Volume 17 (2001) Issue 1 - Issue 6
- A C Ruifrok, D A Johnston
Quantification of histochemical staining by color deconvolution
Comparative Study: 2001 Aug;23(4):291-9
Abstract: To develop a flexible method of separation and quantification of immunohistochemical staining by means of color image analysis. - V D da Silva, J C Prolla, P Sharma, R Sampliner, D Thompson, P H Bartels
Karyometry in Barrett's esophagus
2001 Feb;23(1):40-6
Abstract: To derive a progression curve for lesions in Barrett's esophagus based on karyometric features. - M C Chalita, J M Matera, M T Alves, A Longatto Filho
Nonaspiration fine needle cytology and its histologic correlation in canine skin and soft tissue tumors
Comparative Study: 2001 Dec;23(6):395-9
Abstract: To analyze the findings of nonaspiration fine needle (NAFN) cytology as compared with the histopathologic findings in evaluating canine skin and soft tissue tumors. - P H Bartels, F A Garcia, J Davis, V D da Silva, H G Bartels, D Thompson, D S Alberts
Progression curves for endometrial lesions
2001 Feb;23(1):1-8
Abstract: To derive a numeric measure for the progression of endometrial lesions as a baseline study for an eventual assessment of chemopreventive intervention efficacy. - M H Weinstein, P Dal Cin
Genetics of epithelial tumors of the renal parenchyma in adults and renal cell carcinoma in children
Review: 2001 Oct;23(5):362-72
Abstract: The classification of renal cell carcinomas has been affected by both the delineation of new histologic subtypes and the understanding that recognized histomorphologic patterns are reflective of specific sets of cytogenetic abnormalities. - E Ruijter, R Montironi, C van de Kaa, J Schalken
Molecular changes associated with prostate cancer development
Review: 2001 Feb;23(1):67-88
Abstract: The epidemiologic characteristics of prostate cancer (PCa) have been recognized for several decades. - P Bozzo, D S Alberts, L Vaught, V D da Silva, D Thompson, J Warnecke, R C Miller, J Einspahr, P H Bartels
Measurement of chemopreventive efficacy in skin biopsies
Comparative Study: 2001 Aug;23(4):300-12
Abstract: To explore methods suitable for quantitative assessment of the efficacy of chemopreventive intervention. - D Thompson, D Richards, H Bartels, R Montironi, M Scarpelli, P W Hamilton, P H Bartels
Multimegapixel images in histopathology
2001 Jun;23(3):169-77
Abstract: To describe methods and procedures for the assembly of very large scale microscopic image arrays. - N Pomjanski, H Motherby, B Buckstegge, K Knops, B L Rohn, A Böcking
Early diagnosis of mesothelioma in serous effusions using AgNOR analysis
Comparative Study: 2001 Apr;23(2):151-60
Abstract: To compare the results of conventional cytology, DNA image cytometry, immunocytochemistry and argyrophilic nucleolar organizer region (AgNOR) analysis for the diagnosis of malignant cells in serous effusions. - D Cruz, C Valentí, A Dias, M Seixas, F Schmitt
Microvessel density counting in breast cancer. Slides vs. digital images
2001 Feb;23(1):15-20
Abstract: To develop a program to assist the pathologist in the acquisition and evaluation of digital images to determine microvessel density (MVD) in tissues. - C Maohuai, A R Chang, D Lo
Nasopharyngeal carcinoma heterogeneity of DNA content identified on cytologic preparations
2001 Jun;23(3):213-7
Abstract: To evaluate tumor heterogeneity of DNA content in nasopharyngeal carcinoma (NPC) performed on cytologic specimens. - C Giardina, D M Caniglia, T Lettini, T Valente, G Poliseno, L Tantimonaco, G Favia
Morphometric discrimination between syndromic (Gorlin) and nonsyndromic keratocysts
Comparative Study: 2001 Oct;23(5):373-80
Abstract: To search for morphologic nuclear features in the epithelial lining of odontogenic keratocysts to differentiate simple from Gorlin syndrome cysts. - U Brahmi, A Rajwanshi, K Joshi, P Dey, H Vohra, N K Ganguly, S K Gupta
Flow cytometric immunophenotyping and comparison with immunocytochemistry in small round cell tumors
Comparative Study: 2001 Dec;23(6):405-12
Abstract: To quantitate different antigens by flow cytometric immunophenotyping (FCI) in small round cell tumors (SRCTs) and to compare the FCI technique with immunocytochemistry (IC). - M dos Anjos Pires, C Palmeira, P Rodrigues, C Lopes, F Oliveira-Torres
