Volume 2 (1986) Issue 1 - Issue 6
- H H Callisen, M Pincu, A Norman
Feasibility of automating the micronucleus assay
1986 Sep;8(3):219-23
Abstract: The results of a feasibility study on the automation of the micronucleus assay in whole blood cultures of human lymphocytes are reported. - P C de Wilde, J P Baak, P J Slootweg, R J Hené, L Kater
Morphometry in the diagnosis of Sjögren's syndrome
1986 Mar;8(1):49-55
Abstract: Sublabial salivary gland biopsies of 20 patients with Sjögren's syndrome and 58 controls were analyzed morphometrically to determine which histologic changes in the tissue are specific enough to justify a diagnosis of Sjögren's syndrome and which changes are due to physiologic aging. - A B Sherman, L G Koss, D Wyschogrod, K H Melder, E M Eppich, C E Bales
Bladder cancer diagnosis by computer image analysis of cells in the sediment of voided urine using a video scanning system
1986 Sep;8(3):177-86
Abstract: A video-based computerized semiautomated image analysis system was applied to the diagnostic evaluation of 119 sediments of voided urine: 103 from patients with a broad variety of neoplastic and nonneoplastic disorders of the lower urinary tract and 16 normal controls. - A Böcking, W Auffermann
Algorithm for DNA-cytophotometric diagnosis and grading of malignancy
1986 Dec;8(4):363 - P A Coulombe, P R Filion, M G Côté
Biphasic mode of epithelial regeneration in murine pulmonary alveoli
1986 Sep;8(3):263-6
Abstract: After administration to mice of butylated hydroxytoluene, the pulmonary alveolar epithelium adopts a biphasic pattern of regenerative proliferation. This hitherto-unnoticed pattern of epithelial repair in the lung was revealed by the investigation of stereologic parameters. - M C Voisin, A Kemeny, Y Pinaudeau, J Villiaumey, D D Hioco
Variation of surface density and other parameters in remodeling of trabecular bone in various conditions
1986 Dec;8(4):333-7
Abstract: To appreciate the remodeling of the trabecular bone, the static morphometric parameters of surface density (SV(TRAB/BONE]) and volumetric fraction (VV(TRAB/BONE]) of cancellous bone were measured and compared to remodeling parameters, i.e., the surface extents of active/inactive resorption, active bone formation and nonmineralized bone. - M Oberholzer, W Remagen, R Orfei, R E Ehrsam, P U Heitz
Morphometry of bone. Are parameters from the iliac crest reliable indicators of renal-insufficiency osteopathy?
1986 Sep;8(3):250-4
Abstract: Bone samples from 25 autopsy cases with chronic renal insufficiency were analyzed stereologically. The findings in the iliac crest were found to be representative of those of the rib, femur condyle and lumbar vertebra for the following stereologic parameters. - P Ghidoni, E Giudici, L Matturri, L Rossi
Extranodal specialized sinoatrial cells
1986 Sep;8(3):255-8
Abstract: To define the occurrence of specialized pacemaker and transitional (slender) cells beyond the anatomic limits of the sinoatrial node, cytomorphometric evaluations were carried out in specimens from ten human hearts. - H Wehner
Urinary tract morphometry. An overview
1986 Dec;8(4):358-62
Abstract: An overview is presented of the application of morphometry to conditions in the urinary tract, with the work of a number of researchers briefly summarized. - J P Baak
Further evaluation of the practical applicability of nuclear morphometry for the prediction of the outcome of atypical endometrial hyperplasia
1986 Mar;8(1):46-8
Abstract: A previously developed morphometric classification rule has been shown to be successful in identifying approximately 30% of patients with atypical hyperplasia of the endometrium in whom the finding does not imply a progression to malignancy.
