Volume 12 (1996) Issue 1 - Issue 6
- C Giardina, G Renzulli, G Serio, D M Caniglia, T Lettini, C Ferri, G D'Eredità, R Ricco, V P Delfino
Nuclear morphometry in node-negative breast carcinoma
1996 Oct;18(5):374-82
Abstract: To determine whether nuclear morphometry can confirm or add useful information to classic clinicopathological prognosticators to identify the subpopulation of breast carcinoma patients with node-negative (N-) disease, at high risk of disease relapse. - B O Stokes, E Blee, M L Grover
Influence of fluid absorption time on cell recovery in cytocentrifugation
1996 Oct;18(5):389-99
Abstract: To determine the absorption time required to give complete cell recovery in cytocentrifugation. - P Fattoretti, C Bertoni-Freddari, U Caselli, R Paoloni, W Meier-Ruge
Morphologic changes in cerebellar mitochondria during aging
1996 Jun;18(3):205-8
Abstract: To seek age-dependent morphofunctional changes in mitochondrial metabolic competence in the rat cerebellum. - P Dalla Palma, M F Mauri, S Forti, C Eccher, R Antolini
Grading in superficial papillary bladder carcinoma, with an emphasis on nuclear orientation
1996 Aug;18(4):305-8
Abstract: To develop a computerized analysis method that could reflect not only cellular anaplasia but also the orientation (PV) of neoplastic elements and could allow the separation of low (grade G 1 and G 2a) and high (G 2b) grades of superficial papillary bladder tumors (SPBTs). - P Karakitsos, E B Stergiou, A Pouliakis, M Tzivras, A Archimandritis, A I Liossi, K Kyrkou
Potential of the back propagation neural network in the discrimination of benign from malignant gastric cells
1996 Jun;18(3):245-50
Abstract: To investigate the potential value of morphometry and the back propagation neural network for the discrimination of benign and malignant gastric lesions. - W F Whimster, P W Hamilton, N A Anderson, S Humphreys, M Boyle, M Sundaresan, A Rainey, A Giles, D Hopster, P H Bartels
Reproducibility of Bayesian belief network assessment of breast fine needle aspirates
Comparative Study: 1996 Oct;18(5):410-9
Abstract: To assess the consistency of diagnosis of fine needle aspiration biopsies of breast lesions by three experienced and five less experienced pathologists using conventional means and applying a Bayesian belief network (BBN) to 10 diagnostic features to support diagnostic decision making. - D Serón, F Moreso, C Gratin, J Vitriá, E Condom, J M Grinyó, J Alsina
Automated classification of renal interstitium and tubules by local texture analysis and a neural network
1996 Oct;18(5):410-9
Abstract: To segment renal interstitial space in order to automatically quantify renal cortical interstitial volume fraction (Vvint/cortex) by means of image analysis techniques. - Z Zhang, M Nakamura, E Taniguchi, L Shan, T Yokoi, K Kakudo
Late occurrence of K-ras gene mutations in the pathogenesis of squamous cell carcinoma of the lung: analysis in sputum
1996 Dec;18(6):501-2 - G Landini, J W Rippin
Quantification of nuclear pleomorphism using an asymptotic fractal model
1996 Apr;18(2):167-76
Abstract: Nuclear pleomorphism (nuclear membrane irregularity) was investigated using transmission electron microscope (TEM) micrographs of 1,419 nuclei (32 oral carcinomas and normal cells from surgical margins). - M Ladekarl, V Jensen, B Nielsen
Stereologic estimation of breast tumor size
1996 Apr;18(2):151-7
Abstract: The largest tumor diameter, D(T), is a variable of great clinical value in breast cancer but a biased and imprecise estimator of real tumor size. Three-dimensional, shape-independent estimates would more realistically reflect the tumor bulk and provide more accurate clinical staging. For experimental oncology, the measurements may be useful for precise assessment of tumor burden. - C Caldani, D F Far, I Birtwisle-Peyrottes, F Ettore, P Rostagno