Establishment of a diploid reference value for DNA ploidy analysis by image cytometry in mouse cells
2001 Dec;23(6):427-32
Abstract: To establish a diploid reference value for DNA ploidy analysis of mouse cells (Mus musculus) by image cytometry using the CAS 200, an analysis system suitable for DNA content studies in human cells. - D Cruz, N Melo, M Seixas, J M Lopes
Measuring beyond the microscope field of view using digital images in squamous cell carcinoma of the lip
Comparative Study: 2001 Aug;23(4):245-50
Abstract: To study the impact of using digital images for measuring the size of the tumors, assisting with the prognostic evaluation of carcinomas of the oral mucosa. - F A Garcia, J R Davis, D S Alberts, K Hatch, B Weyn, D Thompson, P H Bartels
Nuclear chromatin patterns in normal, hyperplastic and atypical endometrium
Comparative Study: 2001 Apr;23(2):144-50
Abstract: To characterize the nuclei of endometrial lesions for the diagnostic categories of normal glandular tissue, simple hyperplasia, atypical hyperplasia and adenocarcinoma of the endometrium, with the specific goal of probing for heterogeneity. - S Biesterfeld, M Leitloff, W Rath, W Schröder
DNA image cytometry in the differential diagnosis of endometrial hyperplasia and adenocarcinoma
Comparative Study: 2001 Apr;23(2):123-8
Abstract: To test the value of DNA image cytometry in the differential diagnosis of hyperplastic endometrial lesions and endometrial carcinoma on a series of 153 cases of simple hyperplasia (n = 71), complex hyperplasia (n = 28), complex atypical hyperplasia (n = 11) and endometrial adenocarcinoma (n = 43). - J Azúa-Romeo, J de Azúa-Blanco, P Romeo
Image cytometry in nonproliferative fibrocystic breast changes. Ploidy evaluation and epidemiologic study
Comparative Study: 2001 Apr;23(2):129-34
Abstract: To determine the usefulness of image cytometry on fine needle aspirates from patients with fibrocystic changes (FCCs) to assess the subsequent risk of breast cancer. - E J Weaver, P A McCue, D H Bagley, A J Kovatich, M Bibbo
Expression of cytokeratin 20 and CD44 protein in upper urinary tract transitional cell carcinoma: cytologic-histologic correlation
Comparative Study: 2001 Oct;23(5):339-44
Abstract: To explore the potential utility of immunostaining for CK20 and CD44 protein isoforms in evaluating cases of upper urinary tract transitional cell carcinoma (UTTCC). - M Skacel, J D Pettay, E K Tsiftsakis, G W Procop, C V Biscotti, R R Tubbs
Validation of a multicolor interphase fluorescence in situ hybridization assay for detection of transitional cell carcinoma on fresh and archival thin-layer, liquid-based cytology slides
2001 Dec;23(6):381-7
Abstract: To evaluate the feasibility of performing multicolor interphase fluorescence in situ hybridization (FISH) on ThinPrep slides of transitional cell carcinoma (TCC). - L M Pereira, C A Mandarim-de-Lacerda
Stereology of cardiac hypertrophy induced by NO blockade in rats treated with enalapril and verapamil
2001 Oct;23(5):330-8
Abstract: To quantify the structural myocardial response when chronic NO blockade hypertension is treated with antihypertensive drugs. - C Maohuai, A R Chang, L Shikyuen
Roles of DNA cytometry and detection of EBERs in predicting a diagnosis of nasopharyngeal carcinoma
Comparative Study: 2001 Jun;23(3):207-12
Abstract: To analyze the suitability of DNA cytometry and detection of Epstein-Barr virus (EBV)-encoded RNAs (EBERs) on nasopharyngeal brushings for predicting a diagnosis of nasopharyngeal carcinoma (NPC). - M R Cardillo, D Lazzereschi, O Gandini, F Di Silverio, G Colletta
Transforming growth factor-beta pathway in human renal cell carcinoma and surrounding normal-appearing renal parenchyma
Comparative Study: 2001 Apr;23(2):109-17
Abstract: To analyze the role of the transforming growth factor (TGF)-beta pathway in renal tumors and to verify whether alterations in TGF-beta 1 pathway expression are associated with the grade of tumor differentiation and pathologic stage in renal cell carcinomas. - J Laitakari, D Harrison, F Stenbäck
Morphometric characteristics of cell proliferation and p53 expression in development of experimentally induced respiratory tumors
2001 Aug;23(4):273-86
