Volume 1 (1985) Issue 1 - Issue 6
- P Kendall Jr
High-resolution automated microscopy
Editorial: 1985 Mar;7(1):4-5 - L G Koss
High resolution automated microscopy
Editorial: 1985 Mar;7(1):2-3 - O E Iversen, O D Laerum
Ploidy disturbances in endometrial and ovarian carcinomas. A review
Review: 1985 Dec;7(4):327-36
Abstract: A survey of DNA and chromosome aberrations in human endometrial and ovarian carcinomas is presented, including data obtained by chromosome analysis, absorption cytophotometry and flow cytometry. Nearly all of the cancers showed some structural or numerical chromosomal abnormalities.
- D Wittekind
Standardization of dyes and stains for automated cell pattern recognition
Review: 1985 Mar;7(1):6-30
Abstract: An extensive review is presented of the various factors involved in the standardization of dyes and stains for cytologic material, which is a crucial point in achieving objective and reproducible measurements of cells for recognition by automated microscopy (high-resolution cell image analysis; automated cell pattern recognition [ACPR]). - M Oberholzer, L Bianchi, P Dalquen, L Landmann, P U Heitz
Stereology in the extraction of information from images
1985 Sep;7(3):197-204
Abstract: Some important aspects for information extraction by stereology from images in surgical and experimental pathology are discussed. The relationship between stereology and morphometry is briefly discussed, with the most important conditions for stereologic analysis in pathology pointed out. - V M Kosma, Y Collan, T Kulju, M L Aalto, E Jantunen, J Karhunen, K Selkäinaho
Reproducibility of morphometric measurements of amyloid after various staining methods
Comparative Study: 1985 Dec;7(4):267-70
Abstract: Four histologic staining methods used for detecting amyloid (Congo red, viewed in both normal and polarized light, Sirius red, Crystal violet and Thioflavine T) were applied to heart muscle autopsy samples from 19 patients who suffered from amyloidosis.
- M Bibbo, P H Bartels, H E Dytch, G L Wied
Ploidy patterns in cervical dysplasia
Comparative Study: 1985 Sep;7(3):213-7
Abstract: The ploidy patterns determined for groups of patients with cervical dysplasia (cervical intraepithelial neoplasia [CIN]) were subjected to statistical analysis. The patterns were based on the measurement of at least 100 Feulgen-stained nuclei from 30 patients with normal cervices, 10 cases of CIN I, 18 cases of CIN II and 33 cases of CIN III. - G F Bahr, U V Mikel
The distribution of nuclear mass, volume and dimensions
1985 Jun;7(2):119-25
Abstract: Our own measurements and a review of measurements presented in the literature showed that the mass, volume and dimensions of cell nuclei are distributed with a skew towards higher values, a distribution that can best be described as lognormal. - O Niklasson, G Skude, R Johansson, N Stormby
Screening for endometrial carcinoma by endouterine aspiration cytology with analysis of tumor markers in the aspirates. Development and application of a new screening technique
Comparative Study: 1985 Mar;7(1):61-8
Abstract: A new endometrial aspiration technique for office screening of endometrial carcinoma without anesthesia was studied. In patients without apparent signs of cervical canal stenosis, all of whom were 50 years of age or older, the aspiration attempt was successful in 96%. The aspirates were used for cytologic study and tumor marker assays. - M Bibbo, P H Bartels, H E Dytch, G L Wied
Ploidy measurements by high-resolution cytometry
1985 Jun;7(2):81-9
Abstract: Technology has made DNA ploidy assessment a clinically and economically feasible procedure, 30 years after its clinical potential was established. This paper reviews the history of ploidy measurements, DNA cytophotometry, DNA interpretation and the prognostic value of nuclear DNA analysis in various anatomic sites. - J E Weber, T Nielsen
Multinomial distributions in ploidy analysis
1985 Jun;7(2):140-52
Abstract: Ploidy patterns can be summarized in the form of a vector of proportions representing the frequency of occurrence of DNA contents in specified intervals. Data represented in this way can be analyzed statistically using the multinomial distribution. Properties of the multinomial distribution and computational difficulties that arise in its application are considered. - P H Bartels, J E Weber, M Bibbo
Ploidy pattern analysis. Statistical considerations
1985 Jun;7(2):126-30
Abstract: Availability of large data sets of ploidy measurements makes it possible to study ploidy patterns for the diagnostic and prognostic clues they can provide. Appropriate statistical analyses can improve the accuracy and precision of these studies.
