Volume 19 (2003) Issue 1 - Issue 6
- Ferran Algaba, Isabel Trias, Alfredo Santinelli, Rodolfo Montironi
TP53 in urologic tumors
Review: 2003 Jun;25(3):123-30
Abstract: TP53, a gene located on chromosome 17p13, encodes a nuclear protein (p53) involved in cell cycle regulation. This protein degrades in 20 minutes. However, the inactivated gene can produce a protein with a half-life 4-20 times longer than that of the wild type; it can be demonstrated by immunohistochemistry. - Lorenzo Cerroni, Gaia Goteri
Differential diagnosis between cutaneous lymphoma and pseudolymphoma
2003 Aug;25(4):191-8
Abstract: Differential diagnosis of cutaneous lymphoproliferative disorders represents one of the most vexing problems in dermatology and dermatopathology. For nearly a century the diagnosis has been based only upon clinicopathologic correlation. - Sudip K Sarker
Diagnostic use of mean nuclear area and nuclear DNA content in preoperative breast cancer cytology
Comparative Study: 2003 Apr;25(2):81-4
Abstract: Fine needle aspiration (FNA) cytology of breast lumps is a routine procedure, and the diagnostic accuracy can be 95%. Occasional discrepancies arise, and it would be valuable to have additional parameters for accurate diagnosis. - Ekin Ozgür Aktaş, Safiye Aktaş, Ragip Ortaç, Gülden Diniz
Role of vascularization in determining the time of hypoxic-ischemic encephalopathy in the neonate
2003 Jun;25(3):177-80
Abstract: To examine the role of vascularization in determining the time of hypoxic-ischemic encephalopathy (HIEP). - Christiane Pienna Soares, Carlos Benatti Neto, Paula Andrea Gabrielli Fregonezi, Debora Barreto Teresa, Raimunda Telma de Macedo Santos, Adhemar Longatto Filho, Marina Yoshiê Sakamoto Maeda
Computer-assisted analysis of p53 and PCNA expression in oral lesions infected with human papillomavirus
2003 Feb;25(1):19-24
Abstract: To carry out a retrospective study to determine whether human papillomavirus (HPV) infection and immunohistochemical expression of p53 and proliferating cell nuclear antigen (PCNA) are related to the risk of oral cancer. - Marco Wiltgen, Armin Gerger, Christian Wagner, Patrick Bergthaler, Josef Smolle
Discrimination of benign common nevi from malignant melanoma lesions by use of features based on spectral properties of the wavelet transform
2003 Oct;25(5):243-53
Abstract: To evaluate the possibilities of describing and discriminating common nevi and malignant melanoma tissue with features based on spectral properties of the Daubechies 4 wavelet transform. - Vivian Pierri Bersch, Vinicius Duval da Silva, Alessandro Bersch Osvaldt, Mário Sérgio Borges da Costa, Luiz Rohde, Diego Mossmann
Digital karyometry in pancreatic adenocarcinoma
2003 Apr;25(2):108-14
Abstract: To characterize nuclei from pancreatic adenocarcinoma and nonneoplastic pancreatic tissue by digital karyometry, demonstrating specific nuclear signatures for each of them. - Noel T Loughman, Betty P C Lin, Owen F Dent, Ronald C Newland
DNA ploidy of bladder cancer using bladder biopsy supernate specimens
2003 Jun;25(3):146-58
Abstract: To perform DNA image cytometry on 119 bladder biopsy supernate (BBS) specimens of transitional cell carcinoma (TCC) bladder to: (1) test the suitability of this cytologic specimen for use in DNA ploidy analysis, and (2) assess the value of DNA ploidy measured on this specimen as to the risk of tumor recurrence and survival. - Omer Günhan, Erhan Yildiz, Yildirim Karslioğlu, Yavuz Aydintuğ, Necdet Doğan, Bülent Celasun
Nuclear morphometric features of epithelial cells lining keratocysts
2003 Apr;25(2):85-9
Abstract: To investigate the nuclear morphometric features of epithelial cells lining keratocysts and some other odontogenic cysts. - Geula Klorin, Ruth Keren
Prognostic markers in histologic and cytologic specimens of breast cancer
2003 Oct;25(5):297-302
Abstract: To correlate histologic and cytologic specimens of breast cancer by the expression of prognostic factors, such as estrogen receptor (ER), progesterone receptor (PR) and c-erbB-2, with immunochemical staining. - Jens Krugmann, Andreas Gschwendtner, Thomas Mairinger, Falko Fend