Cell cycle expression of p53 protein, c-Myc gene product and tyrosine-phosphorylation level determined by image analysis in human breast cancer cells
1996 Jun;18(3):233-40
Abstract: To investigate the cell cycle expression of p53 protein, c-myc gene product and tyrosine phosphorylation level in human breast cancer cells. - S N O'Brien, K A Mantzke, M W Kilgore, T M Price
Relationship between adipose stromal-vascular cells and adipocytes in human adipose tissue
1996 Apr;18(2):137-43
Abstract: To determine the relative amounts of RNA and DNA from adipocytes and adipose stromal-vascular cells to better assess differential gene expression for estrogen receptor and cytochrome P450 aromatase in the two cell fractions of adipose tissue. - J Smolle, R Hofmann-Wellenhof, R Fink-Puches, N Auersperg
Assessment of tumor cell cohesion in vivo using pattern interpretation by cellular automata
1996 Jun;18(3):199-204
Abstract: There is abundant evidence that patterning in life sciences may be closely related to the functional properties of the constitutive elements. In order to estimate some of these functional properties by examining the static pattern evolved, pattern interpretation by cellular automata (PICA) was introduced and applied to the mechanisms of tumor growth. Previously it was shown that the estimates concerning tumor cell proliferation and motility were consistent with those obtained by other methods. - C Minimo, P A McCue, A Pindzola, J Brennan, M Bibbo
Role of computed quantitation of immunohistochemical staining of Ki-67 antigen in diagnosing ampullary lesions
1996 Oct;18(5):400-4
Abstract: To determine the possible role of Ki-67 antigen expression by visual and computed quantitation in diagnosing ampullary lesions. - C A Scott, L Desinan, M Maffezzini, A Simonato, C Avellini, S De Stefani, V Rizzi, G Carmignani, C A Beltrami
Effects of cis-platinum and luteinizing hormone releasing hormone analogues on rat spermatogenesis. A morphologic and flow cytometric study
1996 Oct;18(5):361-73
Abstract: To evaluate morphologically and flow cytometrically the effects of cis-platinum (cis-diaminedichloroplatinum [CDDP]) administered acutely and chronically with and without gonadotropin releasing hormone analogue (LRA) pretreatment on adult rats to verify the feasibility of protecting the spermatogenic epithelium before chemotherapy. - S Roels, R Ducatelle, H Cornelissen
Quantitative image analysis as an alternative to chemical analysis for follow-up of liver biopsies from a toucan with hemochromatosis. A technique with potential value for the follow-up of hemochromatosis in humans
Case Reports: 1996 Jun;18(3):221-4
Abstract: To compare the reliability of quantitative image analysis of histologic sections versus chemical analysis for determination of the iron content of serial liver biopsies taken at regular intervals after the onset of treatment, in a case of hemochromatosis in a channel-billed toucan treated with deferoxamine mesylate in combination with a low-iron diet. - K Pilegaard, O Ladefoged
Total number of astrocytes in the molecular layer of the dentate gyrus of rats at different ages
1996 Aug;18(4):279-85
Abstract: To estimate the total number of glial fibrillary acidic protein (GFAP)-positive astrocytes and the volume of GFAP-positive structures in the left molecular layer of the dentate gyrus by unbiased methods to provide reference values for neurotoxicologic model studies. - L Kusuda, M R Melamed
A new flow cytometric approach to the analysis of DNA and a second parameter
1996 Aug;18(4):309-15
Abstract: To describe a new method of analyzing flow cytometry data by continuously variable gating (CVG), which is particularly useful for analysis of cellular DNA plus a second parameter, such as the proliferation marker Ki-67. - P Karakitsos, B Cochand-Priollet, P J Guillausseau, A Pouliakis