Abstract: To study, under controlled conditions, the applicability of automated image analysis of immunohistochemical markers as an indicator of development and progression in tobacco component-induced tumors in the respiratory tract. - M Bracko, M Us-Krasovec, T Cufer, J Lamovec, A Zidar, W Goehde
Prognostic significance of DNA ploidy determined by high-resolution flow cytometry in breast carcinoma
2001 Feb;23(1):56-66
Abstract: To assess the prognostic value of DNA ploidy in breast carcinoma and its relation to other established prognostic factors. - H Gundlach
Digital microscopy for multiparameter FISH imaging
2001 Aug;23(4):268-72
Abstract: Fluorescence in situ hybridization (FISH) allows the direct localization of DANN and RNA sequences on chromosomes, in cells and in tissue. The technique is based on hybridization between target sequences of single-strand DNA of chromosomes or cell nuclei with marked complementary specimens. - D G Menter, A Hoque, N Motiwala, A A Sahin, N Sneige, R Lieberman, S M Lippman
Computerized image analysis of Ki-67 in ductal breast carcinoma in situ
2001 Jun;23(3):218-28
Abstract: To develop and determine the staining protocols and computerized image analysis methods that are the most effective combination for performing quantitative analysis of Ki-67. - P Dey, U K Luthra, S S George, B I Haji, J George
Terminal uridine nick end labelling and mitosis in breast carcinoma. Correlation with tumor grade and p53 overexpression
2001 Feb;23(1):27-30
Abstract: To study the relationship of cell death and proliferation to histologic grade and p53 expression in invasive carcinoma of the breast. - R Nafe, W Glienke, W Schlote, B Schneider
EGFR gene amplification in glioblastomas. Is there a relationship with morphology of tumor cell nuclei and proliferative activity?
Comparative Study: 2001 Apr;23(2):135-43
Abstract: To confirm a relationship between histomorphology of glioblastomas and amplification of the gene for the epidermal growth factor receptor (EGFR) as the most important molecular biologic alteration in these tumors. - H van Goor, W Coers, M L van der Horst, S Huitema, A J Suurmeijer
Distribution of cytoskeletal proteins, integrins, leukocyte adhesion molecules and extracellular matrix proteins in plastic-embedded human and rat kidneys
2001 Oct;23(5):345-54
Abstract: To study the distribution of cytoskeletal proteins (actin, alpha-actinin, vinculin, beta-tubulin, keratin, vimentin, desmin), adhesion molecules for cell-matrix interations (very later antigens [VLA1-61, beta1, beta2 [CD18], vitronectin receptor [alphavbeta3], CD 11b), leukocyte adhesion molecules (ICAM-1) and extracellular matrix proteins (collagen IV, fibronectin, laminin, vitronectin) in human and rat kidneys by using a superior processing and immunohistochemical staining technique. - V Lousuebsakul, S M Knutsen, P N Singh, I T Gram
Variables associated with squamous intraepithelial lesions among women with a cytologic diagnosis of atypical squamous cells of undetermined significance
2001 Oct;23(5):355-61
Abstract: To identify factors among women with atypical squamous cells of undetermined significance (ASCUS) cytologic diagnosis that are associated with either low grade squamous intraepithelial lesions (LSILs) or high grade squamous intraepithelial lesions (HSILs). - G Giuffrè, E Fulcheri, M Gualco, F Fedele, G Tuccari
Standardized AgNOR analysis as a prognostic parameter in endometrial carcinoma, endometrioid type
2001 Feb;23(1):31-9
Abstract: To evaluate interobserver reproducibility of histologic grade in endometrial adenocarcinomas of endometrioid type (EC), to assess the relationships between nuclear grade and the amount of argyrophilic nucleolar organizer region (AgNOR) proteins and to determine the prognostic value of AgNOR proteins and the main clinicopathologic parameters. - T Mirtti, M Kallajoki, M Aaltonen, K Alanen
Cyclin A and Ki-67 with DNA content in benign and malignant prostatic epithelial lesions
2001 Jun;23(3):229-37
Abstract: To evaluate the usefulness of immunohistochemical staining of cyclin A and Ki-67 together with DNA content in the classification of benign prostatic hyperplasia, prostatic intraepithelial neoplasm (PIN) and prostatic carcinoma foci and to compare these parameters with each other and with parameters obtained from conventional histopathology. - O Sağol, N Culhaci, K Yörükoğlu, E Ozen, T Canda