DNA ploidy in gastrointestinal B-cell lymphomas. An image analysis study of 43 cases
2003 Feb;25(1):31-8
Abstract: To analyze the prognostic importance of DNA ploidy pattern on gastrointestinal (GI) B-cell lymphoma using image cytometry (ICM) and to compare the results with previously published flow cytometry (FCM) data. - Vito Rodolico, Anna Martorana, Gaspare Gulotta, Giuseppe Diana, Vincenza Leonardi, Gianfranco Cocorullo, Francesca Ajello
Prognostic significance of standardized AgNOR analysis and Ki-67 immunostaining in gastrointestinal stromal tumors
2003 Aug;25(4):199-209
Abstract: To evaluate the prognostic utility of argyrophilic nucleolar organizer region (AgNOR) protein parameters and Ki-67-immunostained growth fraction (Ki-67 labelling index) and to correlate AgNORs with Ki-67 LI and the main clinicopathologic parameters in gastrointestinal stromal tumors (GISTs). - Ziva Pohar-Marinsek, Matej Bracko, Jaka Lavrencak, Marija Us-Krasovec
DNA ploidy as a prognostic factor in rhabdomyosarcoma. Analysis of 35 cases with image cytometry
2003 Aug;25(4):235-42
Abstract: To correlate DNA ploidy in rhabdomyosarcoma (RMS) with other prognostic factors and patient survival and to search for possible reasons for inconsistent conclusions in similar, published studies. - Stefan Biesterfeld, Helen Louise Kraus, Thorsten Reineke, Leon Muys, Ana Maria Mihalcea, Christian Rudlowski
Analysis of the reliability of manual and automated immunohistochemical staining procedures. A pilot study
2003 Apr;25(2):90-6
Abstract: To study the variation in the number of stained cells and staining intensity comparing 2 immunostainers and manual staining for estrogen receptor (ER) expression in breast carcinoma. - Krzysztof Okon, Anna Szumera, Boleslaw Papla, Iwona Pietkun, Andrzej Zdunczyk, Malgorzata Rucinska, Aleksander B Skotnicki, Jerzy Stachura
Morphometric classification of hairy cell leukemia in bone marrow trephine biopsy
2003 Aug;25(4):227-34
Abstract: To analyze cytologic and histologic parameters in bone marrow trephine biopsy in an attempt to define heterogeneity of hairy cell leukemia cells. - Geula Klorin, Ruth Keren
Ploidy and nuclear area as a predictive factor of histologic grade in primary breast cancer
2003 Oct;25(5):277-80
Abstract: To compare ploidy and nuclear area with histologic grade in breast cancer using cytologic samples. - Reinhold Nafe, Wolfgang Schlote
Topometric analysis of diffuse astrocytomas
2003 Feb;25(1):12-8
Abstract: To investigate differences between astrocytomas of WHO grade 2 and anaplastic astrocytomas of WHO grade 3 in terms of topometric variables characterizing individual tumor cell nuclei. - Sermin Ozkal, Ozgül Sagol, Sedat Karademir, Ahmet Coker, Hüseyin Astarcioglu, Ali Küpelioglu, Ibrahim Astarcioglu, Omer Topalak, Yigit Göktay
Prognostic significance of mean nuclear volume in pancreatic adenocarcinoma
2003 Aug;25(4):221-6
Abstract: To evaluate the correlation of stereologically estimated mean nuclear volume of tumor cells with other clinicopathologic prognostic features and survival in pancreatic ductal adenocarcinoma. - Dragan Mihailovic, Desanka Tasic-Dimov, Zaklina Mijovic, Dragan Radovanovic
Nuclear volume and total optical density in Down syndrome
2003 Oct;25(5):293-6
Abstract: To estimate nuclear size and integrated optical density of parenchymal cells from various organs in patients with Down syndrome and a control group. - Margareta Strojan Flezar, Igor But, Rajko Kavalar, Marija Us-Krasovec
Flow and image cytometric DNA ploidy, including 5c exceeding cells, of serous borderline malignant ovarian tumors. Correlation with clinicopathologic characteristics
2003 Jun;25(3):139-45
Abstract: To analyze DNA ploidy of serous borderline ovarian tumors by flow cytometry (FCM) and image cytometry (ICM), with 5c exceeding cells also analyzed, and to evaluate their correlation with clinicopathologic characteristics of patients and tumors. - Neil Anderson, J Houghton, S J Kirk, Denise Frank, James Ranger-Moore, David S Alberts, Deborah Thompson, Peter H Bartels