Potential of the back propagation neural network in the morphologic examination of thyroid lesions
1996 Dec;18(6):494-500
Abstract: To investigate the potential of back propagation (BP) neural networks (NNs) in the discrimination of benign from malignant thyroid lesions. - R Montironi, R Pomante, L Diamanti, P W Hamilton, D Thompson, P H Bartels
Evaluation of prostatic intraepithelial neoplasia after treatment with a 5-alpha-reductase inhibitor (finasteride). A methodologic approach
1996 Dec;18(6):481-93
Abstract: To develop a methodology applicable to the morphologic study of the efficacy of finasteride on prostatic intraepithelial neoplasia (PIN), a putative precursor of prostate cancer. - H V Tuczek, P Fritz, P Schwarzmann, X Wu, G Mähner
Breast carcinoma. Correlations between visual diagnostic criteria for histologic grading and features of image analysis
1996 Dec;18(6):461-70
Abstract: To investigate the relevance of image analysis for grading breast carcinomas. - H Galera-Ruiz, H Galera-Davidson, M de Miguel-Rodríguez, J L Villar-Rodríguez, R González-Cámpora, M Salguero-Villadiego, E Salas-Herrero, A Fernández-Rodríguez
Value of ploidy pattern and nuclear texture in the diagnosis of primary hyperparathyroidism
1996 Feb;18(1):35-42
Abstract: To determine if there are subvisual differences (quantitation of DNA and study of chromatin texture) that distinguish single-gland parathyroid disease (adenoma) from multiple-gland disease (hyperplasia). - C A Beltrami, V Della Mea, N Finato, M Rocco
Computer-assisted morphometric analysis of the heart
1996 Apr;18(2):129-36
Abstract: To assess the applicability of the image analyzer for morphometric study of cardiomyopathies. - G A Meijer, J P Baak, P J van Diest, A H van Hattum, H C van der Linden, J J Koevoets
Text processing by digital voice recognition
1996 Aug;18(4):261-6
Abstract: To evaluate digital voice recognition in free text mode. - P W Hamilton, N H Anderson, J Diamond, P H Bartels, J B Gregg, D Thompson, R J Millar
An interactive decision support system for breast fine needle aspiration cytology
1996 Jun;18(3):185-90
Abstract: To develop a computerized system to assist in the diagnosis of malignancy in breast fine needle aspiration cytology. - K Kayser, S Berthold, S Eichhorn, C Kayser, S Ziehms, H J Gabius
Application of attributed graphs in diagnostic pathology
1996 Aug;18(4):286-92
Abstract: To compute attributed graphs based upon calculation of the minimum spanning tree (MST) for various applications in diagnostic lung pathology. - J A Giménez-Mas, P Gallego-Calvo, P Sanz-Moncasi, J Ríos-Mitchell, I Valero, M Sanz-Anquela, J Burriel, A Bavai
AgNOR evaluation by image processing methods. Staining modifications and results in 126 invasive ductal breast carcinomas
1996 Feb;18(1):9-18
Abstract: To analyze cellular proliferative activity in breast cancer by means of a silver (Ag) method (AgNOR) that stains the nucleolar organizer regions (NORs), structures involved in protein transcription. - R A Williams, J P Baak, G A Meijer, I G Charlton
Testing the value of a measurement protocol for DNA image cytometry and comparing different cytologic preparations
Comparative Study: 1996 Dec;18(6):429-37
Abstract: To analyze the influence of measuring parameters on the quality of DNA histograms, and to compare the results of DNA image cytometry (ICM) and flow cytometry (FCM). - L Desinan, C A Scott, S Pizzolitto, C Avellini, P Bardus, G Rimondi, V Rizzi, C A Beltrami
DNA flow cytometry and glial fibrillary acidic protein reactivity in pleomorphic adenomas of the salivary glands
1996 Oct;18(5):355-60
Abstract: To evaluate and correlate morphologic features, glial fibrillary acidic protein (GFAP) reactivity and DNA content parameters in 32 pleomorphic adenomas of the salivary glands. - R L Becker Jr, U V Mikel, W R Oliver, I A Sesterhenn