- H Galera-Davidson, M Bibbo, P H Bartels, H E Dytch, R Gonzalez-Campora, F Sanchez, G L Wied
Differential diagnosis between follicular adenoma and follicular carcinoma of the thyroid by marker features
1986 Sep;8(3):195-200
Abstract: Morphometric features descriptive of nuclear morphology, nuclear chromatin texture, and nuclear staining density were studied in tissues from encapsulated follicular carcinoma and follicular adenoma of the thyroid, whose differentiation is one of the most difficult tasks in thyroid pathology. - E Kahn, E Montès, F Bertin, G Contesso, R Di Paola
Evaluation of elastosis in breast cancer
1986 Sep;8(3):259-62
Abstract: The possibly prognostic value of elastosis in breast carcinoma has usually been visually evaluated, using subjective gradings whose variability has led to contradictory conclusions. - H J Kwikkel, M E Boon, M M van Rijswijk, W J Rietveld, J G Stolk
Masking effect of hormonal contraceptives on discriminating quantitative features of visually normal intermediate cells in positive and negative cervical smears
1986 Sep;8(3):227-32
Abstract: In quantitative studies of visually normal intermediate cells in smears from patients with cervical neoplasia, the contraceptive status of the patients has not previously been taken into account. - R J Sassen, J P Baak
Morphometry in the differential diagnosis of granulosa-cell tumors of the ovary
1986 Sep;8(3):245-9
Abstract: Although the diagnosis of granulosa-cell tumors of the ovary is usually consistent and reproducible, in some cases the differentiation from poorly differentiated adenocarcinomas can be difficult. - J P Baak, F A Langley, A Talerman, J F Delemarre
Interpathologist and intrapathologist disagreement in ovarian tumor grading and typing
1986 Dec;8(4):354-7
Abstract: In order to evaluate possible differences in the typing and grading of ovarian tumors, four different gynecologic pathologists independently evaluated slides from 198 patients. Histologic typing was done using the WHO recommendations, and the tumors were graded as benign, borderline, or malignant (well, moderately, or poorly differentiated). - J P Baak, J F Delemarre, F A Langley, A Talerman
Grading ovarian tumors. Evaluation of decision making by different pathologists
1986 Dec;8(4):349-53
Abstract: Although grading of ovarian tumors is widely performed, the criteria for each grade are not well defined; as a result, pathologists tend to establish their own criteria without, however, assessing the actual predictive value of the criteria. - E M Glaser, N T McMullen
Semiautomatic microscopy as a tool for cytologists
1986 Jun;8(2):116-27
Abstract: Despite impressive advances in the application of computer image analysis to cytology, many of the identification tasks that cytologists are called on to perform remain refractory to automated image analysis. - J M Baló-Banga, I Pfeiffer
Chromatin activation of blood lymphocytes detected by polarization microscopy and cytophotometry
1986 Mar;8(1):63-70
Abstract: Blood lymphocytes exhibit chromatin activation upon incubation with substances to which a person is allergic. Chromatin activation can be detected by polarization microscopy. - M Bibbo, P H Bartels, H Galera-Davidson, H E Dytch, G L Wied
Markers for malignancy in the nuclear texture of histologically normal tissue from patients with thyroid tumors
Comparative Study: 1986 Jun;8(2):168-76
Abstract: The chromatin of nuclei from histologically normal-appearing glands in tissue sections from patients with follicular adenoma, papillary carcinoma, and follicular carcinoma of the thyroid exhibited changes that were statistically significant when compared to the nuclear chromatin in tissue sections of the thyroid from normal individuals. - R G Knol, P C de Wilde, P J Slootweg
A BASIC program for computer-assisted point-counting techniques
1986 Mar;8(1):75-80
Abstract: Description is given of a BASIC program running on inexpensive microcomputers (Commodore CBM 64, SX 64, and Apple II[e]) to facilitate and accelerate substantially the point-counting technique, one of the most widely used methods in the determination of such stereologic parameters as VV, AA, SV, BA, JV, KV, and NV.