Malignancy-associated changes in lactiferous duct epithelium
2003 Apr;25(2):63-72
Abstract: To determine whether cells from histologically normal appearing epithelium of the lactiferous duct from women with a remote ductal lesion in the breast provide any clues indicating the existence of such a lesion. - Neuza Kasumi Shirata, Adhemar Longatto Filho, Cecília Roteli-Martins, Luiz Marcelo W Espoladore, Janaína Erika Pittoli, Kari Syrjänen
Applicability of liquid-based cytology to the assessment of DNA content in cervical lesions using static cytometry
2003 Aug;25(4):210-4
Abstract: To evaluate the nuclear DNA content of cervical lesions in liquid-based cytologic specimens prepared for static cytometry. - Yasmin Anum Mohd Yusof, Khong Li Yan, Sharifah Noor Akmal Syed Hussain
Immunohistochemical expression of pi class glutathione S-transferase and alpha-fetoprotein in hepatocellular carcinoma and chronic liver disease
2003 Dec;25(6):332-8
Abstract: To determine whether tumor marker pi glutathione transferase (GST-pi) is expressed in hepatocellular carcinoma (HCC) and other chronic liver diseases and to compare its expression with that of alpha-fetoprotein (AFP). - Vladimir A Kirillov, Yury P Yuschenko, Andrey A Paplevka, Evgeny P Demidchik
Morphometric analysis of the degree of aggregation of follicular cells in the diagnosis of malignant thyroid tumors
2003 Jun;25(3):159-68
Abstract: To develop a system of decision criteria based on a set of features of the degree of thyrocyte aggregation with assessment of their weighting coefficient in the diagnosis of malignant and benign thyroid pathology. - Nicola Dioguardi, Barbara Franceschini, Giacomo Aletti, Carlo Russo, Fabio Grizzi
Fractal dimension rectified meter for quantification of liver fibrosis and other irregular microscopic objects
2003 Dec;25(6):312-20
Abstract: To introduce a new mathematical method based on the principles of fractal geometry analysis that permits more realistic quantification of some of the physical (morphologic) aspects of irregular bodies appearing under microscopy. - Adem Elzagheid, Yrjo Collan
Fine needle aspiration biopsy of the breast. Value of nuclear morphometry after different sampling methods
2003 Apr;25(2):73-80
Abstract: To study the potential of nuclear morphometry in supporting the interpretation of fine needle aspiration biopsy (FNAB) samples of the breast fixed in 50% ethanol and centrifuged on slides. - Ji Shin Lee, Hyung Seok Kim, Jong Tae Park, Min Cheol Lee, Chang Soo Park
Expression of vascular endothelial growth factor in the progression of cervical neoplasia and its relation to angiogenesis and p53 status
2003 Dec;25(6):303-11
Abstract: To evaluate vascular endothelial growth factor (VEGF) expression in the successive steps of cervical neoplasia and to determine its correlation with angiogenesis and p53 status. - Casey Diekman, Wei He, Nagabhushana Prabhu, Harvey Cramer
Hybrid methods for automated diagnosis of breast tumors
2003 Aug;25(4):183-90
Abstract: To design and analyze a new family of hybrid methods for the diagnosis of breast tumors using fine needle aspirates. - Elva I Cortés-Gutiérrez, Ricardo M Cerda-Flores, Diana S Leal-Klevezas, Fernando Hernández-Garza, Carlos H Leal-Garza
Validating polymerase chain reaction for detecting HPV in cervical intraepithelial neoplasia
2003 Apr;25(2):115-8
Abstract: To validate polymerase chain reaction (PCR) analysis for detecting HPV in Mexican women with cervical intraepithelial neoplasia, grade 2 and 3 (CIN 2/3) versus histologic evidence. - Sambit K Mohanty, Pranab Dey, Puja Rana
Manual and automated AgNOR count in differentiating reactive mesothelial from metastatic malignant cells in serous effusions
2003 Oct;25(5):273-6
Abstract: To distinguish reactive mesothelial cells from malignant cells in serous effusions using manual and automated methods of enumeration of argyrophilic nucleolar organizer regions (AgNORs). - Edmond Sabo, Myriam Gibrat, Yanina Sova, Avi Stein, Murray B Resnick