Enumeration of interphase chromosomes. Comparison of visual in situ hybridization and confocal fluorescence in situ hybridization
Comparative Study: 1996 Oct;18(5):405-9
Abstract: To compare two visual enumeration methods to determine whether the confocal approach yielded better counts of chromosome-specific hybridization sites. - H Kolles, A von Wangenheim, J Rahmel, I Niedermayer, W Feiden
Data-driven approaches to decision making in automated tumor grading. An example of astrocytoma grading
1996 Aug;18(4):298-304
Abstract: To compare four data-driven approaches to automated tumor grading based on morphometric data. Apart from the statistical procedure of linear discriminant analysis, three other approaches from the field of neural computing were evaluated. - R Roldán-Villalobos, E Artacho-Pérula, F J Ruiz-Moruno
Grading and prognosis of infiltrating ductal breast carcinoma by mean nuclear volume estimates
1996 Apr;18(2):158-66
Abstract: Quantitative evaluation of nuclear size in breast cancer is performed with the aim of investigating whether the data obtained are related to subjective grading and prognosis. - G A Meijer, P J van Diest, J P Baak, M Brinkhuis, M Beentjes
Influence of boundary effects on minimum spanning tree features. A computer simulation
1996 Jun;18(3):225-32
Abstract: To assess the influence of boundary effects on quantitative data derived from the minimum spanning tree (MST). - S Caulet-Maugendre, M Patey, E Granier, A Joundi, A Gentile, T Caulet
Quantitative analysis of cellular proliferative activity in 35 T-cell non-Hodgkin's lymphomas. Use of proliferating cell nuclear antigen and Ki-67 (MIB-1) antibodies and nucleolar organizer regions
Comparative Study: 1996 Oct;18(5):337-44
Abstract: To analyze cellular proliferation in non-Hodgkin's lymphoma (NHL) of T-cell origin using three variables available on histologic paraffin, dewaxed sections. - P J van Diest, D R Weger, J Lindholm
Reproducibility of subjective immunoscoring of steroid receptors in breast cancer
1996 Oct;18(5):351-4
Abstract: To evaluate the reproducibility of subjective immunoscoring of steroid receptors in breast cancer by inexperienced observers after brief training by one experienced observer. - A G Nikonenko
The cell sectioning model. Nuclear/cytoplasmic ratio studied by computer simulation
1996 Feb;18(1):23-34
Abstract: Stereologic analysis can be supported using methods of modeling and simulation. To illustrate the use of a mathematical cell sectioning model (CSM) in predicting two-dimensional (2-D) measurement characteristics of sectioned cells by means of computer-assisted simulation, the present study was performed. - K Kayser, K Baumgartner, H J Gabius
Cytometry with DAPI-stained tumor imprints. A reliable tool for improved intraoperative analysis of lung neoplasms
1996 Apr;18(2):115-20
Abstract: To determine the feasibility and potential use of application of 4'-6-diamidino-2-phenylindole dihydrochloride-stained imprints taken from primary lung carcinomas for intraoperative frozen section service. - R A Williams, J P Baak, G A Meijer, I G Charlton
Further testing of the value of a measurement protocol for DNA ploidy studies using image cytometry. Comparison of results obtained from cytologic and paraffin section preparations
Comparative Study: 1996 Oct;18(5):345-50
Abstract: To apply DNA image cytometry (ICM) to 53 tissue sections of breast cancer and skin melanomas. - P Bonara, M Solca, A Baisi, D Bauer, A Galli, C Mocellin, L Matturri
Flow cytometric analysis of bronchoalveolar lavage and venous blood lymphocyte phenotype for the diagnosis of acute graft rejection in lung transplant patients. The Lung Transplant Group, Ospedale Maggiore di Milano
Comparative Study: 1996 Aug;18(4):293-7
