic sensitivity and cytologic findings of the sputum-processing method with those of the traditional sputum smear method. - Morten Ladekarl
Choice of methodology for quantifying cancer structures in tissue sections. A comparison of 2- and 3-dimensional estimators of mitotic activity, cellularity and nuclear size in breast cancer
2004 Apr;26(2):97-104
Abstract: To analyze the relationships between a range of 2-(2D) and 3-dimensional (3D) estimators of important tumor features (mitotic activity, cellularity and nuclear size). - Dimitris Glotsos, Panagiota Spyridonos, Panagiotis Petalas, Dionisis Cavouras, Panagiota Ravazoula, Petroula-Arampatoni Dadioti, Ioanna Lekka, George Nikiforidis
Computer-based malignancy grading of astrocytomas employing a support vector machine classifier, the WHO grading system and the regular hematoxylin-eosin diagnostic staining procedure
2004 Apr;26(2):77-83
Abstract: To investigate and develop an automated technique for astrocytoma malignancy grading compatible with the clinical routine. - Petros Karakitsos, Tatiana Mona Megalopoulou, Abraham Pouliakis, Michalis Tzivras, Athanasios Archimandritis, Aspasia Kyroudes
Application of discriminant analysis and quantitative cytologic examination to gastric lesions
2004 Dec;26(6):314-22
Abstract: To investigate the potential value of morphometry and discriminant analysis for the classification of benign and malignant gastric cells and lesions. - Katherine A Rider, Lisa M Flick
Differentiation of bone and soft tissues in formalin-fixed, paraffin-embedded tissue by using methylene blue/acid fuchsin stain
2004 Oct;26(5):246-8
Abstract: To find a staining method for formalin-fixed, paraffin-embedded tissue that would distinguish bone from surrounding soft tissues, including muscle, periosteal tissue and bone marrow. - Zissis Vesoulis, Lakshmi Rajappannair, Linda Define, Jackie Beach, Zachary Vesoulis, Brian Schnell, Sally Myers
Quantitative image analysis of estrogen receptors in breast fine needle aspiration biopsies
Comparative Study: 2004 Dec;26(6):323-30
Abstract: To establish whether the results of quantitative evaluation of estrogen receptors (ERs) in cytologic fine needle aspiration (FNA) biopsies of the breast are comparable to the results obtained on excised breast tumors and therefore suitable for making a clinical decision on tamoxifen treatment in women who are not candidates for surgery. - Kirti Gupta, Bishan D Radotra, Ashru K Banerjee, Raje Nijhawan
Quantitation of angiogenesis and its correlation with vascular endothelial growth factor expression in astrocytic tumors
2004 Aug;26(4):223-9
Abstract: To quantitate tumor angiogenesis by establishing intratumoral microvessel density (IMD), to study vascular endothelial growth factor (VEGF) expression in different grades of astrocytomas and to correlate VEGF expression with tumor angiogenesis. - Maohuai Chen
Evaluation of applying Feulgen stain for DNA analysis on destained hematoxylin-eosin-stained cytologic smears
Comparative Study: 2004 Oct;26(5):255-8
Abstract: To evaluate the feasibility and reliability of DNA analysis performed on the original hematoxylin-eosin (HE)-stained cytologic specimens by destaining the slides and restaining with the Feulgen method. - Dimitris Glotsos, Panagiota Spyridonos, Dionisis Cavouras, Panagiota Ravazoula, Petroula-Arampatoni Dadioti, George Nikiforidis
Automated segmentation of routinely hematoxylin-eosin-stained microscopic images by combining support vector machine clustering and active contour models
2004 Dec;26(6):331-40
Abstract: To develop a method for the automated segmentation of images of routinely hematoxylin-eosin (H-E)-stained microscopic sections to guarantee correct results in computer-assisted microscopy. - Swaroop S Singh, Bahjat Qaqish, Jacqueline L Johnson, O Harris Ford 3rd, Julie F Foley, Susan J Maygarden, James L Mohler
Sampling strategy for prostate tissue microarrays for Ki-67 and androgen receptor biomarkers
2004 Aug;26(4):194-200
