Volume 11 (1995) Issue 1 - Issue 6
- D Thompson, P H Bartels, H G Bartels, R Montironi
Image segmentation of cribriform gland tissue
1995 Oct;17(5):314-22
Abstract: To develop procedures for the segmentation of cribriform prostatic glands. A knowledge-guided procedure following a model-based reasoning process was developed in the context of a set of interacting expert systems for machine vision in histometry. - A Böcking, F Giroud, A Reith
Consensus report of the European Society for Analytical Cellular Pathology task force on standardization of diagnostic DNA image cytometry
Review: 1995 Feb;17(1):1-7 - K L Chua, A Hjerpe
Polymerase chain reaction analysis of human papillomavirus in archival cervical cytologic smears
1995 Aug;17(4):221-9
Abstract: We used a nested polymerase chain reaction (PCR) to study the presence of human papillomavirus (HPV) DNA in stained, archival cervical cytologic smears, where MY09/MY11 served as outer primers and GP5/GP6 as inner primers. - J A Giménez-Mas, M P Sanz-Moncasi, L Remón, P Gambó, M P Gallego-Calvo
Automated textural analysis of nuclear chromatin. A mathematical morphology approach
1995 Feb;17(1):39-47
Abstract: Nuclear grading of neoplasms has classically been involved in prognosis and must be established by combining different parameters, such as the textural pattern of chromatin, which is subjective and difficult to measure. - G Fadda, C Rabitti, C Minimo, A Ieraci, A Verzì, A Bianchi, M Lancia, G Gullotta, A Capelli
Morphologic and planimetric diagnosis of follicular thyroid lesions on fine needle aspiration cytology
1995 Aug;17(4):247-56
Abstract: During 1989-1992, 2,729 fine needle aspiration biopsies were performed; 585 with histologic controls were reviewed. The aim of the study was to demonstrate the incidence of carcinoma in follicular-structured smears without nuclear enlargement, evaluated with planimetric techniques, and to suggest a new cytodiagnostic classification. - R Stotzka, R Männer, P H Bartels, D Thompson
A hybrid neural and statistical classifier system for histopathologic grading of prostatic lesions
1995 Jun;17(3):204-18
Abstract: Neural network and statistical classification methods were applied to derive an objective grading for moderately and poorly differentiated lesions of the prostate, based on characteristics of the nuclear placement patterns. - W H Wolberg, W N Street, O L Mangasarian
Image analysis and machine learning applied to breast cancer diagnosis and prognosis
1995 Apr;17(2):77-87
Abstract: Fine needle aspiration (FNA) accuracy is limited by, among other factors, the subjective interpretation of the aspirate. We have increased breast FNA accuracy by coupling digital image analysis methods with machine learning techniques. - E Piaton, P P Bringuier, D Seigneurin, P Perrin, M Devonec
Flow and image cytometric DNA measurements in fine needle aspiration samples of prostatic neoplasms
Comparative Study: 1995 Oct;17(5):351-60
Abstract: To compare the DNA content measured by flow cytometry (FCM) and image analysis (IA) from prostatic fine needle aspiration (FNA) samples. A total of 48 samples were studied. FCM was performed on propidium iodide-stained nuclei according to the Vindelov method, and image analysis was performed on Feulgen-stained slides. Positive FNA results were grade (1-3) and compared with Gleason grades. - B Susnik, A Worth, J LeRiche, B Palcic
Malignancy-associated changes in the breast. Changes in chromatin distribution in epithelial cells in normal-appearing tissue adjacent to carcinoma
1995 Feb;17(1):62-8
Abstract: Malignancy-associated changes (MACs) have been described as subtle morphologic changes in normal-appearing tissues adjacent to or distant from malignant tumors. MACs might be applicable as a marker of occult malignancy, increased risk of subsequent carcinoma or recurrence of tumor after therapy. - K Kayser, J Bubenzer, G Kayser, S Eichhorn, T V Zemlyanukhina, N V Bovin, S Andre, J Koopmann, H J Gabius
