Volume 10 (1994) Issue 1 - Issue 6
- M García-Bonafé, A Moragas, J de Gracia
Image analysis of hemosiderin-laden macrophages in bronchoalveolar lavage fluid
1994 Feb;16(1):11-7
Abstract: The aim of this study was to assess quantitatively the relationship between the number of iron-laden macrophages, extent of hemosiderin content and amount of dark-stained hemosiderin clumps in bronchoalveolar lavage (BAL) fluids. - F Gilles, A Gentile, V Le Doussal, F Bertrand, E Kahn
Grading of cystosarcoma phyllodes by texture analysis of tissue architecture
Comparative Study: 1994 Apr;16(2):95-100
Abstract: Conventional histologic grading of cystosarcoma phyllodes of the breast has not been entirely successful in the prognosis of recurrence or metastasis. Our study first developed a tumor grade classification based on computerized texture features and then compared the classification to conventional grading of these tumors. - L Antonangelo, P H Saldiva, E Amaro Júnior, V L Capelozzi
Utility of computerized morphometry combined with AgNOR staining in distinguishing benign from malignant pleural effusions
1994 Aug;16(4):247-52
Abstract: A study was designed to evaluate the usefulness of computer-aided image analysis in distinguishing benign from malignant pleural effusions. - S H Kuo, Y C Lee, Y R Liu, Y T Lee, K T Luh, S W How
Correlation of flow cytometric and microdensitometric DNA content analysis in lung cancer
1994 Feb;16(1):25-8
Abstract: The DNA histograms of 29 histologically verified primary lung cancers were analyzed with flow cytometry and microdensitometry. Fresh tumor tissues were imprinted on the slide and air dried for modified Feulgen staining. - M Scarpelli, P H Bartels, R Montironi, C M Galluzzi, D Thompson
Morphometrically assisted grading of astrocytomas
1994 Oct;16(5):351-6
Abstract: Alcohol-fixed, toluidine-blue-stained smears from 24 astrocytomas (12 low grade and 12 high grade or anaplastic) were included in the study. In each case 50 nuclei from representative areas of the tumor were selected for analysis; quantitative features pertaining to both the entire nucleus and the nucleoli were computed. - H M Kvasnicka, J Thiele, T Amend, R Fischer
Three-dimensional reconstruction of histologic structures in human bone marrow from serial sections of trephine biopsies. Spatial appearance of sinusoidal vessels in primary (idiopathic) osteomyelofibrosis
1994 Jun;16(3):159-66
Abstract: Computer-based three-dimensional (3D) reconstruction of histologic features permits the assessment of the spatial arrangement of complex structures and has the additional advantage of demonstrating aspects that may be obscured in conventional, two-dimensional evaluations. - R G Steinbeck, K M Heselmeyer, G U Auer
DNA ploidy in human colorectal adenomas
1994 Jun;16(3):196-202
Abstract: The DNA content of 101 colorectal adenomas of various histologic types resected from 83 patients was determined by image cytometric measurements in order to investigate if a correlation between DNA ploidy and particular histomorphologic features exists and if DNA measurements can be of additional diagnostic value. - Q Zhu, P Tekola, J P Baak, J A Beliën
Measurement by confocal laser scanning microscopy of the volume of epidermal nuclei in thick skin sections
1994 Apr;16(2):145-52
Abstract: The mean volume and shape of nuclei assessed in standard tissue sections by means of stereologic and morphometric methods are associated with prognosis in tumors of different sites. - M Bibbo, J Xiao, R Christen, B Fitzpatrick, H Galera-Davidson, P H Bartels, C Minimo
Use of computer graphic filters for the nuclear grading of hematoxylin and eosin-stained specimens from prostatic lesions
1994 Jun;16(3):183-8
Abstract: An inexpensive workstation is being developed to assist pathologists in diagnosing routine hematoxylin and eosin-stained slides. A linear discriminant model was applied to karyometric features of prostate lesions, and a grade according to Mostofi was determined from the discriminant values. - C Minimo, H Galera-Davidson, J Xiao, R Christen, B Fitzpatrick, P H Bartels, M Bibbo
Importance of different nuclear morphologic patterns in grading prostatic adenocarcinoma. An expanded model for computer graphic filters
1994 Oct;16(5):307-14
Abstract: The aim of this work was to continue the development of an interactive workstation for the nuclear grading of prostatic lesions by including a large range of nuclear patterns. - P Zeppa, G Ferrara, R Chiacchio, F Fulciniti, G Troncone, O Nappi, A Vetrani, L Palombini