- Y S Fu, T L Hall
DNA ploidy measurements in tissue sections
1985 Jun;7(2):90-6
Abstract: Nuclear DNA ploidy measurements based on tissue sections, although technically tedious and time-consuming, can provide useful diagnostic and prognostic information. Methods for minimizing distributional errors and optimizing interpretation of DNA histograms are presented, and the diagnostic and prognostic significance of DNA ploidy measurements in gynecologic cancer and its precursors is reviewed. - J Juan, F Sigaux, G Flandrin
Automated classification of lymphoid cells
1985 Mar;7(1):38-46
Abstract: A morphometric study using a commercially manufactured automated analyzer was carried out on 18,742 circulating blood lymphoid cells obtained from patients suffering from a lymphoid blood disorder and from healthy controls. - B Lambert, D Rousseau, Y Lepage, M St-Onge
Colpohistologic correlations. A computerized study
1985 Mar;7(1):47-51
Abstract: Data from 300 colposcopic cases were computerized to study the correlations between the colposcopic observations and the histologic and other data. Colposcopy was shown to be most useful in women under 35 years of age. - K K Kayser, F Modlinger, K Postl
Quantitative low-resolution analysis of colon mucosa
Comparative Study: 1985 Sep;7(3):205-12
Abstract: A general concept is presented for the analysis of histopathologically adenomatous structures at low levels of magnification. The algorithm is based upon structures of glands, not upon structures of single cells. - R L Katz, T S Johnson, K D Williamson
Comparison of cytologic and acridine-orange flow-cytometric detection of malignant cells in human body cavity fluids
Comparative Study: 1985 Sep;7(3):227-35
Abstract: Flow cytometrically (FCM) derived DNA and RNA profiles were studied in acridine orange (AO)-stained body cavity fluid (BCF) specimens obtained from 78 patients with various solid tissue and hematologic malignancies. - M R Fiola
Peripheral nerve morphometry for daily practice
Case Reports: 1985 Dec;7(4):299-304
Abstract: Quantitative methods for the routine diagnostic analysis of sural nerve biopsies were evaluated. Semiautomatic image analysis was used to compare three sampling methods and to test the accuracy and reproducibility of measurements of various elements in biopsy specimens from seven cases of paraproteinemic neuropathy. - T Mattfeldt, W Neurohr, A Müller, K Klinga
Stereologic correlates of steroid receptor concentration in invasive ductal breast cancer
1985 Dec;7(4):310-4
Abstract: The hypothesis was tested that morphometric parameters of tumor cell nuclei correlate with the steroid receptor concentration in mammary carcinoma.
- J E Weber, B A Baldessari, P H Bartels
Test statistics for detecting aneuploidy and hyperdiploidy
1985 Jun;7(2):131-9
Abstract: Possible approaches to the analytical evaluation of ploidy patterns are discussed, and two specific problems are considered: detection of early onset of aneuploidy and detection of moderate hyperdiploidy. - Y Usson, G Drouet d'Aubigny
Multimodal frequency distribution analysis of peripheral nerves
1985 Dec;7(4):294-8
Abstract: In morphometric studies of peripheral nerves, the statistical analysis of such data as axon diameters is complicated by the presence of multimodal distributions. Nerve fiber diameters, for example, cannot be analyzed by classical parametric tests, and such descriptive statistics as mean or variance lose much of their usefulness. - M Devonec, B Fontaniere, N Blanc-Brunat, E Muchada, J M Dubernard
Simultaneous staining of nuclear DNA and blood group cell surface antigens on cells from bladder irrigation fluid
1985 Mar;7(1):69-71
Abstract: Simultaneous staining of nuclear DNA and blood group cell-surface antigens is proposed as a means of studying the prognosis of superficial bladder cancer. The quality of the results obtained on urothelial cells from bladder irrigation fluid, as shown by fluorescence microscopy, suggests that this staining technique may be suitable for flow cytometry. - D R Barres, M A Duhr, Y A Boivin
Discrimination between precancerous and cancerous lesions of the uterine cervix by DNA measurements on tissue sections
1985 Dec;7(4):320-6
Abstract: Morphologically typical uterine cervical biopsies were separated into normal cervices, condylomas, and cervical intraepithelial neoplasias (CIN) grades I, II, and III. At least 100 nuclei per lesion were measured on 4-micron Feulgen-stained sections using a Zeiss microspectrophotometer, with a variant of the plug method used to compute the nuclear DNA content. - U V Mikel, W N Fishbein, G F Bahr
Some practical considerations in quantitative absorbance microspectrophotometry. Preparation techniques in DNA cytophotometry
1985 Jun;7(2):107-18
Abstract: An experimental review of the Feulgen and gallocyanine-chrome-alum stains for quantitative cytophotometry of DNA in tissue sections yielded information on the preparation and staining of tissue for quantitative absorbance microspectrophotometry. - W W Johnston, M J Borowitz, G M Stuhlmiller, H F Seigler
Expression of a melanoma-tumor-associated antigen as demonstrated by a monoclonal antibody (D6.1) in cytopathologic preparations of human tumor cells
Comparative Study: 1985 Mar;7(1):72-80