Expression of lectin, interleukin-2 and histopathologic blood group binding sites in prostate cancer and its correlation with integrated optical density and syntactic structure analysis
Comparative Study: 1995 Apr;17(2):135-42
Abstract: The binding of several biotinylated biologic probes was determined in sections of 20 surgical specimens of prostate cancer and of 21 biopsy specimens of hyperplastic prostate. - N Poulin, B Susnik, M Guillaud, A Doudkine, A Worth, B Palcic
Histometric texture analysis of DNA in thin sections from breast biopsies. Application to the detection of malignancy-associated changes in carcinoma in situ
1995 Oct;17(5):291-9
Abstract: To evaluate histometric measurement of nuclear texture in breast biopsy sections in order to detect malignancy-associated changes in apparently normal tissue in the vicinity of carcinoma in situ. We previously showed that image cytometry measurements of nuclear features--foremost, texture features, describing the organization of Feulgenstained DNA in the cell--can be used to distinguish normal-appearing, diploid epithelial cells from patients with invasive carcinoma of the breast from those with benign biopsies. - M Brinkhuis, G A Meijer, J A Beliën, P J van Diest, J P Baak
Volume-weighted mean nuclear volume and nuclear area in advanced ovarian carcinoma. An investigation of sampling methods, sample size and reproducibility
1995 Aug;17(4):284-90
Abstract: The influence of sampling issues on the reproducibility of volume-weighted mean nuclear volume (mean v) and mean nuclear area (MNA) assessments in patients with International Federation of Gynecology and Obstetrics stage III and IV ovarian carcinoma was evaluated. - T J Filler, C H Rickert, B Zhao
Morphologic quantification of blood platelets by image analysis
1995 Dec;17(6):361-5
Abstract: For morphologic and functional investigations on the physiology and pathology of human blood platelets, their adhesion plays an important role. This feature is normally assessed concerning qualitative aspects, but there is a lack of quantitative investigations. - M J Kline, E J Wilkinson, R Askeland, R W Given, C Stephen, J B Hendricks
DNA tetraploidy in Feulgen-stained bladder washings assessed by image cytometry
1995 Apr;17(2):129-34
Abstract: The prognostic utility of DNA cytometry has been demonstrated for irrigation specimens from bladder neoplasms. While the traditional method of measuring the DNA content of cells recovered by bladder irrigation is flow cytometry, image analysis has been applied increasingly, with successful results. - B Lifson, J G Pizzolo, A A Elhosseiny, M S Choudhury, M R Melamed
Comparison of fluorescence in situ hybridization and flow cytometric DNA ploidy analysis in paraffin-embedded prostatic adenocarcinoma specimens
Comparative Study: 1995 Apr;17(2):93-9
Abstract: Cancer cell nuclei extracted from 15 paraffin-embedded radical prostatectomy specimens were examined with fluorescent in situ hybridization (FISH) and flow cytometry (FCM) for DNA ploidy. Repetitive chromosome-specific alpha-satellite DNA probes to centromeres 7 and 10 were used in the FISH assay. - H J Carson, R J Buschmann, J Cintron, P Weisz-Carrington
Prognostic histomorphometry in colorectal adenocarcinoma
1995 Apr;17(2):109-12
Abstract: In order to examine the use of morphometry in the prognosis of colorectal adenocarcinoma, histologic material from 30 patients was analyzed. - P W Hamilton, P H Bartels, R H Wilson, J M Sloan
Nuclear texture measurements in normal colorectal glands
1995 Dec;17(6):397-405
Abstract: To examine differences in epithelial cell nuclear texture along the length of normal, well-oriented colorectal glands. - I Salmon, S Rorive, I Camby, C Decaestecker, B Pirotte, K Rombaut, J Haot, J L Pasteels, J Brotchi, R Kiss
Stereotactic biopsies from astrocytic tumors. Diagnostic information contributed by the quantitative chromatin pattern description
