Volume 18 (2002) Issue 1 - Issue 6
- Leendert H J Looijenga, J Wolter Oosterhuis
Pathobiology of testicular germ cell tumors: views and news
Review: 2002 Oct;24(5):263-79
Abstract: Human germ cell tumors (GCTs) are a heterogeneous group of neoplasms. They can occur in different anatomic locations, predominantly in the gonads (both ovary and testis) and in the midline of the body, including the retroperitoneal, mediastinal and hypothalamus/pineal gland regions. - Esperanza Rosalba Rodríguez-Reyes, Ricardo M Cerda-Flores, Juan Manuel Quiñónez-Pérez, Víctor Velásco-Rodríguez, Elva I Cortés-Gutiérrez
Acetic acid test: a promising screening test for early detection of cervical cancer
Clinical Trial: 2002 Jun;24(3):134-6
Abstract: To determine whether the acetic acid test (AAT) could be used as a screening testfor early detection of cervical cancer. - Aldo V Bono, Maurizio Salvadore, Nicola Celato
Gonadotropin-releasing hormone receptors in prostate tissue
2002 Aug;24(4):221-7
Abstract: To perform an immunohistochemical analysis of gonadotropin-releasing hormone receptors (GnRH-Rs) in archival prostate tissue. - T Paul Hutchinson, Sigurbjorg Gudlaugsdottir
Modelling errors in scoring the darkness of staining
Review: 2002 Apr;24(2):121-4
Abstract: To construct a statistical model for the agreements and disagreements between two observers on darkness of staining. - Ståle Nordgård, Anna Bofin, Bjørn Hagmar
Ki-67 antigen detection in unstained and destained cytologic samples
2002 Aug;24(4):198-204
Abstract: To evaluate the effect of different tissue preservation, fixation and staining procedures on the expression of proliferation-associated Ki-67 antigen in cytologic samples to establish an easy and uniform way to handle cytologic specimens with an automated staining technique. - Michael Samoszuk, Leonard Leonor, Froilan Espinoza, Philip M Carpenter, Orhan Nalcioglu, Min-Ying Su
Measuring microvascular density in tumors by digital dissection
2002 Feb;24(1):15-22
Abstract: To develop and validate a digital dissection techniquefor measuring the cross-sectional area of blood vessels in histologic sections of tumors routinely stained with hematoxylin and eosin. - Petros Karakitsos, Aspassia Kyroudes, Abraham Pouliakis, Efi Botsoli Stergiou, Zannis Voulgaris, Christos Kittas
Potential of the learning vector quantizer in the cell classification of endometrial lesions in postmenopausal women
2002 Feb;24(1):30-8
Abstract: To investigate the potential of artificial neural networks for cell identification in endometrial lesions from postmenopausal women. - Jeanne Adiwinata Pawitan, Retno Wilujeng Susilowati
Normal value for the direct micronucleus test on peripheral blood mononuclear cells from healthy donors
2002 Dec;24(6):342-4
Abstract: To obtain the normal value of micronuclei in peripheral blood mononuclear cells. - Timothy W King, Eric M Brey, Adel A Youssef, Carol Johnston, Charles W Patrick Jr
Quantification of vascular density using a semiautomated technique for immunostained specimens
2002 Feb;24(1):39-48
Abstract: To develop a semiautomated, quantitative techniquefor the assessment of vascular density in immunohistochemically stained tissue sections using diaminobenzidine tetrahydrochloride (DAB) and hematoxylin as chromagens. - Edmond Kahn, Christine Tessier, Gérard Lizard, Anne Petiet, Frédéric Brau, Olivier Clément, Frédérique Frouin, Jean-René Jourdain, Françoise Guiraud-Vitaux, Nicole Colas-Linhart, Nathalie Siauve, Charles-André Cuenod, Guy Frija, Andrew Todd-Pokropek
Distribution of injected MRI contrast agents in mouse livers studied by confocal and SIMS microscopy
2002 Oct;24(5):295-302
Abstract: To localize magnetic resonance imaging (MRI) contrast agents injected intravenously into mouse livers. - Anju Mehrotra, Madhu Mati Goel, Kamlesh Singh
Ki-67 and AgNOR proliferative markers as diagnostic adjuncts to fine needle aspiration cytology of thyroid follicular lesions
2002 Aug;24(4):205-11
Abstract: To differentiate hyperplastic nodules (HPN), follicular adenoma (FA) and follicular carcinoma (FCA) of the thyroid by cytomorphologic features combined with argyrophilic nucleolar organizer regions (AgNORs) and Ki-67 proliferative markers on fine needle aspiration cytology. - Mükerrem Safali, Bülent Celasun, Omer Günhan