- J P Baak, G J Tan
The adjuvant prognostic value of nuclear morphometry in stage I malignant melanoma of the skin. A multivariate analysis
1986 Sep;8(3):241-4
Abstract: In order to evaluate the adjuvant prognostic value to tumor thickness of other clinical, qualitative histologic, and morphometric features, a multivariate analysis was performed in 68 patients with stage I cutaneous malignant melanoma and at least five years of follow-up. - B Reuter, U Schenck
Investigation of the visual cytoscreening of conventional gynecologic smears. II. Analysis of eye movement
1986 Sep;8(3):210-8
Abstract: The perception of visual information in cytoscreening was studied: eye movements were recorded while the cytotechnologist was screening cervical smears by means of a projection screen. Four phases of eye movement could be distinguished. - L Weiss, E Mayhew, P Ward
Drug delivery to tumors. A problem requiring microscopic resolution
1986 Jun;8(2):96-101
Abstract: One factor in the effectiveness of chemotherapy for both primary tumors and their metastases is the delivery of the drug to the cancer cells within the tumor. - P Tosi, C Miracco, P Luzi, M Cintorino, R Kraft, H Cottier
Morphometric distinction of granulomas in tuberculosis and sarcoidosis. Difference in nuclear profiles
1986 Sep;8(3):233-40
Abstract: Morphometric measurements were carried out on epithelioid-cell nuclei of noncaseating granulomas in paraffin-embedded sections of lymph node biopsy specimens originating from 15 patients with sarcoidosis and from 18 patients with tuberculosis. - D Seigneurin, G Brugal, D Payard, C Gauvain
A quantitative analysis of the human bone marrow granulocytic cell lineage using the SAMBA 200 cell image processor. II. The blast cells in refractory anemia with an excess of blasts
1986 Dec;8(4):281-92
Abstract: A quantitative image analysis of bone marrow blast cells from eight patients with refractory anemia with an excess of blast cells was performed using the SAMBA 200 cell image analyzer. - H E Dytch, M Bibbo, P H Bartels, G L Wied
Computer graphics in cytologic and pathologic microscopy. Tools for the clinician and researcher
1986 Jun;8(2):81-8
Abstract: Computer graphics capabilities are becoming practical economically and ergonomically for the first time on a widespread basis. - C Garbay, J M Chassery, G Brugal
An iterative region-growing process for cell image segmentation based on local color similarity and global shape criteria
1986 Mar;8(1):25-34
Abstract: An image segmentation process was derived from an image model that assumed that cell images represent objects having characteristic relationships, limited shape properties, and definite local color features. - F C Ramaekers, H L Beck, W F Feitz, P S Oud, F M Debruyne, G P Vooijs, C J Herman
Application of antibodies to intermediate filament proteins as tissue-specific probes in the flow cytometric analysis of complex tumors
1986 Dec;8(4):271-80
Abstract: The flow cytometric (FCM) analysis of carcinomas is often hampered by the presence of stromal and inflammatory cells in the cell suspensions obtained from such neoplasms. - B A Eccles, R R Klevecz
Automatic digital image analysis for identification of mitotic cells in synchronous mammalian cell cultures
1986 Jun;8(2):138-47
Abstract: Mitotic frequency in a synchronous culture of mammalian cells was determined fully automatically and in real-time using low-intensity phase-contrast microscopy and a newvicon video camera connected to an EyeCom III image processor. - H Galera-Davidson, M Bibbo, P H Bartels, H E Dytch, J H Puls, G L Wied
Correlation between automated DNA ploidy measurements of Hürthle-cell tumors and their histopathologic and clinical features
1986 Jun;8(2):158-67
Abstract: The treatment of Hürthle-cell tumors of the thyroid is controversial because of their rarity and the inconsistent histopathologic criteria for their diagnosis.