L-thyroxine effects on thyreocytes and Hürthle cells in nodular hyperplastic goiter on fine needle aspiration samples. Morphometric evaluation
1994 Jun;16(3):189-95
Abstract: A morphometric study was performed on the thyreocytes and Hürthle cells in 20 cases of nodular hyperplastic goiter (NHG) and in 8 cases of Hürthle cell-rich NHG. - L Qiao, P Wu, A H Ghaleb, J G Pizzolo, T B Miller, M R Melamed
Bivariate flow cytometric analysis of p53 and DNA content in hepatocellular carcinoma
1994 Apr;16(2):124-30
Abstract: Bivariate flow cytometric analysis of p53 protein and DNA content was studied in archival specimens of hepatocellular carcinoma (HCC) from Chinese patients and corresponding benign liver tissues from a series of 51 patients at Sun Yat-sen University of Medical Sciences. - S H Kuo, M Y Lai, Y R Liu, Y T Lee, D S Chen, C S Lee, H C Hsu
Nuclear area and DNA content in tumor and nontumor portions of hepatocellular carcinoma
Comparative Study: 1994 Apr;16(2):153-8
Abstract: The nuclear area and DNA content between tumor and nontumor portions of hepatocellular carcinoma (HCC) were compared. Thirty pairs of imprint smears were made from surgical specimens and stained with a modified Feulgen reaction. - R Montironi, P H Bartels, D Thompson, M Scarpelli, P W Hamilton
Prostatic intraepithelial neoplasia. Development of a Bayesian belief network for diagnosis and grading
1994 Apr;16(2):101-12
Abstract: The diagnosis and grading of prostatic intraepithelial neoplasia (PIN) are affected by uncertainties that arise from the fact that almost all our knowledge of PIN histopathology is not expressed in numeric form but rather in descriptive linguistic terms, words and concepts. - S Pulczynski, O M Jensen
Quantitation of immunogold with an interactive image analysis system. A new, practical approach to antibody-induced modulation, internalization and intracellular transport of surface antigens in viable hematopoietic cells
1994 Dec;16(6):393-9
Abstract: This work aimed to evaluate the value of computerized quantitative analysis in ultrastructural studies on internalization and intracellular transport of leukocyte antigens traced with immunogold in viable lymphoid cells. - R J Sklarew, R Wali, M Auger, L D Kidd, R L Katz
Estrogen receptor immunostaining patterns imaged in breast cancer fine needle aspirates
1994 Aug;16(4):293-305
Abstract: Fine needle aspirates (FNA) after estrogen receptor (peroxidase-antiperoxidase) immunostaining were imaged using a SAMBA system. Integrated optical density (IOD) and mean optical density (MOD) was measured in individual nuclei. - F Tezuka, R Chiba, T Takahashi
Morphometric and multivariate statistical detection of cancer cells in endometrial cytology
1994 Oct;16(5):332-8
Abstract: Endometrial carcinoma was discriminated in cytologic studies using morphometric and multivariate statistical methods. On aspirated samples from 70 cases (10 well-differentiated adenocarcinomas, 4 hyperplasias and 56 normal controls), clumps of epithelial cells that could be regarded as the most diagnostically relevant were selected in each case. - M Aubele, G Burger, K Rodenacker
Problems concerning the quality of DNA measurements on Feulgen-stained imprints. A study of five fixation techniques
Comparative Study: 1994 Jun;16(3):226-32
Abstract: Feulgen-stained rat liver imprints were investigated, and hepatocytes, lymphocytes and granulocytes were measured. Additionally, chicken erythrocytes placed on the slides were measured as an external DNA standard. - M L Mello, A F Chambers
Image analysis of Feulgen-stained transformed NIH 3T3 cells differing in p21 expression and ras-induced metastatic ability
1994 Apr;16(2):113-23
Abstract: Nuclear phenotypes of clonal and polyclonal T24 H-ras-transformed NIH 3T3 cells differing in p21 expression and experimental metastatic ability were studied in Feulgen-stained preparations by image analysis. - F Kommoss, J Pfisterer, T Idris, E Giese, W Sauerbrei, W Schäfer, M Thome, A Pfleiderer
Steroid receptors in carcinoma of the breast. Results of immunocytochemical and biochemical determination and their effects on short-term prognosis
1994 Jun;16(3):203-10
Abstract: A group of 241 nonpretreated breast carcinomas was studied for estrogen receptor (ER) and progesterone receptor (PR) content by both immunocytochemistry (ICC) and biochemical analysis (dextran-coated charcoal [DCC]). - K Kayser, F Liewald, K Kremer, M Tacke, M Storck, P Faber, P Bonomi