1995 Oct;17(5):332-43
Abstract: To reduce the problem of heterogeneity in astrocytic tumors by means of computer-assisted microscope analysis of Feulgen-stained nuclei. - B Susnik, N Poulin, D Phillips, J LeRiche, B Palcic
Comparison of DNA measurement performed by flow and image cytometry of embedded breast tissue sections
Comparative Study: 1995 Jun;17(3):163-71
Abstract: Tissue sections are important for morphometric studies, such as the analysis of architectural pattern of tissues as well as nuclear morphology and chromatin texture describing DNA distribution in nuclei. Tissue sections are generally not recommended for use in DNA ploidy measurements due to possible errors based on sectioned and overlapped nuclei. - P Kronqvist, R Montironi, Y U Collan, P H Bartels, D Thompson
Management of uncertainty in breast cancer grading with Bayesian belief networks
1995 Oct;17(5):300-8
Abstract: To examine the potential of different constructs of Bayesian belief networks (BBN) to manage uncertainty in breast cancer grading. - J Pfisterer, F Kommoss, W Sauerbrei, D Menzel, M Kiechle, E Giese, M Hilgarth, A Pfleiderer
DNA flow cytometry in node-positive breast cancer. Prognostic value and correlation with morphologic and clinical factors
1995 Dec;17(6):406-12
Abstract: To help clarify the possible usefulness of nuclear DNA content and S-phase fraction (SPF) as additional prognostic factors in node-positive breast cancer patients because there is increased interest in the development of new factors that might provide more detailed prognostic information. - M García-Bonafé, A Moragas
Nuclear texture in poorly differentiated small round cell tumors. Image analysis study of fine needle aspiration material
1995 Jun;17(3):189-96
Abstract: Gradient analysis and pattern spectrum decomposition based on mathematical morphology concepts were used to explore nuclear texture patterns in a pool of 108 cells obtained by fine needle aspiration of five undifferentiated small round cell tumors of childhood, including one case each of Wilms' tumor, neuroblastoma, lymphoblastic lymphoma, Ewing's sarcoma and rhabdomyosarcoma. - F Ballouk, A Boguniewicz, J S Ross, T A Jennings
Image analysis quantification in hormone receptor assay of mucinous carcinoma of the breast. Comparison with biochemical analysis
Comparative Study: 1995 Jun;17(3):151-6
Abstract: Mucinous carcinoma (MC) of the breast is characterized by abundant extracellular mucin and by variable epithelial cellularity. Since some MCs are extremely hypocellular, we questioned the validity of biochemical (BIO) assays in these tumors. - R Bowman, Z A Kanacki, C Garberoglio, D R Chase
Comparison between image and flow cytometry. A priori factors that influence technique
Comparative Study: 1995 Aug;17(4):276-83
Abstract: Comparisons between flow cytometry (FCM) and image cytometry (ICM) have found a high concordance rate in pancreatic tissue, with some discrepancies between the two procedures. - J G Cowpe, G R Ogden, M Thompson
Comparison of nuclear area measurements carried out on normal and dysplastic oral smears
Comparative Study: 1995 Aug;17(4):241-6
Abstract: We previously showed that quantitative cytology can help to detect oral cancer, not only at initial presentation but also in patients with unstable mucosa in whom recurrence has been detected prior to detection of a clinically obvious cancer. - C Midulla, M R Giovagnoli, C Valli, A Vecchione
Correlation between ploidy status, Erb-B2 and p53 immunohistochemical expression in primary breast carcinoma
1995 Jun;17(3):157-62
Abstract: In the present study we evaluated the prognostic significance and correlation between ploidy status, lymph node status, estrogen-progesterone receptor status and the expression of Erb-B2 and p53 protein in 77 primary breast carcinomas. - W H Wolberg, W N Street, D M Heisey, O L Mangasarian
Computer-derived nuclear "grade" and breast cancer prognosis