Abstract: To develop an optimal sampling strategy for tissue microarrays using automated digital analysis for androgen receptor (heterogeneous expression) and the cellular proliferation marker Ki-67 (homogeneous expression and evaluated by others using non-automated methods). - Sambit K Mohanty, Pranab Dey, Subhash C Saha
Mean nuclear volume in ovarian papillary serous cystadenocarcinoma
2004 Feb;26(1):28-30
Abstract: To measure mean nuclear volume (MNV), estimated by the stereologic intercept method, in ovarian serous carcinomas and to compare that between omentum-positive cases (cases with omental metastasis) and -negative cases. - Shivani Ohri, Pranab Dey, Raje Nijhawan
Fractal dimension in aspiration cytology smears of breast and cervical lesions
2004 Apr;26(2):109-12
Abstract: To standardize the automated measurement of fractal dimension on cytologic smears and compare the fractal dimension of benign and malignant breast cells and cervical lesions on cytologic material to evaluate its role in the discrimination of benign from malignant cells. - Logasundaram Rajesh, Sanjib Kumar Pattari, Gurjeewan Garewal, Pranab Dey, Radhika Srinivasan
Image morphometry of acute leukemias. Comparison between lymphoid and myeloid subtypes
Comparative Study: 2004 Feb;26(1):57-60
Abstract: To determine if image morphometry has any role in distinguishing blasts of acute lymphoblastic leukemia (ALL-L2) from those of acute myeloid leukemia (AML-M1) and (AML-M2). - Deepali Jain, Logasundaram Rajesh, Radhika Srinivasan, Pranab Dey, Firuza D Patel
Utility of mean nuclear volume in predicting disease-free survival in locally advanced invasive cervical squamous cell carcinoma
2004 Aug;26(4):230-2
Abstract: To evaluate mean nuclear volume (MNV) as a prognostic indicator in invasive squamous cell carcinoma of the uterine cervix. - Francisco A Garcia, James Ranger-Moore, Bel Barker, John Davis, Molly Brewer, Jonathan Lozevski, Sajeet Vinyak, Yun Liu, Jon Yemane, Kenneth D Hatch, David S Alberts, Hubert G Bartels, Peter H Bartels
Karyometric image analysis for intraepithelial and invasive cervical lesions
2004 Jun;26(3):141-50
Abstract: To derive an objective, numeric measure for the progression of intraepithelial and invasive squamous cell cervical lesions. - Ekaterini N Politi, Andreas C Lazaris, Despina Alexopoulou, Sofia Lambropoulou, Nikolaos Kavantzas, Helen Koutselini
Morphometric analysis of AgNORs in thin-layer, liquid-based liver specimens
2004 Aug;26(4):187-93
Abstract: To detect argyrophilic nucleolar organizer regions (AgNORs) in ThinPrep (Cytyc Corp., Boxborough, Massachusetts, U.S.A.) liver fine needle aspiration (FNA) specimens and to define the diagnostic value of their quantitative analysis in the evaluation of hepatic lesions. - Logasundaram Rajesh, Kusum Joshi, Veena Bhalla, Pranab Dey, Bishan Das Radotra, Raje Nijhawan
Correlation between VEGF expression and angiogenesis in breast carcinoma
2004 Apr;26(2):105-8
Abstract: To analyze the role of vascular endothelial growth factor (VEGF) secreted by tumor cells in angiogenesis of breast carcinoma using image morphometry. - Peter H Bartels
In memoriam George L. Wied, M.D., D.Sc. (hon), F.I.A.C. February 7, 1921-July 25, 2004
2004 Dec;26(6):301-3
Abstract: In memoriam George L. Wied, M.D., D.Sc. (hon), F.I.A.C. February 7, 1921-July 25, 2004. - Lea Rath-Wolfson, Ilan Hammel, Valentina Kugel, Zvi Leib, Yoram Dekel, Rumelia Koren
Quantitative microscopy of human DNA topoisomerase II-alpha expression in transitional cell carcinoma of the bladder
2004 Aug;26(4):181-6
Abstract: To compare the nuclear size of various grades of transitional cell carcinoma (TCC) stained immunohistochemically with the nuclear enzyme topoisomerase II-alpha (topo II-alpha) in bladder urothelial neoplasms. - Koho Akimaru, Shigeki Yokomuro, Takayuki Aimoto, Hiroshi Yoshida, Eiji Uchida, Takashi Tajiri
Tumor marker measurements of cells in a fine needle used for aspiration cytology
2004 Oct;26(5):249-54