Alteration of integrated optical density and intercellular structure after induction chemotherapy and survival in lung carcinoma patients treated surgically
1994 Feb;16(1):18-24
Abstract: Histologic sections of formalin-fixed, paraffin-embedded tissue consisting of 53 surgical specimens, 48 biopsy specimens and 29 intrapulmonary lymph nodes of human non-small cell lung carcinoma were Feulgen stained. - R S Cajulis, G K Haines 3rd, D Frias-Hidvegi, K McVary
Interphase cytogenetics as an adjunct in the cytodiagnosis of urinary bladder carcinoma. A comparative study of cytology, flow cytometry and interphase cytogenetics in bladder washes
Comparative Study: 1994 Feb;16(1):1-10
Abstract: To determine the role of interphase cytogenetics as an adjunct in the cytodiagnosis of transitional cell carcinoma (TCC) in bladder washes, 40 separate samples were prospectively evaluated by conventional cytology, flow cytometry (FCM) and interphase cytogenetics using fluorescence in situ hybridization (FISH) to detect numerical chromosomal abnormalities involving chromosomes 8 and 12. - R Ricco, G Serio, D M Caniglia, A Cimmino, T Lettini, A Lozupone, V P Delfino
Size and shape evaluation of astrocytoma nuclei with the shape analytical morphometry software system
1994 Oct;16(5):345-50
Abstract: Eighteen cases of astrocytomas diagnosed as grades 1-3 of malignancy were studied with analytical morphometry to determine if different grades can be discriminated by size and shape parameters related to the nuclei. - H R Anderson, A W Stitt, T A Gardiner, D B Archer
Estimation of the surface area and volume of the retinal capillary basement membrane using the stereologic method of vertical sections
1994 Aug;16(4):253-60
Abstract: The modern stereologic method of vertical sections was applied to the retina as a means of generating unbiased estimates of three-dimensional structure. - A Russo, V Bazan, V Morello, V Tralongo, C Nagar, R Nuara, G Dardanoni, P Bazan, R M Tomasino
Vimentin expression, proliferating cell nuclear antigen and flow cytometric factors. Prognostic role in breast cancer
1994 Oct;16(5):365-74
Abstract: A series of 71 patients undergoing surgery for primary breast carcinoma was prospectively studied in order to evaluate the relative weight for four biologic factors (intermediate filament vimentin expression, proliferating cell nuclear antigen [PCNA], flow cytometric DNA ploidy and S-phase fraction) and of several clinicopathologic and biologic features in predicting clinical outcome (disease-free interval). - M Heinrichs, E Striepecke, A Böcking
Quantitative analysis of the neu oncogene in normal and transformed epithelial breast cells by fluorescence in situ hybridization and laser scanning microscopy
1994 Aug;16(4):233-9
Abstract: In this study we evaluated indirect fluorescence in situ hybridization of the neu oncogene in combination with laser scanning microscopy for the detection and quantification of fluorescence signals in single cells. - J Xiao, R Christen, C Minimo, P H Bartels, M Bibbo
An algorithm for automatic tracking of nuclear boundaries
1994 Aug;16(4):240-6
Abstract: An image segmentation algorithm, based on boundary tracking, was introduced to achieve automatic segmentation of nuclei. This will improve reliability and reproducibility for the computer-assisted grading of routinely stained material, especially from biopsies, which often offer only scanty clinical material. Nuclear grading systems using karyometric features were developed earlier. - D Kim, J D Charlton, J M Coggins, J L Mohler
Semiautomated nuclear shape analysis of prostatic carcinoma and benign prostatic hyperplasia
1994 Dec;16(6):400-14
Abstract: Nuclear shape analysis has predicted outcome better than histologic grading in patients with clinically localized prostatic carcinoma. However, the requirement for manual nuclear contour tracing makes the method tedious and slow. - K Sutherland, J W Ironside
Novel application of image analysis to the detection of spongiform change
1994 Dec;16(6):430-4
Abstract: Spongiform change is the characteristic neuropathologic abnormality within the brain in Creutzfeldt-Jakob disease, the most common human spongiform encephalopathy, but traditional methods of evaluating this abnormality are subjective and laborious. - F Gilles, A Gentile, V Le Doussal, E Kahn
Use of texture parameters in the classification of soft tissue tumors