1995 Aug;17(4):257-64
Abstract: Visual assessments of nuclear grade are subjective yet still prognostically important. Now, computer-based analytical techniques can objectively and accurately measure size, shape and texture features, which constitute nuclear grade. - C MacAulay, S Lam, P W Payne, J C LeRiche, B Palcic
Malignancy-associated changes in bronchial epithelial cells in biopsy specimens
1995 Feb;17(1):55-61
Abstract: A total of 152 normal bronchial biopsy sections--63 from normal subjects, 42 from patients with dysplasia, 28 from patients with early or advanced lung cancer with squamous and nonsquamous histopathology, and 19 from resected lung cancer patients--were examined for the presence of malignancy-associated changes (MACs). - N Ikeda, C MacAulay, S Lam, D M Garner, P W Payne, H Kato, C Konaka, B Palcic
Use of high-resolution cytometry in predicting the biologic behavior of T1 adenocarcinoma of the lung
1995 Feb;17(1):69-74
Abstract: Touch preparations from 60 cases of T1 adenocarcinoma were analyzed using a high-resolution, automated image cytometer. These cases were divided according to pathologic stage: stage I, 31; stage II, 3; stage III, 19; and stage IV, 7. - M L Ostrowski, S Chakraborty, R Laucirica, R W Brown, S D Greenberg
Quantitative image analysis of MIB-1 immunoreactivity. A comparison with flow cytometric assessment of proliferative activity in invasive carcinoma of the breast
Comparative Study: 1995 Feb;17(1):15-24
Abstract: Proliferative activity, especially flow cytometrically determined S-phase fraction, is generally accepted as an important prognostic indicator in carcinoma of the breast. We studied cellular proliferation in 53 breast carcinomas using quantitative image analysis of immunoreactivity to a recently available monoclonal antibody, MIB-1, which is applicable to formalin-fixed, paraffin-embedded tissues. - L A Krogerus, M Railo, M Schoultz, S Nordling
Flow cytometric DNA measurements in aspiration biopsies and surgical specimens of breast cancer
1995 Oct;17(5):309-13
Abstract: One of the prognostic factors in breast cancer is the proliferation activity of the tumor. This study sought knowledge of this activity before surgery to benefit the design and timing of therapy. - R K Brouwer, C MacAuley
Classifying cervical cells using a recurrent neural network by building basins of attraction
1995 Jun;17(3):197-203
Abstract: This paper describes the result of classifying cervical cells employing a novel way of using a Hopfield-style neural network for classification. This method could be used as part of an automated cervical screening system. - R C Callaghan, C Carda, A Peydró-Olaya, T Triche, A Llombart-Bosch
Small round cell tumors of bone and soft tissue. A morphometric and stereometric comparative analysis of 119 cases
Comparative Study: 1995 Dec;17(6):374-82
Abstract: To analyze the discriminative capability of morphometric assessment of nuclear morphology in the differential diagnosis of small round blue cell tumors (SRCTs) of bone and soft tissue. - E Leo, M Kropff, A Lindemann, G Steinfurth, H Esselborn, R Rossner, C P Adler, A Böcking
DNA aneuploidy, increased proliferation and nuclear area of plasma cells in monoclonal gammopathy of undetermined significance and multiple myeloma
Comparative Study: 1995 Apr;17(2):113-20
Abstract: DNA cytometric and morphometric parameters of plasma cells were determined in 73 multiple myelomas (MMs), 31 monoclonal gammopathies of undetermined significance (MGUS), 11 reactive plasmacytoses (RPs) and 33 cases of normal bone marrow (NBM) using a TV-based static cytometer combined with a computerized relocation stage. - P D Unger, V Hoon, N Stone, Z Liu, N Theise, R Stock, J Gil
Computerized interactive morphometry in the differential diagnosis of irradiated prostates
1995 Apr;17(2):100-8