DNA cytometry in pleomorphic adenomas with cytologic atypia
2002 Dec;24(6):325-30
Abstract: To investigate the nuclear DNA content of pleomorphic adenomas with cytologic atypia. - John W Bishop, Raphael Marcelpoil, Joachim Schmid
Machine scoring of Her2/neu immunohistochemical stains
2002 Oct;24(5):257-62
Abstract: To establish the feasibility of a machine scoring method for her2/neu immunohistochemistry in samples of breast carcinoma. - Björn Stenkvist
A possible new principle for adjuvant treatment of cancer in humans revealed by image analysis cytology
Comment: 2002 Oct;24(5):303-4 - Geula Klorin, Ruth Keren
Prognostic evaluation of breast cancer in cytologic specimens
2002 Feb;24(1):49-53
Abstract: To evaluate prognostic factors in breast cancer using cytologic samples and to determine the correlation between those factors and ploidy. - Fabio Grizzi, Barbara Franceschini, Barbara Barbieri, Nicoletta Gagliano, Beatrice Arosio, Maurizio Chiriva-Internati, Giorgio Annoni, Nicola Dioguardi
Mast cell density: a quantitative index of acute liver inflammation
2002 Apr;24(2):63-9
Abstract: To evaluate mast cell (MC) density, in liver tissues taken from young and aging rats treated with carbon tetrachloride (CCl4) or untreated, as a quantitative marker of acute liver inflammation and to investigate whether the density of MCs varied with the rats' age. - Manish Powari, Neelam Varma, Subhash Varma, Ram Kumar Marwaha, Harpreet Sandhu, Nirmal Kumar Ganguly
Flow cytometric characterization of phenotype, DNA indices and p53 gene expression in 55 cases of acute leukemia
Clinical Trial: 2002 Jun;24(3):159-65
Abstract: To characterize the phenotype of acute leukemia cases using flow cytometry, to detect mixed lineage cases and to use DNA index determination, including S-phase fraction (SPF) and p53 detection, to find if there was any correlation of SPF and p53 expression with outcome. - Ling-Xi L Li, Kerry A Crotty, Allan A Palmer, Jillian J Kril, Roger Stankovic, Richard A Scolyer, Stanley W McCarthy
Differentiating benign nevi from malignant melanoma using DNA microdensitometry and karyometry and maturation: a zonal comparison, correlation and multivariate analysis
2002 Aug;24(4):234-43
Abstract: To evaluate the diagnostic effectiveness of cytometric features of DNA microdensitometry, karyometry (nuclear morphometry) and maturation and their combinations in separating benign nevi from malignant melanomas. - Francisco A R Garcia, John R Davis, Kenneth Hatch, David S Alberts, Deborah Thompson, Peter H Bartels
Karyometry in endometrial adenocarcinoma of different grades
2002 Apr;24(2):93-102
Abstract: To characterize nuclei from adenocarcinoma of the endometrium of different grades in order to establish whether significantly different nuclear subpopulations exist. - António E Pinto, Evelina Mendonça, Saudade André, Sónia D Nóbrega, Jorge Soares
Independent prognostic value of hormone receptor expression and S-phase fraction in advanced breast cancer
2002 Dec;24(6):345-54
Abstract: TO assess the prognostic value of immunocytochemically assessed hormone receptor expression and DNA flow cytometry data in advanced breast cancer. - Maohuai Chen, Joseph C K Lee, Xianying Wu, Shikyuen Lo
Morphometric analysis of AgNORs in nonkeratinizing carcinoma and adjacent normal epithelia in the nasopharynx
2002 Jun;24(3):173-7
Abstract: To evaluate the proliferative activity of different types of nonkeratinizing carcinoma and adjacent normal epithelia in the nasopharynx by the quantitative assessment of argyrophilic nucleolar organizer region (AgNOR) proteins. - Michael H Weinstein
An objective measure of architectural complexity and proliferative index in Gleason pattern 3 prostate cancer
2002 Oct;24(5):280-8
Abstract: To objectively characterize the architectural spectrum of Gleason pattern 3 prostate cancer (PCA) in a biologically meaningful manner. - Ling-Xi L Li, Kerry A Crotty, Allan A Palmer, Jillian J Kril, Richard A Scolyer, Stanley W McCarthy
Melanin bleaching in argyrophilic staining of AgNORs in pigmented lesions: a morphometric evaluation