Quantification of tumor cellularity and mitotic index in invasive ductal carcinoma of the breast
2001 Feb;23(1):21-6
Abstract: To evaluate the use of stereologically estimated tumor cell counts in the mitotic index as well as to investigate its correlation with the currently used method and test the reproducibility of the method. - U Brahmi, A Rajwanshi, K Joshi, P Dey, H Vohra, N K Ganguly, S K Gupta
Automated nuclear image morphometry on fine needle aspiration smears of malignant round cell tumors
Comparative Study: 2001 Aug;23(4):287-90
Abstract: To analyze nuclear image morphometry in fine needle aspiration cytology smears of different groups of malignant round cell tumors (MRCTs) to evaluate its diagnostic role. - P Zeppa, G Benincasa, G Troncone, A Zabatta, F Fulciniti, A Vetrani, E A Palmieri, L Palombini
Quantitative assessment of oxyphilic cell lesions of the thyroid gland on fine needle aspiration samples
2001 Jun;23(3):178-84
Abstract: To assess the possible contribution by a multiparametric quantitative approach to the cytologic diagnosis of oxyphilic cell (OC) thyroid lesions. - J S Lee, H S Kim, J J Jung, Y B Kim, C S Park, M C Lee
Correlation between angiogenesis, apoptosis and cell proliferation in invasive ductal carcinoma of the breast and their relation to tumor behavior
2001 Apr;23(2):161-8
Abstract: To investigate the relationship between angiogenesis, apoptosis and cell proliferation in invasive ductal carcinoma of the breast and their relation to tumor behavior. - S Schüpp, A Elmoataz, P Herlin, D Bloyet
Mathematical morphologic segmentation dedicated to quantitative immunohistochemistry
Comparative Study: 2001 Aug;23(4):257-67
Abstract: To develop automatic segmentation sequences for fully automated quantitative immunohistochemistry of cancer cell nuclei by image analysis. - M Berezuk, J West, M Varella-Garcia, W A Franklin
Adjusting interphase FISH results in epithelial tissue sections to whole cell complement
2001 Apr;23(2):93-100
Abstract: To derive an equation to compensate for the discrepancies between whole cell preparations and tissue sections for more accurate enumeration of fluorescence in situ hybridization (FISH) signals per cell. - K Y Jang, M J Kang, D G Lee, M J Chung
Utility of thyroid transcription factor-1 and cytokeratin 7 and 20 immunostaining in the identification of origin in malignant effusions
2001 Dec;23(6):400-4
Abstract: To estimate the utility of thyroid transcription factor-1 (TTF-1) and the combined cytokeratin 7 (CK7) and 20 (CK20) immunoprofile as a marker for identifying the primary site of metastatic adenocarcinoma in effusions of the serous cavity. - T Dreyer, I Knoblauch, A Doudkine, C E MacAulay, D Garner, B Palcic, C Popella
Nuclear texture features for classifying benign vs. dysplastic or malignant squamous epithelium of the larynx
2001 Jun;23(3):193-200
Abstract: To search for nuclear features and feature combinations able to assess malignancy and premalignant changes on tissue sections of laryngeal squamous epithelium. - N Mollaoglu, J G Cowpe, R Walker
Quantitative cytomorphologic analyses of Papanicolaou-stained smears from oral lichen planus
Comparative Study: 2001 Apr;23(2):118-22
Abstract: To apply quantitative techniques to cytologic smears in order to improve the diagnostic sensitivity of cytology in the detection of malignant change in the oral cavity. - E I Cortés-Gutiérrez, E Leal-Elizondo, R M Cerda-Flores, C H Leal-Garza
Polymorphism of Ag (+)-NORs in cervical smears from women with cervical cancer
2001 Feb;23(1):9-14
Abstract: To evaluate Ag(+)-stained (Ag(+)-NOR) polymorphism in four groups of patients with various grades of cervical lesions and in a control group. - M B Kenny-Moynihan, P B DeRose, A A Gal, H Chakraborty, C Cohen
Flow and image cytometry in thymic neoplasia: correlation with clinical outcome
Comparative Study: 2001 Aug;23(4):313-20
Abstract: To compare nuclear DNA by flow (FCM) and image cytometry (ICM) in thymic neoplasms and to relate results to clinical outcome. - A Buhmeida, H Kujari, Y Collan
Nucleolar morphometry in fine needle aspiration biopsies of the prostate