Validation of the novel indices of nuclear pleomorphism, polarity and spatial distribution in the grading of urothelial carcinoma
2003 Feb;25(1):53-62
Abstract: To evaluate the role of the novel histomorphometric variables of pleomorphism, orientation and variability of spatial cytologic organization to objectively quantify histopathologic grade in urothelial carcinoma. - Reinhold Nafe, Sebastian Herminghaus, Peter Raab, Sabine Wagner, Ulrich Pilatus, Wolfgang Schlote, Friedhelm Zanella, Heinrich Lanfermann
Correlation between preoperative magnetic resonance spectroscopic data on high grade gliomas and morphology of Ki-67-positive tumor cell nuclei
2003 Jun;25(3):131-8
Abstract: To investigate possible statistical correlations between metabolic data from preoperative proton magnetic resonance spectroscopy (1HMRS) and morphology of proliferating tumor cell nuclei in anaplastic gliomas and glioblastomas. - Francisco A Garcia, John R Davis, David S Alberts, Yun Liu, Deborah Thompson, Peter H Bartels
A karyometric approach to the characterization of atypical endometrial hyperplasia with and without co-occurring adenocarcinoma
2003 Dec;25(6):339-46
Abstract: To develop a karyometric image analysis approach to distinguishing atypical endometrial hyperplasia with and without co-occurring adenocarcinoma. - Aydin Işisağ, Nalan Neşe, Murat Ermete, Murat Lekili, Semin Ayhan, Ali Riza Kandiloğlu
Col IV and Fn distribution in prostatic adenocarcinoma and correlation of 67LR, MMP-9 and TIMP-1 expression with Gleason score
2003 Oct;25(5):263-72
Abstract: To assess the immunoreactivity of 5 proteins related to basement membrane (BM) and extracellular matrix in order to investigate whether any of them correlates with differentiation of prostatic adenocarcinoma (PAc). Two of these markers are collagen type IV (Col IV), the collagenous component of basement membrane, and fibronectin (Fn), an adhesion protein in extracellular matrix. Others are matrix metalloproteinase-9 (MMP-9), a type IV collagenase, tissue inhibitor of matrix metalloproteinase-1 (TIMP-1), which has a high affinity for MMP-9, and 67-kd laminin receptor (67LR), which belongs to the non-integrin laminin binding receptor family. - Dorota Słowińska-Klencka, Mariusz Klencki, Stanisław Sporny, Bozena Popowicz, Andrzej Lewiński
Cytologic appearance of toxic nodular goiter after thyrostatic treatment. A karyometric study
2003 Feb;25(1):39-46
Abstract: To assess whether the cytologic appearance of aspirates from toxic nodular goiter is substantially modified during the course of therapy with thyrostatic drugs. - Ekaterini N Politi, Andreas C Lazaris, Nikolaos Kavantzas, Helen Koutselini
Comparison between morphometry and immunostaining of malignant cells in non-small cell lung cancer
2003 Jun;25(3):169-76
Abstract: To investigate the morphometric properties of tumor cells in combination with the expression of 5 immunomarkers, quantitatively evaluated by image analysis, in a series of 60 non-small cell lung carcinomas (NSCLCs) and to assess their prognostic significance. - Paul Bozzo, Kathylynn Saboda, Janine G Einspahr, James Ranger-Moore, Evan R Farmer, Clay J Cockerell, David E Elder, Jerry L Bangert, Nancy Hart, Cheryl B Kramer, David S Alberts
Reliability and validity of a histologic score as a marker for skin cancer chemoprevention studies
2003 Oct;25(5):285-92
Abstract: To develop a reliable and valid scoring system for grading skin biopsies from actinic keratosis (AK) and sun-damaged skin for use in evaluating the efficacy of skin cancer chemopreventive agents. - James Ranger-Moore, Paul Bozzo, David Alberts, Janine Einspahr, Yun Liu, Deborah Thompson, Steven Stratton, M Suzanne Stratton, Peter Bartels
Karyometry of nuclei from actinic keratosis and squamous cell cancer of the skin
2003 Dec;25(6):353-61
Abstract: To use karyometric analysis methods to compare actinic keratoses (AKs) to squamous cell carcinomas (SCCs) to determine if SCCs showed a logical progression beyond that seen in AKs and to explore variability within and between lesion types to better understand distinctions between the 2. - Larry Latson, Bruce Sebek, Kimerly A Powell