1994 Oct;16(5):315-20
Abstract: Soft tissue tumors are a challenging group of tumors that presents wide morphologic variety. For pathologists, the degree of cellular differentiation, the cellular and nuclear polymorphism and the mitotic rate are major criteria for diagnosis. - D B Holiday, J W McLarty
Identifying disagreement in ordinal ratings within a fixed panel of cytotechnologists
1994 Dec;16(6):381-92
Abstract: In many clinical settings, a fixed number of raters screen specimens with more than two ordinal outcomes (for example, mild, moderate, severe). A design is proposed that facilitates the measurement of both interrater and intrarater agreement and associated trends. - No authors listed
3rd International Conference on the Computerized Cytology and Histology Laboratory. Chicago, Illinois, March 6-9, 1994. Abstracts
1994 Feb;16(1):29-84 - M Aubele, G Auer, U Jütting, U Falkmer, P Gais
Prognostic value of quantitatively measured AgNORs in ductal mammary carcinoma
1994 Jun;16(3):211-8
Abstract: Argyrophilic proteins of nucleolar organizer regions (AgNOR proteins) were stained by a modified silver staining technique. Paraffin sections from 137 cases of invasive ductal carcinoma of the breast were investigated. - M E Boon, H G van der Poel, C J Tan, L P Kok
Effect of embedding methods versus fixative type on karyometric measures
Comparative Study: 1994 Apr;16(2):131-6
Abstract: The influence of fixation and embedding methods in seven urologic tumor samples was studied karyometrically for 12 preparatory techniques. Routine histologic formalin fixation was compared with Carbowax and Kryofix fixatives. - J Billiet, A Criel, J van Vuchelen, M Hidajat, A Van Hoof, A Louwagie, P Michielssen
Relationship between nuclear morphology and DNA content in large cell lymphomas
1994 Oct;16(5):339-44
Abstract: The relationship between DNA content and nuclear morphology in large cell lymphomas (LCLs) was investigated on lymph node imprints. Mean maximum nuclear diameter (mean MND), nuclear shape and chromatin pattern were evaluated microscopically on May-Grünwald-Giemsa-stained slides. - E Lerma, S J Lee, D Tugues, E Oliva, I Gich, J Prat
Ploidy of 36 stromal tumors of the gastrointestinal tract. A comparative study with flow cytometry and image analysis
Comparative Study: 1994 Dec;16(6):435-40
Abstract: DNA ploidy was investigated by flow cytometry (FC) and image analysis (IA) in paraffin-embedded tissue sections from 36 stromal tumors of the gastrointestinal tract. The results of both techniques were correlated with pathologic features of the tumors and survival. - S S Cross, D W Cotton, J C Underwood
Measuring fractal dimensions. Sensitivity to edge-processing functions
1994 Oct;16(5):375-9
Abstract: The fractal dimension is a useful tool in quantitative histology and cytology, and its measurement is easily implemented on computerized image analysis systems. However, the optimal conditions for capture of images and the effect of image-processing functions on the measurement of the fractal dimension have not been reported. - H Kolles, U Hübschen
A dermatologic decision support system that processes lexical knowledge from standard textbooks
1994 Jun;16(3):219-25
Abstract: We present a decision support system that processes lexical fuzzy knowledge from standard pathology textbooks. The user can choose among four methods: - C Olemans, C Piérard-Franchimont, P Delvenne, G E Piérard
Comparative karyometry in Bowen's disease and bowenoid papulosis. Derivation of a nuclear atypia index
1994 Aug;16(4):284-6
Abstract: Bowen's disease and bowenoid papulosis are two diseases that are currently viewed as low grade in situ carcinomas of the epidermis. Their distinction is sometimes disputed. We undertook a morphometric comparison of the size and shape of nuclei in these diseases. - D F Fischler, S Wongbunnate, D A Johnston, R L Katz
DNA content by image analysis. An accurate discriminator of malignancy in pericardial effusions
1994 Jun;16(3):167-73
Abstract: DNA analysis was performed on 20 cytologically equivocal pericardial effusions to determine if it would be a useful ancillary technique in identifying malignancy. Ploidy determination by interactive image analysis showed single diploid DNA peaks, with no cells > 5c, in all eight reactive cases studied. - M Benchaib, C Souchier, M Ffrench, F Remy, P A Bryon
Fluorescence image analysis of plasma cells in monoclonal gammopathies
1994 Apr;16(2):137-44