- J H Puls, M Bibbo, H E Dytch, P H Bartels, G L Wied
MicroTICAS. The design of an inexpensive video-based microphotometer/computer system for DNA ploidy studies
1986 Mar;8(1):1-7
Abstract: A description is presented of the microTICAS video-based microphotometer/computer system, which has been designed and implemented to provide an inexpensive means for the rapid assessment of DNA ploidy patterns. - R A Pearlstein, L Kirschner, J Simons, S Machell, W F White, R L Sidman
A multimodal system for reconstruction and quantification of neurologic structures
1986 Jun;8(2):108-15
Abstract: The utility of computers and computer graphics as aids in the study of nervous system architecture is growing. - R A Pearlstein, R L Sidman
Computer graphics presentation modes for biologic data. Types and examples
1986 Jun;8(2):89-95
Abstract: New advances in automated cytology, computer microscopy, densitometry, and other instrumentation for feature/scene acquisition or analysis have resulted in higher information densities than heretofore encountered. - H E Dytch, M Bibbo, J H Puls, P H Bartels, G L Wied
Software design for an inexpensive, practical, microcomputer-based DNA cytometry system
1986 Mar;8(1):8-18
Abstract: Recent advances in digital electronics have made it possible to put together videophotometry systems capable of practical DNA ploidy measurements at a relatively modest cost. - G Brugal, C Quirion, P Vassilakos
Detection of bladder cancers using a SAMBA 200 cell image processor
1986 Sep;8(3):187-94
Abstract: The cell image analysis of urinary sediments was performed using a SAMBA 200 system. Cell profiles were created using 18 parameters related to size, shape, densitometry, and chromatin texture. - R A Pearlstein
Toward an open architecture for morphometric computing
1986 Jun;8(2):128-30
Abstract: Prototype biomedical computing and graphics systems developed since the early 1970s for computer microscopy, architectonic or morphometric analysis, and visual display have been marked by the highly local character of their designs. - N M Maraldi, F Marinelli, R Ranaldi, S Papa, G M Mariuzzi, F A Manzoli
Morphometric and topologic analysis of freeze-fractured interphase nuclei
1986 Dec;8(4):343-8
Abstract: Interphase rat liver nuclei were studied by freeze fracturing followed by electron microscopic observations. This method permits information on the native organization of the nuclear components in the hydrated state to be obtained. - J L Salzmann, J B Michel, P Bruneval, M Ossondo Nlom, D R Barres, J P Camilleri
Automated image analysis of myocardial collagen pattern in pressure and volume overload in rat cardiac hypertrophy
1986 Dec;8(4):326-32
Abstract: An automated image analysis system was used to analyze the collagen pattern in picrosirius-stained sections of the left ventricular myocardium from 27 rats: 9 sham-operated animals (controls), 9 animals with one-clip, two-kidney Goldblatt renovascular hypertension (RVH), and 9 animals with an aortocaval fistula (ACF) model of volume overload hypertrophy. - A Göppinger, N Freudenberg, A Ross, H G Hillemans, M Hilgarth
The prognostic significance of the DNA distribution in squamous-cell carcinomas of the uterine cervix
1986 Jun;8(2):148-51
Abstract: To establish the prognostic parameters of DNA distributions of squamous-cell carcinomas of the uterine cervix, cervical smears from 30 patients were examined cytomorphometrically ten years after the diagnosis had been made. - B Stenkvist, E Bengtsson, O Eriksson, T Jarkrans, B Nordin
Image cytometry in malignancy grading of breast cancer. Results in a prospective study with seven years of follow-up
1986 Dec;8(4):293-300
Abstract: A fully automated system for image cytometry is presented. In combination with a special tumor sampling procedure, the system proved able to record with high reproducibility a large number of tumor cell population descriptors of high prognostic significance in a prospective study of breast cancer.