Automated cell nuclear segmentation in color images of hematoxylin and eosin-stained breast biopsy
2003 Dec;25(6):321-31
Abstract: To develop an automated, reproducible epithelial cell nuclear segmentation method to quantify cytologic features quickly and accurately from breast biopsy. - Marluce Bibbo, Jennifer DeCecco, Albert J Kovatich
P16INK4A as an adjunct test in liquid-based cytology
2003 Feb;25(1):8-11
Abstract: To assess the utility of P16INK4A as an adjunct test in liquid-based cytology in cases with equivocal morphologic changes of high grade squamous intraepithelial lesion (HSIL). - Dorota Słowińska-Klencka, Mariusz Klencki, Bozena Popowicz, Andrzej Lewiński
AgNOR quantification in the diagnosis of follicular pattern thyroid lesions
2003 Dec;25(6):347-52
Abstract: To evaluate the usefulness of silver staining of nucleolar organizer regions (AgNORs) in the preoperative diagnosis of follicular lesions in the thyroid with computer-aided morphometric analysis of the silver dots. - Marcelo Emílio Beletti, Luciano da Fontoura Costa
A systematic approach to multispecies sperm morphometric characterization
Comparative Study: 2003 Apr;25(2):97-107
Abstract: To investigate the morphologic variety of sperm heads between several species through a comprehensive set of measurements, some of which are presented here for the first time, and to perform cluster analysis. - Beatriz L Molinari, Débora R Tasat, Mónica A Palmieri, Silvia E O'Connor, Rómulo L Cabrini
Cell-based quantitative evaluation of the MTT assay
2003 Oct;25(5):254-62
Abstract: To analyze the bioreduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) on a per cell basis and evaluate its modulation as a function of different stages of cell metabolism. - Logasundaram Rajesh, Pranab Dey
Fractal dimensions in urine smears: a comparison between benign and malignant cells
Comparative Study: 2003 Jun;25(3):181-2
Abstract: To compare fractal dimensions in urine smears between benign and malignant cells. - Kitty F Linssen, Jan A Jacobs, Fred H Nieman, Lisette E Cornelissen, Marjolein Drent
Use of poly-l-lysine-coated slides in clinical bronchoalveolar lavage fluid samples
2003 Oct;25(5):281-4
Abstract: Polylysine coating of microscope slides provides superior cell adhesion. We compared poly-l-lysine-coated (PLC) slides to conventional slides in cytocentrifuged bronchoalveolar lavage (BAL) fluid samples. - Mario A Rosito, Luis F Moreira, Vinicius D da Silva, Daniel C Damin, João C Prolla
Nuclear chromatin texture in rectal cancer. Relationship to tumor stage
2003 Feb;25(1):25-30
Abstract: To characterize, by morphometric and chromatin texture analysis, a series of rectal carcinomas classified according to Dukes staging. - Mario A Rosito, Daniel C Damin, Luis F Moreira, Vinicius D da Silva, João C Prolla
Nuclear chromatin texture in rectal carcinoma. Prognostic value
2003 Aug;25(4):215-20
Abstract: To investigate the prognostic value of nuclear features in rectal carcinoma. - Pauliina Kronqvist, Teijo Kuopio, Yrjö Collan
Effect of freezing on histologic grading of invasive ductal breast cancer
2003 Feb;25(1):47-52
Abstract: To quantify the histologic changes caused by freezing during tissue processing and their influence on histologic malignancy grading as a prognostic factor in invasive ductal breast cancer. - Cytopathology Education and Technology Consortium (CETC)
Daily workload guidelines for cytotechnologists utilizing automated, assisted-screening technologies
2003 Apr;25(2):119-22
Abstract: To develop guidelines for daily workload for cytotechnologists utilizing automated, assisted-screening technologies. - Galliano Tinacci, Maria Paola Cariaggi, Francesca Carozzi, Alessandro Foggi, Guido Miccinesi, Francesco Mirri, Paolo Pasquini, Marco Zappa, Massimo Confortini
Digital images for interobserver variability comparison in cervicovaginal cytology
Comparative Study: 2003 Feb;25(1):1-7
Abstract: To assess whether digital images can be useful in evaluating interobserver variability in cervical-vaginal cytology.