Abstract: Radiation therapy is becoming a treatment of choice for many patients with prostatic carcinoma. Distinguishing radiation change in prostate glands from carcinoma may be difficult. - L Qiao, W Gorczyca, J G Pizzolo, M R Melamed, Z Darzynkiewicz
Flow cytometric characterization of proliferation-associated nuclear antigen (p105) during the cell cycle in normal lymphocytes and promyelocytic leukemia cells (HL-60)
1995 Jun;17(3):183-8
Abstract: Bivariate flow cytometric analysis of p105 expression and DNA content was performed in human lymphocytes and promyelocytic leukemia cells (HL-60). We also employed a new method of detecting DNA strand breaks associated with apoptosis by labeling the 3'-OH termini in the breaks with biotinylated dUTP in a reaction employing exogenous terminal deoxynucleotidyl transferase (TdT). - C A Beltrami, V Della Mea, N Finato
Structure analysis of breast lesions using neighborhood graphs
1995 Apr;17(2):143-50
Abstract: The College of American Pathologists divides proliferative lesions of the breast into three categories, but the diagnostic criteria for classification are not easy to apply. Therefore, many morphologic, immunohistochemical and morphometric features were studied in order to clarify this subdivision; however, the reported results are not very satisfactory. - C Charpin, C Allasia, J D Davies, B Devictor, J Boulat, J L Ansaldi, M N Lavaut, B Serradour, L Andrac, L Piana
Digitization of microcalcifications in breast radiographs. Correlation with pathologic data
Comparative Study: 1995 Aug;17(4):230-40
Abstract: A series of 104 nonpalpable breast lesions detected by mammograms and containing microcalcifications were studied. Intraoperative radiographs of intact and sliced specimens were assessed, followed by microscopic diagnosis on frozen sections of areas containing microcalcifications. - P W Hamilton, D Thompson, J M Sloan, P H Bartels
Knowledge-guided segmentation and morphometric analysis of colorectal dysplasia
1995 Jun;17(3):172-82
Abstract: The histopathologic grading of colorectal adenomatous dysplasia is a subjective process. In this study, automated image analysis using knowledge-guided software was used to quantitatively assess colorectal glandular characteristics. - M Beil, T Irinopoulou, J Vassy, J P Rigaut
Chromatin texture analysis in three-dimensional images from confocal scanning laser microscopy
1995 Oct;17(5):323-31
Abstract: Three-dimensional (3D) images of tissue blocs can be obtained through optical sectioning by a confocal scanning laser microscope and by piling up of consecutive confocal planes. These images offer the opportunity to quantitate biologic structures (i.e., nuclear chromatin) in 3D. Chromatin texture analysis is regarded as a key element in nuclear grading of malignant lesions. - P Herlin, F Duigou, E Masson, J Marnay, J L Liu, M Henry-Amar, D Bloyet, J C Mandard, A M Mandard
DNA cytometric abnormalities in human esophageal squamous intraepithelial and invasive carcinomas
Comparative Study: 1995 Feb;17(1):8-14
Abstract: DNA ploidy abnormalities of 21 archival human esophageal intraepithelial neoplasia samples were assessed, using image cytometry of deparaffinized samples, with reference to invasive squamous cell carcinoma and corresponding uninvolved squamous epithelium. - G R Ogden, D M Chisholm, M Green, J G Cowpe, E B Lane
Influence of temperature on long-term keratin immunoreactivity for oral exfoliative cytology
1995 Feb;17(1):35-8
Abstract: We previously showed that keratin profiles can be of value in the diagnosis of oral cancer when using exfoliative cytology. In the future, they may form part of a screening program for oral cancer. This study evaluated the influence of long-term storage on keratin expression. - J B Hendricks, R Rainer, S Munakata
Computer-assisted and visual methods of assessing cellular proliferation in tissue sections from non-Hodgkin's lymphoma a comparison
1995 Dec;17(6):383-8