- G F Bahr, J C Lee
A chromosome's protein is a dependent variable of the total metaphase protein
1986 Sep;8(3):224-6
Abstract: Chinese hamster metaphases were stained with dansyl chloride to determine protein. Using a microfluorometer, the fluorescence of chromosome number one and the total of the fluorescence of the metaphase were measured. - H O Nielsen, B B Nielsen
Morphometric investigation of the human thyroid gland. With special reference to the methods and to nontoxic goiter
1986 Dec;8(4):338-42
Abstract: A study was carried out to (1) evaluate methods for the quantitative morphologic description of the human thyroid gland and (2) investigate the relationship between the morphology and the functional state of the gland in patients with nontoxic goiter. - H Schwarz
Morphometric requirements for image analysis and the IBAS interactive automatic image analysis system
1986 Sep;8(3):267-70
Abstract: The morphometric demands made on an image analysis system are discussed, as is the IBAS interactive automatic system, which meets those requirements. - G Godeau, G Gonnord, O Jolivet, D Schoevaert, A Pompidou, G Lagrue, A M Robert
A selective histochemical method for the quantitative estimation of elastic fibers by computerized morphometric analysis. Effect of colchicin treatment
1986 Dec;8(4):321-5
Abstract: A morphometric technique is reported that uses a new selective staining of the elastic system fibers in skin biopsy specimens to facilitate the quantitative evaluation of the volume fraction occupied by these elastic fibers in the tissue. - J Auger, D Schoevaert, E D Martin
Comparative study of automated morphometric and semiquantitative estimations of alcoholic liver steatosis
1986 Mar;8(1):56-62
Abstract: Needle biopsy of the liver is of great value in appreciating the intensity, type, and topography of the steatosis commonly observed during chronic alcoholic diseases. The usual semiquantitative optical analysis is very inaccurate and depends on the subjectivity and training of the pathologist. - U Schenck, B Reuter, P Vöhringer
Investigation of the visual cytoscreening of conventional gynecologic smears. I. Analysis of slide movement
1986 Mar;8(1):35-45
Abstract: In order to elucidate the process of visual cytoscreening, graphic and electronic recordings of the stage movements in visual screening of conventional gynecologic smears were carried out. More than 400 recordings have been evaluated. - M A Trambert
Cellular logic implementation on a generalized image processor applied to biomedical image processing
1986 Jun;8(2):131-7
Abstract: The implementation on a generalized image processor of a cellular logic package that performs non-recursive cellular-logic operations (CLOs) in real time is described. - L R Jarvis
A microcomputer system for video image analysis and diagnostic microdensitometry
1986 Sep;8(3):201-9
Abstract: A system for microdensitometry based on a microcomputer, video digitizer, and solid-state camera has been developed. Image analysis and densitometry are achieved with convenient control over image editing and calibration. - U Seppelt, E Sprenger, J Hedderich
Investigation of automated DNA diagnosis and grading of prostatic cancer
1986 Jun;8(2):152-7
Abstract: As a preliminary step towards a DNA-related automatic diagnosis and grading, the fine needle aspiration biopsies of 85 patients with prostatic cancer and of 20 patients with benign prostatic hypertrophy were subjected to DNA single-cell fluorescence cytometry. - V Cavallari, G Basile, A Maiorana, M C Maiorana
Personal computer software for the stereologic evaluation of sympathetic ganglia
1986 Mar;8(1):71-4
Abstract: Software was developed on a personal computer for a quantitative study of some ultrastructural aspects of sympathetic ganglia (the neuronal cell body, the surrounding satellite cell layer, and the nearby capillaries).
- R Jagoe, C Sowter
The delineation of nuclear boundaries in microscopic images
1986 Dec;8(4):305-13
Abstract: A semiautomatic procedure for tracking the boundaries of liver nuclei in digitized microscopic images of normally prepared biopsy material is described. - J P van Spreeuwel, J Lindeman, C J Meijer
Quantitative analysis of immunoglobulin-containing cells in gastrointestinal pathology
1986 Dec;8(4):314-20
Abstract: The morphometric quantitative analysis of immunoglobulin-containing cells in gastrointestinal biopsies was explored as a possible additional parameter in making the histologic diagnosis of gastrointestinal diseases. - J K Stevens, J Trogadis
Reconstructive three-dimensional electron microscopy. A routine biologic tool
1986 Jun;8(2):102-7
Abstract: Twenty years ago a laboratory could devote an entire year or more to the collection and analysis of a single set of serial electron micrographs. - E B Thunnissen, P C Diegenbach
Classification of lung carcinoma by means of digital nuclear image analysis
1986 Dec;8(4):301-4
Abstract: An investigation was performed of the maximum discriminating efficiency for each subgroup of digital nuclear image features and of the overall classification of nuclei from three types of human lung carcinomas in histologic sections: adenocarcinoma, small-cell carcinoma, and squamous-cell carcinoma. - W Auffermann, G R Krueger, A Böcking
DNA image cytometry in acquired immune deficiency syndrome (AIDS)
Comparative Study
Abstract: In nine cases with the acquired immune deficiency syndrome (AIDS), including four stage I cases, three stage II cases, and two stage III cases, DNA image cytometry was performed on Feulgen-stained lymph node imprint smears.