Abstract: To investigate modifications of phenotype in bronchoalveolar lavage (BAL) and venous blood lymphocytes as markers of acute organ rejection in lung transplant patients. - E Artacho-Pérula, A López-Beltrán, E Collantes-Estévez, R Roldán-Villalobos
Quantitative histopathologic discrimination of rheumatic disorders using nuclear measurements of synoviocytes
1996 Oct;18(5):420-8
Abstract: To use several nuclear quantitative estimators of synoviocytes and advanced statistical tests to discriminate between rheumatic disorders with the purpose of providing an objective histopathologic differentiation and to illustrate the difficulty of establishing the implications of the vague diagnosis of chronic nonspecific synovitis. - M Zaczek, W Szot, Z Chłap
Argyrophilic nucleolar organizer regions in proliferative lesions of the thyroid gland
1996 Feb;18(1):1-8
Abstract: To analyze various argyrophilic nucleolar organizer regions (AgNOR) parameters in proliferative lesions of the thyroid gland and to determine to what extent these parameters are influenced by the type of lesion or malignancy. - D Thompson, P H Bartels, H G Bartels, R Montironi
Knowledge-guided histometry of the basal cell layer in prostatic intraepithelial neoplasia
1996 Apr;18(2):121-8
Abstract: To define a procedure for the fully automated evaluation of histopathologic sections of prostatic intraepithelial neoplasia. - S Kneitz, G Ott, Albert, T Schindewolf, H K Müller-Hermelink, H Harms
Differentiation of low-grade non-Hodgkin's lymphoma by digital image processing
1996 Apr;18(2):177-84
Abstract: To identify different types of low-grade B-cell non-Hodgkin's lymphoma (NHL) classified according to the Revised European American Lymphoma and Kiel classification systems by means of digital image processing. - B Loehrke, U Renne, T Viergutz, K Ender, B Krüger
Effects of stress-related signal molecules on cells associated with muscle tissue
1996 Dec;18(6):471-80
Abstract: To elucidate the physiologic background that makes muscle hypertrophy, especially that due to strenuous exercise, often parallel to stress sensitivity and signs of acute phase immune response. - S M Rahman, H Itakura
Morphometry in histopathology. An image analysis workstation for the pathology laboratory
1996 Dec;18(6):461-70
Abstract: To investigate the value of morphometry in comparative histologic analysis of diseases, fixing a set of parameters suitable for analysis and to create a general strategy for data storage and economic data management. - G Kontogeorgos, N Kapranos, D Rologis, E Vamvakaris, N Papadopoulos
DNA measurement in pituitary adenomas assessed on imprints by image analysis
1996 Apr;18(2):144-50
Abstract: To determine the DNA content and S-phase fraction (SPF) of pituitary adenomas by image analysis and to correlate them with clinical and morphologic parameters. - A Sampedro, G Urdiales, A Martínez-Nistal, J Riera, D Hardisson
Prognostic value of DNA image cytometry in colorectal carcinoma
1996 Jun;18(3):214-20
Abstract: To investigate the diagnostic sensitivity and prognostic predicting of DNA image cytometry in colorectal carcinoma. - C Souchier, M P Guigard, P Fonlupt, J Trouillas
Quantitative evaluation of the distribution of cell density. Application to human pituitary corticotroph cells
1996 Jun;18(3):251-60
Abstract: An image analysis program was developed to analyze cell density in a whole organ. The method was applied to the evaluation of adrenocorticotrophic hormone (ACTH-storing cells in normal human anterior pituitary in order to help the pathologist to evaluate hyperplasia on small surgical biopsies. - E Kahn, J Hotmar, A Bernheim
Three-dimensional fluorescence in situ hybridization. Chromosomal studies on nuclei from cytogenetic preparations by confocal microscopy
1996 Apr;18(2):109-14