Abstract: Immunocytopathologic studies were performed on 79 fine needle aspiration biopsies (FNABs) and effusions from 13 melanomas and 57 other human neoplasms with the monoclonal antibody (MAb) D6.1 raised against a partially purified melanoma-tumor-associated antigen (MTAA). - K Nishimiya, G U Auer, K Erhardt, L G Wiman, H Kato, Y Hayata
Nuclear DNA in histologic sections from squamous-cell carcinoma and adenocarcinoma of the lung
1985 Jun;7(2):153-8
Abstract: The nuclear DNA content in morphologically identified tumor cells was analyzed in 4-micron histologic sections from 58 patients with lung carcinoma who survived for at least five years. - V M Kosma, Y Collan, T Kulju, M L Aalto, E Jantunen, J Karhunen, K Selkäinaho
Reproducibility and variation of morphometric analysis of carcinoembryonic antigen staining in histopathology
1985 Dec;7(4):271-4
Abstract: Using morphometric methods, five pathologists analyzed the positive staining for carcinoembryonic antigen (CEA) in sections from 17 ovarian tumors, with the intraclass correlation coefficient (ICC) and the mean values of the coefficients of variation (CV) used to assess reproducibility and variation. - J W Gamel, J Gleason, H Williams, R Greenberg
Reproducibility of nucleolar measurements in human intraocular melanoma cells on standard histologic microslides
Comparative Study: 1985 Sep;7(3):174-7
Abstract: The standard deviation of nucleolar area (SDNA), as determined by semiautomated measurement of routinely prepared histologic sections, has been shown to be an effective predictor of mortality for human intraocular melanoma. - G L Wied, M Bibbo, H E Dytch, P H Bartels
Computer grading of cervical intraepithelial neoplastic lesions. I. Cytologic indices
1985 Mar;7(1):52-60
Abstract: In an attempt to derive a computer-assisted grading for cervical intraepithelial neoplastic (CIN) lesions, apparent discrepancies with the visual diagnostic evaluation could be explained from the data structure of the objective assessment.
- Y Collan
Stereology and morphometry in histopathology. Principles of application
1985 Dec;7(4):237-41
Abstract: The role of stereology and morphometry (including image analysis) in histopathology is considered. Diagnostic histopathology has its own character, which should be considered when quantitative methods are applied. - M S Dunnill
Some statistical aspects of sampling in morphometry
1985 Dec;7(4):250-5
Abstract: Morphometry requires, in addition to the accurate counting of points, intersections and transections, meticulous attention to sampling. Methods are described and discussed for ensuring that adequate samples are obtained for a given standard error. - M Raphael, C Lesty, L Nonnenmacher, A Delcourt, V Missenard-Leblond, J L Binet
Morphometric characterization of nuclei in non-Hodgkin's malignant lymphoma
1985 Dec;7(4):283-7
Abstract: In addition to the nuclear area and a form factor, four morphometric parameters of nuclear shape (ID, R1, R2, and ND), obtained by the application of the principles of mathematical morphology, were used to characterize the nuclear contours in non-Hodgkin's malignant lymphomas. - G U Auer, M Bäckdahl, G M Forsslund, U G Askensten
Ploidy levels in nonneoplastic and neoplastic thyroid cells
1985 Jun;7(2):97-106
Abstract: DNA levels in nonneoplastic and neoplastic human thyroid cells were studied by slide-cytophotometric and flow-cytophotometric techniques. - G Mikuz, F Hofstaedter, R Delgado
Extraction of cells from paraffin-embedded tissue sections for single-cell DNA cytophotometry
Comparative Study: 1985 Dec;7(4):343-6
Abstract: A simple technique is presented for the isolation of cells from paraffin-embedded tissues for Feulgen DNA cytophotometric investigations. - S Rosen, W E Mercer
Cytophotometry of breast carcinoma. Acridine-orange DNA microfluorimetry with Giemsa counterstain
1985 Jun;7(2):159-62
Abstract: Investigations have suggested that a correlation exists between DNA ploidy levels and prognosis in human breast carcinoma. Nuclear DNA content can be studied by flow cytometry or cytophotometric analysis. - No authors listed
Proceedings of the Third International Symposium on Morphometry in Morphological Diagnosis. Delft, The Netherlands, September 13-15, 1984
1985 Dec;7(4):237-352 - W F Whimster
Diagnostic morphometry in emphysema and chronic bronchitis
1985 Sep;7(3):183-6
Abstract: A morphometric technique of point counting was developed for macroscopic use in emphysematous lungs and microscopic use in bronchi to obtain actual areas and volumes, as opposed to ratios or percentages, of emphysema and submucosal glands. - M Bins, S Poppema
DNA measurements in thin sections of lymphomas
1985 Dec;7(4):315-9
Abstract: The DNA content of the nuclei of lymphomas and a reactive lymph node was studied by light absorption measurements in Feulgen-stained thin (2 microns) sections of lymph node biopsies, using the TAS image analysis system. - D Komitowski, G Zinser
Quantitative description of chromatin structure during neoplasia by the method of image processing
Comparative Study: 1985 Sep;7(3):178-82
Abstract: Nuclear chromatin is visualized by light microscopy as a mosaic of interchanging regions of low and high optical density (O.D.). The regions of high O.D. are well-defined as chromatin particles; features characterizing these particles enable the description of chromatin structure and the recognition of its changes during neoplasia.