Abstract: To investigate whether the needle washing could yield sufficient cells for tumor marker (TM) measurements as an ancillary technique to ensure the accuracy of fine needle aspiration cytology (FNAC) of tumors. - Omer Günhan, Maria Kafousi, Bülent Kurt, Anastasios V Koutsopoulos, Yildirim Karslioğlu, Efstathios Stathopoulos, Bülent Celasun
Comparison of C-erbB2 (HER-2/Neu) immunohistochemistry results on invasive breast carcinomas. Experience of 2 Pathology departments in Turkey and Greece
Comparative Study: 2004 Jun;26(3):151-4
Abstract: To compare c-erbB2 (HER-2/Neu) immunohistochemical staining results obtained in 2 pathology departments and evaluate the reproducibility of staining and assessments. - Edmond Kahn, Anne Vejux, Gérard Lizard, Ginette Bessède, Frédérique Frouin, Céline Prunet, Jean-Claude Bernengo, Frédéric Brau, Andrew Todd-Pokropek, Philippe Gambert
Analysis of the fluorescence of monodansylcadaverine-positive cytoplasmic structures during 7-ketocholesterol-induced cell death
2004 Feb;26(1):47-56
Abstract: To analyze multilamellar cytoplasmic structures by confocal laser scanning microscopy (CLSM) combined with factor analysis of biomedical image sequences (FAMIS). - Koray Atalağ, Murat Sincan, Bülent Celasun, Ergun Karaağaoğlu
Effects of lossy image compression on quantitative image analysis of cell nuclei
2004 Feb;26(1):22-7
Abstract: To investigate whether statistically significant changes occur in quantitative image analysis of cell nuclei when lossy image compression techniques are used. - Edmond Kahn, Gérard Lizard, Dominique Dumas, Frédérique Frouin, Frank Ménétrier, Jean-François Stoltz, Andrew Todd-Pokropek
Analysis of fluorescent MRI contrast agent behavior in the liver and thoracic aorta of mice
2004 Aug;26(4):233-8
Abstract: To characterize the behavior of magnetofluorescent products injected in mice intravenously. - Edmond Kahn, Aline Receveur, Philippe Coullin, Frédérique Frouin, Catherine Blanco-Soulas, Andrew Todd-Pokropek, Alain Bernheim
Multiple excitation confocal analysis of targets in nuclei of cytogenetic preparations
Comparative Study: 2004 Feb;26(1):1-6
Abstract: To demonstrate that cellular preparations requiring color analysis of different domains stained by molecular cytogenetic methods (fluorescence in situ hybridization) can be processed by spectral analysis of fluorescent emissions by either factor analysis of medical image sequences (FAMIS) or a META confocal configuration to isolate fluorescent probes. - Yajue Huang, Robert L Zimmerman, Marluce Bibbo
Diagnostic value of CA 15-3 antibody in detecting metastatic adenocarcinoma
2004 Oct;26(5):259-62
Abstract: To determine the diagnostic value of CA 15-3 in detecting metastatic adenocarcinoma in body fluids using PreservCyt solution (Cytyc Corp., Boxborough, Massachusetts, U.S.A.) as collection fluid. - Sambit K Mohanty, Pranab Dey
Mean nuclear volume in squamous cell carcinoma of the vulva
2004 Apr;26(2):84-6
Abstract: To compare mean nuclear volume (MNV) estimated by the stereologic intercept method in lymph node-positive and -negative cases of squamous cell carcinoma of the vulva. - Georg W Herget, Wenko Smolka, Claus P Adler, Markus Uhl, Peter C Kreuz, Stefan M Knoller
Bone metastases: prognostic applications of cytometric DNA analysis
2004 Aug;26(4):217-22
Abstract: To examine DNA parameters as prognostic factors for developing metastases. - Pranab Dey, Logasundaram Rajesh
Glandular and stromal area in hyperplastic endometrium and carcinoma
2004 Apr;26(2):117-20
Abstract: To determine the role of image morphometry in distinguishing various follicular lesions of the thyroid in cytologic smears. - Logasundaram Rajesh, Mahamaya Saha, Srinivasan Radhika, Bishan Das Radotra, Arvind Rajwanshi
Morphometric image analysis of follicular lesions of the thyroid
2004 Apr;26(2):105-8
Abstract: To determine the role of image morphometry in distinguishing various follicular lesions of the thyroid in cytologic smears.