2002 Jun;24(3):166-72
Abstract: To establish a procedure that can effectively bleach melanin from pigmented lesions without affecting quantification of argyrophilic staining of nucleolar organizer regions (AgNORs). - Marco G W Bol, Jan P A Baak, Emiel A M Janssen, Nelly G Breeuwsma, Peter C de Bruin
Further testing of morphometric grading in T(A,1) urothelial carcinomas of the urinary bladder and the additional value of p53 immunoquantitation
2002 Apr;24(2):114-20
Abstract: To analyze the value for grading of a previously developed quantitative morphometric/cytometric multivariate grading model (consisting of the mean nuclear area of the 10 largest nuclei (MNA-10, mitotic activity index = MAI and Ki-67 area% = Ki-67) in two new independent test sets of urothelial carcinomas (UCs) of the urinary bladder and to evaluate the additional value of p53 area% (p53) in this model. - Ji Shin Lee, Hyung Seok Kim, Jong Jae Jung, Min Cheol Lee, Chang Soo Park
Angiogenesis, cell proliferation and apoptosis in progression of cervical neoplasia
2002 Apr;24(2):81-4
Abstract: To investigate changes in angiogenesis, cell proliferation and apoptosis in the successive steps of cervical neoplasia and to analyze their interrelationship. - Logasundaram Rajesh, Pranab Dey, Kusum Joshi
Automated image morphometry of lobular breast carcinoma
2002 Apr;24(2):85-92
Abstract: To analyze the role of automated image morphometry (AIM) in distinguishing infiltrating lobular carcinoma (ILC) of the breast from benign, borderline and infiltrating ductal carcinoma (IDC). - Susan K Keesee, Richard Domanik, Bruce Patterson
Fully automated proteomic detection of cervical dysplasia
2002 Jun;24(3):137-46
Abstract: To evaluate the efficacy of two biomarkers, transferrin receptor (TfR) and epidermal growth factor receptor (EGFR), for the early detection of cervical dysplasia and to explore the relationship of E5, one of three viral oncogenes expressed by the human papillomavirus (HPV), to TfR and EGFR. - Ragip Ortaç, Safiye Aktaş, Gülden Diniz, Ayşe Erbay, Canan Vergin
Prognostic role of natural killer cells in pediatric mixed cellularity and nodular sclerosing Hodgkin's disease
2002 Oct;24(5):249-53
Abstract: To study natural killer cells' spontaneous cytotoxic capacity against tumor cells and their prognostic significance in classical Hodgkin's disease. - Lars-Christian Horn, Georgios Raptis, Hans Nenning
DNA cytometric analysis of surgically treated squamous cell cancer of the uterine cervix, stage pT1b1-pT2b
2002 Feb;24(1):23-9
Abstract: To determine the utility of DNA content and DNA-related variables of proliferative activity regarding prognosis in cervical cancer. - Zaklina Mijovic, Dragan Mihailovic
Discriminant analysis of image karyometric variables in benign and malignant melanocytic skin lesions
2002 Dec;24(6):314-6
Abstract: To perform a quantitative analysis to identify which of 7 nuclear morphometry-related variables are of diagnostic value in distinguishing benign from malignant melanocytic skin lesions. - Sushmita Ghoshal, Pranab Dey, Uma Nahar Saikia, Suresh Chandra Sharma
Prognostic value of p53 overexpression in head and neck carcinomas: a pilot study
2002 Oct;24(5):254-6
Abstract: To correlate p53 overexpression in squamous cell carcinoma of the head and neck region with the outcome of treatment. - Paul A Kay, Darren L Riehle, John C Cheville, Christine M Lohse, V Shane Pankratz, Eunice M Hill, Thomas J Sebo
Comparison of quantitative histomorphometry and DNA ploidy in tissue sections of prostate carcinoma
Comparative Study: 2002 Feb;24(1):7-14
Abstract: To examine the ability of quantitative histomorphometry to predict DNA ploidy of prostate carcinoma in biopsy tissue sections assigned after quantitation by nuclear digital image analysis. - Maria C deGuzman, Nagabhushana Prabhu, Harvey Cramer
Automated breast cancer diagnosis based on fine needle aspiration
2002 Dec;24(6):305-13
Abstract: To design and analyze an automated diagnostic system for breast carcinoma based on fine needle aspiration (FNA). - Harry Harms, Manfred Hofmann, Ilka Ruschenburg
Fine needle aspiration of the thyroid: can an image processing system improve differentiation?