- S H Paplanus, A R Graham, J M Layton, P H Bartels
Statistical histometry in the diagnostic assessment of tissue sections
1985 Mar;7(1):32-7
Abstract: A statistical histometric model to distinguish adenoma of the colon from normal colon is described. The model, based on the number and location of glandular nuclei, with a dependency scheme based upon displacement, was tested and shown to be significant and capable of simulating the architecture of an adenoma. - P Felman, P A Bryon, O Gentilhomme, J P Magaud, A M Manel, B Coiffier, G Lenoir
Burkitt's lymphoma. Distinction of subgroups by morphometric analysis of the characteristics of 55 cell lines
1985 Dec;7(4):275-82
Abstract: In an attempt to clarify the controversy about the distinction between Burkitt's and non-Burkitt's small noncleaved lymphomas, 55 cell lines derived from 48 Burkitt's lymphoma patients were characterized by morphometry on plastic-embedded sections. - W Auffermann, A Böcking
Early detection of precancerous lesions in dysplasias of the lung by rapid DNA image cytometry
1985 Sep;7(3):218-26
Abstract: Hematoxylin-and-eosin-stained cytologic smears of sputum from 28 patients with dysplastic and suspicious cell findings were subjected to DNA image cytometry after Feulgen restaining. - R Ricco, G De Benedictis, C Giardina, P Bufo, L Resta, V Pesce Delfino
Morphometric analytical evaluators of lymphoid populations in nonneoplastic lymph nodes
1985 Dec;7(4):288-93
Abstract: A morphometric analysis using an Apple II microcomputer and shape analytical morphometry (SAM) software was performed of the nuclear shape in the lymphoid population of normal (nonneoplastic) lymph nodes to provide baseline parameters useful for distinguishing between different subtypes of lymphomas. - R Montironi, M Scarpelli, E Pisani, G Ansuini, F Marinelli, G Mariuzzi
Noninvasive papillary transitional-cell tumors. Karyometric and DNA-content analysis
Comparative Study: 1985 Dec;7(4):337-42
Abstract: An investigation was undertaken to quantify and correlate the nuclear and DNA-content modifications in the different grades of noninvasive urothelial papillary carcinoma. - S B Leif, R C Leif, R Auer
The EPICS C analyzer. An ergometrically designed flow cytometer computer system
1985 Sep;7(3):187-91
Abstract: A description is presented of a new flow-cytometer (FCM) computer system, the EPICS C analyzer, which was developed to be used by a moderately trained technician as opposed to an FCM expert. - J Oort
Theoretical aspects of diagnostic histopathology and their relation to morphometry
1985 Sep;7(3):164-6
Abstract: The theoretical aspects of diagnostic histopathology are discussed and their relationship to morphometry is examined. In diagnostic pathology, one tends to look at certain structural features, overlooking other details regarded as unimportant. - A Cornelis, D Van Meerbeek, M Nyssen, F Roels
Specific cytochemical stains in the image analysis of subcellular organelles
1985 Dec;7(4):256-66
Abstract: This paper describes our work concerning densitometry and morphometry of subcellular structures in thin sections. The techniques of automatic image analysis were applied to light and electron microscopic observations of enzymatically stained lysosomes, renal brush borders and mitochondria (in human and rat kidney) and peroxisomes (in human liver). - J Foster
Medical applications of image analysis with the Magiscan 2
1985 Sep;7(3):192-6
Abstract: The previous generation of image analysis machines were capable of processing and analyzing binary, i.e., black and white images, and making measurements and decisions thereon. - F A Langley, C H Buckley, M Tasker
The use of ROC curves in histopathologic decision making
1985 Sep;7(3):167-73
Abstract: The applicability of receiver operator characteristic (ROC) curves to histopathology is presented in three examples, along with a basic discussion of some properties of ROC curves. - V Cavallari, A Maiorana, G Basile, R A Fano
Sympathetic ganglia in diabetes. An ultrastructural and stereologic study
Comparative Study: 1985 Dec;7(4):305-9
Abstract: An ultrastructural and stereologic study was performed on sympathetic ganglia collected at surgery from eight diabetic patients, seven age-matched non-diabetics and six subjects with glucose intolerance.