Abstract: To determine the three-dimensional (3D) position of target sequences and chromosomal volumes in interphase human nuclei by confocal laser scanning microscopy (CLSM) by using the heterochromatin part of the long arm of human chromosome Y (HPLAHC) as a target for specific DYZ1 probes, then by D10Z1 probes specific to the centromere of chromosome 10. - S K Sarker, R Mendiola, A Spigelman, M Walker, D Coleman
DNA ploidy on cytologic and tissue sections of breast lumps. A comparison using image analysis
Comparative Study: 1996 Feb;18(1):19-22
Abstract: Ploidy correlation between cytologic and histologic breast specimens would permit preoperative measurement of ploidy and may assist in patient management. - C Bertoni-Freddari, P Fattoretti, T Casoli, U Caselli, W Meier-Ruge
Deterioration threshold of synaptic morphology in aging and senile dementia of Alzheimer's type
1996 Jun;18(3):209-13
Abstract: To search for a deterioration threshold of synaptic ultrastructure in physiologic aging and senile dementia of Alzheimer's type (SDAT). - J Smolle
Optimization of linear image combination for segmentation in red-green-blue images
1996 Aug;18(4):323-9
Abstract: To present an iterative algorithm that selects the optimal pair of grey level image components of red-green-blue (RGB) and hue-luminance-saturation (HLS) color image models and the optimal weighting factor for automated segmentation of the structure of interest in color images. - L J Elavathil, G Celebre, D McFarlane
Reproducibility of DNA ploidy and S-phase values from paraffin-embedded tissue
1996 Aug;18(4):316-22
Abstract: To determine the degree of variability of DNA flow cytometric results between two types of flow cytometers. - T Mairinger, A Gschwendtner, M Toetsch, C Prior, F Fend
Automated cell differentiation of bronchoalveolar lavage samples with two-step image analysis
1996 Dec;18(6):453-60
Abstract: To establish an easy method for performing an automated differential cell count on bronchoalveolar lavage (BAL) cytocentrifuge samples using morphometric parameters acquired by a digital image analyzer. - G Haroske, V Dimmer, D Steindorf, U Schilling, F Theissig, K D Kunze
Cellular sociology of proliferating tumor cells in invasive ductal breast cancer
1996 Jun;18(3):191-8
Abstract: The topology of proliferating cells in tissue sections of breast cancers was studied as the static expression of the cellular sociology of distinct subpopulations of tumor cells. With the terms neighborhood and connectivity, an attempt was made to describe the mutual interactions of cycling tumor cells objectively in different prognostic subgroups of invasive ductal breast carcinomas. - J Azúa, P Romeo, J Valle, J Azúa Jr
DNA quantification as a prognostic factor in prostatic adenocarcinoma
1996 Aug;18(4):330-6
Abstract: To determine if DNA quantification on fine needle aspiration (FNA) has a predictive value in cancer of the prostate. - A Srivastava, P K Agarwal, M M Goel, S Tandon, M Gupta, R P Misra
Observer variation in AgNOR counts in neoplastic breast lesions
1996 Jun;18(3):241-4
Abstract: To determine interobserver and intraobserver variability of AgNOR quantitation in neoplastic lesions of the breast. - L Desinan, C A Scott, S Pizzolitto, C Avellini, G Rimondi, P Bardus, V Rizzi, G Talmassons, C Puricelli, C A Beltrami
Non-small cell lung cancer. Morphology and DNA flow cytometry
1996 Aug;18(4):275-8
Abstract: To correlate stage-related and histologic features of non-small cell lung cancer (NSCLC) with DNA flow cytometric parameters. - C Bertoni-Freddari, P Fattoretti, U Caselli, R Paoloni
Acetylcarnitine modulation of the morphology of rat hippocampal synapses
1996 Aug;18(4):261-6
Abstract: To measure the effect of chronic administration of acetylcarnitine (ALCAR) on the morphologic plasticity of rat hippocampal synapses.