2002 Jun;24(3):147-53
Abstract: To differentiate thyroid nodules by means of fine needle aspiration with subsequent computer-aided diagnosis. - Francis Ikpatt, Teijo Kuopio, Aras Erekul, Yrjö Collan
Apoptosis in breast cancer: Nigerian vs. Finnish material
2002 Jun;24(3):154-8
Abstract: To evaluate apoptotic activity in breast cancer from Nigerian (n = 300) and Finnish (n=285) women. - Peng-Hui Wang, Wen-Yuann Shyong, Chi-Hung Lin, Yann-Jang Chen, Ywan Feng Li, Hsiang-Tai Chao, Chiou-Chung Yuan
Analysis of genetic aberrations in uterine adenomyosis using comparative genomic hybridization
2002 Feb;24(1):1-6
Abstract: To use comparative genomic hybridization (CGH) to analyzefrozen tissuesfrom adenomyosis cases to discover and map genomic regions for chromosomal gains and losses. - Maohuai Chen, Joseph C K Lee, Shikyuen Lo
Quantitative AgNOR assessment of cancer cell populations in undifferentiated nasopharyngeal carcinoma
2002 Apr;24(2):89-92
Abstract: To evaluate the proliferative activity of cancer cell populations in undifferentiated nasopharyngeal carcinoma (NPC) by quantitative assessment of argyrophilic nucleolar organizer regions (AgNOR) proteins. - Manabu Hattori, Eiji Ohno, Hirokuni Kakinuma, Toyoaki Uchida, Hiroyuki Kuramoto
Comparative image analysis of cells in voided and catheterized urine
Clinical Trial: 2002 Jun;24(3):125-33
Abstract: To objectively evaluate the difference in cytologic findings between specimens of voided and catheterized urine by using a comparative image analysis device, CAS200. - Aleksandra Mojsilovic, Bernice Rogowitz, Jose Gomes, Thomas S Deisboeck
Analysis and 3D reconstruction of heterogeneity in malignant brain tumors: an interdisciplinary case study using a novel computational visualization approach
Case Reports: 2002 Jun;24(3):178-84
Abstract: To explore how a multidisciplinary approach, combining modern visualization and image processing techniques with innovative experimental studies, can augment the understanding of tumor development. - Carlos Alberto Mandarim-de-Lacerda, Agata Cristie Mello Madeira, Leila Maria Meirelles Pereira
Cardiomyocyte volume-weighted nuclear volume and spironolactone therapy in spontaneously hypertensive rats
2002 Dec;24(6):331-6
Abstract: To evaluate the blood pressure (BP) and cardiomyocyte nuclei hypertrophy of spontaneously hypertensive rats (SHR) treated with spironolactone and with spironolactone and an angiotensin-converting enzyme inhibitor and calcium channel blocker. - Sudip K Sarker
Mean nuclear area of fine needle aspirates of primary preoperative palpable breast carcinoma using image cytometry
2002 Apr;24(2):85-8
Abstract: Early, operable breast cancers in appropriate patients are increasingly being treated preoperatively using neoadjuvant chemotherapy. A good response rate is seen with high grade tumors. Nuclear size, which may reflect the grade of the tumor, is also of possible prognostic value in breast cancer. - Peter H Bartels, James Ranger-Moore, M Suzanne Stratton, Paul Bozzo, Janine Einspahr, Yun Liu, Deborah Thompson, David S Alberts
Statistical analysis of chemopreventive efficacy of vitamin A in sun-exposed, normal skin
Clinical Trial: 2002 Aug;24(4):185-97
Abstract: To develop numeric, statistically secured measures of chemopreventive efficacy and to derive procedures with high sensitivity of detection. - Els I G B De Brauwer, Jan A Jacobs, Fred Nieman, Cathrien A Bruggeman, Marjolein Drent
Bronchoalveolar lavage fluid differential cell count. How many cells should be counted?