Abstract: The DNA content of plasma cells was estimated by fluorescence image analysis in monoclonal gammopathies. Image analysis provides the possibility of measuring the bone marrow plasma cell population separately from the other myeloid cells. - F Willemse, M Nap, S C Henzen-Logmans, H F Eggink
Quantification of area percentage of immunohistochemical staining by true color image analysis with application of fixed thresholds
Comparative Study: 1994 Oct;16(5):357-64
Abstract: Most image analysis systems (IAS) use black-and-white cameras. However, true color IASs are considered to be useful for quantification of immunohistologically stained structures. - M J Kube, D A McDonald, J W Quin, M L Greenberg
Use of archival and fresh cytologic material for the polymerase chain reaction. Detection of the bcl-2 oncogene in lymphoid tissue obtained by fine needle biopsy
1994 Jun;16(3):174-82
Abstract: A technique for extracting DNA from archival and fresh tissue from fine needle biopsy (FNB) samples for the polymerase chain reaction (PCR) is described. The method was used to detect the bcl-2 oncogene in various cytologic lymphoid preparations. - J Vila Torres, M Pineda Marfa, M A González Enseñat, J Lloreta Trull
Pathology of the elastic tissue of the skin in Costello syndrome. An image analysis study using mathematical morphology
Case Reports: 1994 Dec;16(6):421-9
Abstract: Only nine cases of Costello syndrome have been described so far. No definitive pathologic abnormalities have been found on skin biopsies. - J L Mohler, J C Metts, X Z Zhang, S J Maygarden
Nuclear morphometry in automatic biopsy and radical prostatectomy specimens of prostatic carcinoma. A comparison
Comparative Study: 1994 Dec;16(6):415-20
Abstract: Nuclear shape analysis performed upon prostatectomy specimens of prostatic carcinoma distinguished individual patients with good and poor prognoses. In order to be useful for prognosis assessment preoperatively, nuclear morphometry must be measured on needle biopsy specimens. - R Bowman, L Hause
Selection of morphometric features that identify responders and nonresponders in stage IV Wilms' tumors
1994 Apr;16(2):90-4
Abstract: Wilms' tumor is the most common renal malignancy of childhood. The use of histologic grade and stage has divided those patients into two main groups, responders and nonresponders, based on the absence or presence of extreme cytologic atypia (anaplasia). - P Sallinen, H Haapasalo, T Kerttula, I Rantala, H Kalimo, Y Collan, J Isola, H Helin
Sources of variation in the assessment of cell proliferation using proliferating cell nuclear antigen immunohistochemistry
Comparative Study: 1994 Aug;16(4):261-8
Abstract: The reproducibility in quantitation of proliferation activity, determined using the monoclonal antibody 19A2 to proliferating cell nuclear antigen (PCNA), was tested in visual and computer-assisted analyses of brain tumor material. - E M Wojcik, R L Katz, T V Fanning, D A Johnston
Morphonuclear comparison of the mantle zone and diffuse subtypes of mantle cell lymphoma
Comparative Study: 1994 Aug;16(4):287-92
Abstract: Mantle cell lymphoma (MCL) is a distinct type of non-Hodgkin's lymphoma, which, based on the architectural pattern, has been divided into mantle zone (MCL-MZ), allegedly associated with longer survival, and diffuse (MCL-D) subtypes. - M Sati, G J Laroye
A simple algorithm for measuring the concavity/convexity ratio and lobe counting of a closed curve. With a review of the literature on form factors measuring concavity
1994 Aug;16(4):269-83
Abstract: The algorithm described here identifies concave and convex segments of a closed contour by vector algebra, thereby defining the shape of a two-dimensional object in multiple ways: (1) as a ratio of total concave vs. - J A Balko, L C Tao
An expert system for teaching cytopathologic diagnosis of lung cancers
1994 Oct;16(5):321-31
Abstract: An expert system for teaching cytopathologic skills is described. It uses Tao's cytologic criteria as graded feature parameters for comparison against a set of expert descriptors of known carcinomas. - J T Donovan, W Shiraki, P S Pekow, C N Otis
Reliability of quantitating proliferative cell nuclear antigen in epithelial ovarian tumors
1994 Apr;16(2):85-9
Abstract: Sources of variability in quantitating proliferative cell nuclear antigen (PCNA) by image analysis were evaluated in paraffin sections of 18 ovarian tumors of low malignant potential (LMP) and grade 1 (G1) carcinomas.