Abstract: To compare computer-assisted and visual methods of assessing cellular proliferation using tissue sections from non-Hodgkin's lymphoma (NHL). - L J Van Bogaert
Immunohistochemical detection and significance of axillary lymph node micrometastases in breast carcinoma
Comment: 1995 Feb;17(1):75-6 - C Robert, C Fouchet, C Vergely, D Pruneau, P Bélichard
Quantitative image analysis of cell proliferation after balloon catheter injury in the rabbit carotid artery
1995 Dec;17(6):366-73
Abstract: To perform color image analysis to assess the course of morphometric and proliferative changes in the intima and media of rabbit carotid arteries following balloon injury. - P Dey T Karmakar
Quantitative estimation of mast cells in Hodgkin's disease and reactive lymphoid/hyperplasia in aspiration cytology smears
1995 Jun;17(3):219-20 - I Papadopoulos, K Weichert-Jacobsen, N Nürnberg, E Sprenger
Quantitative DNA analysis in renal cell carcinoma. Comparison of flow and image cytometry
Comparative Study: 1995 Aug;17(4):272-5
Abstract: In 70 renal cell carcinomas, nuclear DNA content was determined by means of flow cytometry (FCM) and image cytometry (ICM). The two methods produced comparable results as to DNA tumor ploidy (DNA tumor stemlines, DNA index): 14 of the tumors were tetraploid or aneuploid and 56 diploids. - L A Talve, Y U Collan, K A Alanen
Bleaching of melanin before image cytometry of the DNA content of pigmented skin tumors
1995 Oct;17(5):344-50
Abstract: To study the effect of bleaching of melanin with KMnO4 on the results of DNA image cytometry in pigmented skin tumors. - J V Soames, R I Macleod
Effects of radiotherapy on oral epithelium. Quantitative cytology using light and scanning electron microscopy
1995 Dec;17(6):389-96
Abstract: To determine the short- and long-term morphologic and morphometric effects of radiotherapy on exfoliated oral squames. - G H Yu, N Sneige, L D Kidd, D A Johnston, R L Katz
Image analysis--derived morphometric differences in fine needle aspirates of ductal and lobular breast carcinoma
1995 Apr;17(2):88-92
Abstract: The differentiation of ductal and lobular carcinoma (including lobular carcinoma in situ) of the breast has important prognostic implications; thus, preoperative differentiation of these entities via fine needle aspiration could have a substantial impact on the therapeutic options offered to patients. - B Bucci, V Pansadoro, F De Paula, A Florio, E Carico, G Zupi, A Vecchione
Biologic characteristics of T1 papillary bladder cancer. Flow cytometric study of paraffin-embedded material
1995 Apr;17(2):121-8
Abstract: DNA ploidy, proliferative activity (evaluated in terms of the proliferative index [PI]) and epidermal growth factor receptors (EGF-R) expression in primary bladder cancer in 52 patients were studied by means of flow cytometry and immunohistochemistry. - T M Goud, B S Dwarakanath, M Augustus, K M Bhargava
Flow cytometry of breast tumors. Relevance to clinicopathology and survival
1995 Aug;17(4):265-71
Abstract: Nuclear DNA content and cell cycle distribution in fresh tissues from 40 malignant and 10 benign breast tumors were assessed by flow cytometry using a DNA-specific fluorochrome, 4,6-diamidino-2phenyl-indole hydrochloride. - H Truong, R Morimoto, A E Walts, B Erler, A Marchevsky
Neural networks as an aid in the diagnosis of lymphocyte-rich effusions
1995 Feb;17(1):48-54
Abstract: Neural network (NN) technology was applied to digital image analysis data for 112 Papanicolaou-fixed and -stained smears of lymphocyte-rich effusions (LREs). The smears were analyzed with an inexpensive image analysis system assembled in our laboratory. - G Wolf, M Beil, H Guski
Chromatin structure analysis based on a hierarchic texture model
1995 Feb;17(1):25-34
Abstract: The quantification of chromatin structures is an important part of nuclear grading of malignant and premalignant lesions. In order to achieve high accuracy, computerized image analysis systems have been applied in this process.