2002 Dec;24(6):337-41
Abstract: To investigate the number of cells to be counted in cytocentrifuged bronchoalveolar lavage (BAL) fluid preparations in order to reach a reliable enumeration of each cell type. - Panagiota Spyridonos, Dionisios Cavouras, Panagiota Ravazoula, George Nikiforidis
Neural network-based segmentation and classification system for automated grading of histologic sections of bladder carcinoma
2002 Dec;24(6):317-24
Abstract: To develop an image analysis system for automated nuclear segmentation and classification of histologic bladder sections employing quantitative nuclear features. - Alfredo Santinelli, Mariagrazia Baccarini, Paola Colanzi, Daniela Stramazzotti, Guidalberto Fabris
Immunohistochemical evaluation of HER-2/neu expression in infiltrating breast carcinoma: a study of reproducibility
2002 Feb;24(1):54-62
Abstract: To determine interobserver and intraobserver reproducibility in the assessment of the HercepTest- and TAB250-immunostained slides. - Edmond Kahn, Gérard Lizard, Serge Monier, Ginette Bessède, Frédérique Frouin, Philippe Gambert, Andrew Todd-Pokropek
Flow cytometry and factor analysis evaluation of confocal image sequences of morphologic and functional changes occurring at the mitochondrial level during 7-ketocholesterol-induced cell death
2002 Dec;24(6):355-62
Abstract: To analyze functional and morphologic alterations that occur at the mitochondrial level by flow cytometry and laser scanning confocal microscopy (CLSM) combined with factor analysis of biomedical image sequences (FAMIS). - Osamu Nunobiki, Misako Sato, Emiko Taniguchi, Weihua Tang, Misa Nakamura, Hitoshi Utsunomiya, Yasushi Nakamura, Ichiro Mori, Kennichi Kakudo
Color image analysis of cervical neoplasia using RGB computer color specification
2002 Oct;24(5):289-94
Abstract: To establish quantitative color image analysis for cytology, red, green and blue (RGB) color specification was applied to Papanicolaou-stained cervical smears. - Edmond Kahn, Philippe Coullin, Frédérique Frouin, Andrew Todd-Pokropek, Alain Bernheim
Improved confocal characterization of targets in nuclei of cytogenetic preparations
Comparative Study: 2002 Jun;24(3):178-84
Abstract: To show that cellular preparations requiring depth analysis of different domains stained by molecular cytogenetic methods (fluorescence in situ hybridization and primed in situ) can be improved by regularized factor analysis of medical image sequences (FAMIS) to isolate fluorescent probes by means of intensity depth profiles of fluorochromes, to track relevant DNA sequences (cosmids and centromeres) in cell nuclei during interphase and to improve the use of cytogenetic techniques resulting in flat preparations of whole cells that are assumed to preserve probe access to their targets. - Rajan Arora, Kusum Joshi, Raje Nijhawan, Bishan D Radotra, Suresh C Sharma
Angiogenesis as an independent prognostic indicator in node-negative breast cancer
2002 Aug;24(4):228-33
Abstract: To quantitate tumor angiogenesis in carcinoma of the breast (stage T2N0M0) by computerized image analysis of CD-31-stained histologic sections and, keeping in view the heterogeneity of tumors, to assess which areas of neovascularization provide the best prognostic indicator. - Susan S Williams, Robert L Zimmerman, Marluce Bibbo
BCA-225 immunostain: is it of value in liver fine needle aspiration?
2002 Oct;24(5):245-8
Abstract: To determine the utility of BCA-225 immunostain in differentiating hepatocellular carcinoma (HCC) from other malignant tumors in liverfine needle aspiration specimens. - Ziva Pohar-Marinsek, Marija Us-Krasovec, Rastko Golouh, Mario Zganec
Value of image cytometry in the subclassification of rhabdomyosarcoma
2002 Aug;24(4):212-20
Abstract: To test the discriminatory capability of nuclear features in the subclassification of rhabdomyosarcoma (RMS) and especially to differentiate embryonal from alveolar RMS. - Pranab Dey, Manish Powari
Mean nuclear volume in cervical intraepithelial neoplasia and carcinoma
2002 Apr;24(2):70-2
Abstract: To correlate three-dimensional nuclear size (mean nuclear volume) estimated by the stereologic intercept methodfor objective classification of cervical intraepithelial neoplasia (CIN) and carcinoma.