2001 Jun;23(3):185-92
Abstract: To find efficient morphometric nucleolar features for distinguishing different prostatic atypia groups in fine needle aspiration biopsies. - D H Frank, J R Davis, D S Alberts, D Thompson, Y Liu, P H Bartels
Nuclear chromatin characteristics of breast solid pattern ductal carcinoma in situ
2001 Dec;23(6):418-26
Abstract: To characterize nuclei from breast solid pattern ductal carcinoma in situ (DCIS) by their karyometric features and to search for the presence of statistically significantly different subsets of nuclei. - E Ozer, K Yörükoğlu, M U Mungan, S Ozkal, D Demirel, O Sağol, Z Kirkali
Prognostic significance of nuclear morphometry in superficial bladder cancer
Comparative Study: 2001 Aug;23(4):245-50
Abstract: To evaluate the significance of nuclear morphometry in predicting the clinical course in superficial (pTa and pT1) bladder cancer. - S Biesterfeld, M Kebernik
Limited value of PCNA immunohistometry on tru-cut biopsies in inflammatory and cirrhotic liver disease
Comparative Study: 2001 Dec;23(6):433-4 - A K Khanna, M A Ansari, M Kumar, A Khanna
Correlation between AgNOR count and subjective AgNOR pattern assessment score in cytology and histology of breast lumps
Comparative Study: 2001 Dec;23(6):388-94
Abstract: To find the correlation of argyrophilic nucleolar organizer region (AgNOR) count and subjective AgNOR pattern assessment (SAPA) score in cytology and histology of breast lumps. - E Kahn, G Lizard, F Frouin, C Souchier, J C Bernengo, H Siitari, C A Cuenod, O Clément, G Frija, P Gambert, A Todd-Pokropek
Confocal image characterization of human papillomavirus DNA sequences revealed with Eu in HeLa cell nuclei stained with Hoechst 33342
2001 Apr;23(2):101-8
Abstract: To visualize and localize specific viral DNA sequences revealed with Eu by fluorescence in situ hybridization, confocal laser scanning microscopy (CLSM) and factor analysis of biomedical image sequences (FAMIS). - K Yörükoğlu, S Sayhan, N Dicle
Tumor volume estimation by the percentage carcinoma method in uterine cervix carcinoma
2001 Feb;23(1):89-92
Abstract: To assess the capacity of the percentage carcinoma method to predict tumor volume in cervical carcinoma and generate a mathematical equation for calculation of tumor volume. - S K Sarker, K S Patel
Mean nuclear area of squamous cell carcinomas of the head and neck using image cytometry
Comparative Study: 2001 Dec;23(6):413-7
Abstract: To determine if mean nuclear area (MNA) in squamous cell carcinomas of the head and neck (SCCHN) correlate with the TNM system and histologic grade. - A Dulewicz, B D Pietka, P Jaszczak, A Nechay, W Sawicki, R Pykalo, E Kozminska, A Borkowski
Computer identification of neoplastic urothelial nuclei from the bladder
2001 Oct;23(5):321-9
Abstract: To introduce computer-based analysis of Feulgen-stained urinary bladder cell nuclei from voided urine to identify neoplastic urothelial nuclei. - E Kahn, G Lizard, F Frouin, J C Bernengo, C Souchier, G Bessède, O Clément, H Siitari, P Gambert, G Frija, A Todd-Pokropek
Confocal analysis of phosphatidylserine externalization with the use of biotinylated annexin V revealed with streptavidin-FITC, -europium, -phycoerythrin or -Texas Red in oxysterol-treated apoptotic cells
2001 Feb;23(1):47-55
Abstract: To analyze externalization of phosphatidylserine via annexin V on apoptotic cells by laser scanning confocal microscopy and factor analysis of biomedical image sequences (FAMIS). - O Nunobiki, M Sato, E Taniguchi, Y Nakamura, I Mori, K Kakudo
Morphometric characterization of endometrial glands using quantitative cytology
2001 Jun;23(3):201-6
Abstract: To determine the usefulness of the combination of confocal laser scanning microscopy (CLSM), image cytometry and three-dimensional (3D) imaging for analyzing architectural changes indicative of endometrial hyperplasia and grade 1 adenocarcinoma. - A García-Escudero, M de Miguel-Rodríguez, A Moreno-Fernández, G Navarro-Bustos, H Galera-Ruiz, H Galera-Davidson
Prognostic value of DNA flow cytometry in sympathoadrenal paragangliomas
2001 Jun;23(3):238-44
Abstract: To determine whether ploidy patterns are related to prognosis in sympathoadrenal paragangliomas (SAP) using flow cytometry.